BAG3 antibody

Principal name

BAG3 antibody

Alternative names for BAG3 antibody

BAG-3, BIS, Docking protein CAIR-1, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BAG family molecular chaperone regulator 3

SwissProt ID

O95817 (Human), Q9JLV1 (Mouse)

Gene ID

9531 (BAG3)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C)

Background of BAG3 antibody

The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neurol differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhani et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additiol domains which eble them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.

General readings

Kyratsous CA., et al. (2008) Proc Natl Acad Sci U S A. 105(52):20912-7.
Virador VM., et al. (2009) PLos One. 4(4):e5136.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG3 antibody

(0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG3 antibody

Western blot analysis of extracts from 293 cells, using BAG3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG3 antibody

Predicted band size: 62kDa. Positive control: Mouse muscle tissue and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Mouse muscle tissue lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG3 antibody

Predicted band size: 62kDa. Positive control: Mouse muscle tissue, K562 cell and mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Mouse muscle tissue lysate Lane2: K562 cell lysate Lane3: Mouse heart tissue lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG3 antibody

TA326328 (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG3 antibody

Western Blot: BAG3 Antibody [TA337170] - Analysis of BAG3 using TA337170 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and d Rabbit Purified Hu, Ms C, IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

BAG3 (C-term) antibody

Immunohistochemistry Image: Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BAG3 antibody

BAG3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

BAG3 antibody

Detection limit for recombinant GST tagged BAG3 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2b 5A8 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

BAG3 antibody

BAG3 Mouse IgG2b 5A8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

BAG3 antibody

Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections. Using BAG3 polyclonal antibody ( Cat # PAB0330 ) at 1 : 2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain. Rabbit Hu, Ms C, IP, P, WB
50 µl / €470.00
  Abnova Taiwan Corp.

BAG3 antibody

Human HT1080; WB Suggested Anti-BAG3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate. BAG3 is supported by BioGPS gene expression data to be expressed in HT1080.; BAG3 antibody - middle region (ARP48251_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

BAG3 antibody

BAG3 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

BAG3 antibody

Western blot analysis of BAG3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BAG3 antibody

Western blot analysis of BAG3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BAG3 antibody

Western blot analysis of BAG3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BAG3 antibody

Western Blot: BAG3 Antibody [NBP1-70418] - This Anti-BAG3 antibody was used in Western Blot of HT1080     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

BAG3 antibody

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Immunohistochemical staining of human prostate shows distinct positivity in basal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

BAG3 antibody

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Immunohistochemical staining of human oral mucosa shows strong positivity in squamous epithelial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BAG3

BAG3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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