BAG2 antibody

Principal name

BAG2 antibody

Alternative names for BAG2 antibody

BAG-2, BAG family molecular chaperone regulator 2, Bcl-2-associated athanogene 2

SwissProt ID

O95816 (Human), Q3ZBG5 (Bovin), Q91YN9 (Mouse)

Gene ID

9532 (BAG2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of BAG2 antibody

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

General readings

Dai Q, Qian SB, Li HH, McDonough H, Borchers C, Huang D, Takayama S, Younger JM, Ren HY, Cyr DM, Patterson C.
Regulation of the cytoplasmic quality control protein degradation pathway by BAG2.
J Biol Chem. 2005 Nov 18;280(46):38673-81. Epub 2005 Sep 16.
PMID: 16169850

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG2 (C-term) antibody

TA302728 (0.3µg/ml) staining of Hela lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG2 antibody

BAG2 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

BAG2 antibody

Western Blot analysis of BAG2 expression in transfected 293T cell line (H00009532-T01) by BAG2 MaxPab polyclonal antibody.

Lane 1: BAG2 transfected lysate(23.21 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

BAG2 antibody

Immunoprecipitation of BAG2 transfected lysate using anti-BAG2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BAG2 MaxPab mouse polyclonal antibody (B01) (H00009532-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

BAG2 antibody

BAG2 monoclonal antibody (M01), clone 6E12 Western Blot analysis of BAG2 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 6E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BAG2 antibody

Immunofluorescence confocal microscopy of BAG2. Using BAG2 polyclonal antibody ( Cat # PAB0329 ) at 1 : 2000. HeLa cells stably transfected with Myc-CHIP ( carboxyl terminus of Hsp70-interacting protein ) and growing on coverslips were left untreated ( A ) or heat shocked at 42 degrees for 30 min ( B ) . Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al ( 2005 ) for additional information on HeLa cells stably transfected with Myc-CHIP. Rabbit Hu C, ICC/IF, IP, P, WB
50 µl / €400.00
  Abnova Taiwan Corp.

BAG2 antibody

BAG2 polyclonal antibody ( Cat # PAB6054 ) staining ( 0.3 µg/ml ) of HeLa lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

BAG2 antibody

Human Kidney; Rabbit Anti-BAG2 Antibody. Catalog Number: ARP42177. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; BAG2 antibody - C-terminal region (ARP42177_T100) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

BAG2 antibody

BAG2 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

BAG2 antibody

Western Blot: BAG2 Antibody [NB100-1776] - NB100-1776 (0.3ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

BAG2 antibody

Western Blot: BAG2 Antibody [NBP1-59087] - This Anti-BAG2 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1.25ug/ml. Rabbit Purified Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BAG2 (1-211, His-tag)

BAG2 Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

BAG2 (1-211, His-tag)

BAG2 Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

BAG2

BAG2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

BAG2

BAG2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

BAG2

BAG2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

BAG2

BAG2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

BAG2

BAG2 Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

BAG2

BAG2 Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

Antibody Pairs

Catalog No.    

BAG2 (Human) IP-WB Antibody Pair

BAG2 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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