BAG4 antibody

Principal name

BAG4 antibody

Alternative names for BAG4 antibody

BAG-4, SODD, BAG family molecular chaperone regulator 4, Bcl-2-associated athanogene 4, Silencer of death domains

SwissProt ID

O95429 (Human), Q8CI61 (Mouse)

Gene ID

9530 (BAG4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of BAG4 antibody

Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands. Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation. SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.
SODD (Silencer of Death Domain) is an important regulator of TNF induced apoptotic pathway. It associates with TNF-R1 to prevent constitutive activation of the signaling events downstream. It dissociates from the TNF-R1 after TNF binds TNF-R1 to allow the activation of apoptotic pathway. It is expressed ubiquitously in human tissues.

General readings

Annunziata CM, Kleinberg L, Davidson B, Berner A, Gius D, Tchabo N, Steinberg SM, Kohn EC.
BAG-4/SODD and associated antiapoptotic proteins are linked to aggressiveness of epithelial ovarian cancer.
Clin Cancer Res. 2007 Nov 15;13(22 Pt 1):6585-92
PMID: 18006758

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG4 antibody

TA305759 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG4 antibody

Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG4 antibody

Western blot analysis of extracts from K562 cells, using BAG4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG4 antibody

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG4 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BAG4 (C-term) antibody

BAG4 Antibody (C-term) (Cat. #AP50336PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the BAG4 antibody detected the BAG4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BAG4 (N-term) antibody

Immunocytochemical staining of Hela cells with Rabbit anti SODD (SP1301) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

BAG4 (N-term) antibody

Immunocytochemical staining of Hela cells with Rabbit anti SODD (SP1301) Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €280.00
  Acris Antibodies GmbH

BAG4 antibody

Immunoperoxidase of purified MaxPab antibody to BAG4 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

BAG4 antibody

Western Blot analysis of BAG4 expression in transfected 293T cell line (H00009530-T02) by BAG4 MaxPab polyclonal antibody.

Lane 1: BAG4 transfected lysate(49.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

BAG4 antibody

Western Blot analysis of BAG4 expression in transfected 293T cell line by BAG4 monoclonal antibody (M02), clone 2G9.

Lane 1: BAG4 transfected lysate(49.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BAG4 antibody

BAG4 Rabbit Hu C, IP, P
50 µl / €390.00
  Abnova Taiwan Corp.

BAG4 antibody

Western blot analysis of SODD in HeLa cell lysate using BAG4 polyclonal antibody ( Cat # PAB0383 ) at 0.5 µg/ml. Rabbit Bov, Hu, Mky, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

BAG4 antibody

Western blot analysis using BAG4 polyclonal antibody ( Cat # PAB10362 ) on HeLa (lanes 1, 3) and THP-1 (lanes 2, 4) whole cell lysates in the absense (lanes 1, 2) and presense (lanes 3, 4) of blocking peptide. Hu, Ms, Rt
0.1 mg / €470.00
  Abnova Taiwan Corp.

BAG4 antibody

Immunohistochemical analysis of BAG4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BAG4 antibody

Immunohistochemical analysis of BAG4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BAG4 antibody

Immunohistochemical analysis of BAG4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BAG4 antibody

ELISA: BAG4 Antibody [21080002] Rabbit Hu E, P
50 µg / €440.00
  Novus Biologicals Inc.

BAG4 antibody

BAG4 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

BAG4 antibody

Western Blot: SODD Antibody [NBP1-28496]-(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer) Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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