BAG5 antibody

Principal name

BAG5 antibody

Alternative names for BAG5 antibody

BAG-5, KIAA0873, BAG family molecular chaperone regulator 5, Bcl-2-associated athanogene 5

SwissProt ID

Q2TA08 (Bovin), Q5F486 (Chick), Q5QJC9 (Rat), Q8CI32 (Mouse), Q9UL15 (Human), Q9UL15-2

Gene ID

9529 (BAG5), 70369 (Bag5), 366734

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Ms (Mouse), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), EPair for Elisa (EPair)

Background of BAG5 antibody

BAG5 is a member of the BAG1 related protein family. BAG1 is an anti apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL 2, Raf protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. A BAG domain near the C terminus, may bind and inhibit the chaperone activity of Hsc70/Hsp70.
It has been hypothesized that the BAG5 protein will induce the death of nigral neurons through its predicted interaction with hsp70, which will cause increased protein aggregation and cell death by disinhibition of hsp70’s anti apoptotic function. It is believed that BAG5 will play an important role in the mechanisms of neuronal death. BAG5 may also be of interest due to its possible role as a modulator of the hsp70/hsp40 chaperone axis or its possible interaction and coordination of localization/modulation of other BAG containing proteins via BAG-BAG
heterodimerization.

General readings

1 Takayama S, Xie Z, Reed JC (Feb 1999). "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators". J Biol Chem 274 (2): 781–6.
2 Kalia SK, Lee S, Smith PD, Liu L, Crocker SJ, Thorarinsdottir TE, Glover JR, Fon EA, Park DS, Lozano AM (Dec 2004). "BAG5 inhibits parkin and enhances dopaminergic neuron degeneration". Neuron 44 (6): 931–45.
3 "Entrez Gene: BAG5 BCL2-associated athanogene 5"

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG5 (N-term) antibody

AP31064PU-N BAG5 antibody 
staining of nuclear HeLa lysate at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BAG5 antibody

  Goat Anti-BAG5 Antibody  
TA305758 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG5 antibody

  Rabbit polyclonal antibody to BAG5 (BCL2-associated athanogene 5)  
Sample (30 ug of whole cell lysate). A: Hela. B: Molt-4 . 10% SDS PAGE. TA308752 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG5 antibody

  Rabbit polyclonal anti-BAG5 antibody  
Western blot analysis of extracts from HepG2 cells, using BAG5 Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG5 antibody

  Anti-BAG5 Rabbit Polyclonal Antibody  
Predicted band size: 51 kDa . Positive control: A549, Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Hela cell lysate Lane3: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG5 antibody

  Anti-BAG5 Rabbit Polyclonal Antibody  
Predicted band size: 51 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 20 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG5 antibody

BAG5 Rabbit Purified Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

BAG5 antibody

Figure 1. Staining BAG5 in Breast by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BAG5 (N-term) antibody

BAG5 Antibody (N-term) (Cat. #AP50337PU-N) western blot analysis in Jurkat cell line lysates (35µg/lane).This demonstrates the BAG5 antibody detected the BAG5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

BAG5 - human gene knockout kit via CRISPR

BAG5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

BAG5 - human gene knockout kit via CRISPR

BAG5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BAG5 - human gene knockout kit via CRISPR

BAG5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BAG5 - human gene knockout kit via CRISPR

BAG5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bag5 - mouse gene knockout kit via CRISPR

Bag5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Bag5 - mouse gene knockout kit via CRISPR

Bag5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bag5 - mouse gene knockout kit via CRISPR

Bag5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bag5 - mouse gene knockout kit via CRISPR

Bag5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BAG5 (transcript variant 2)

  Recombinant protein of human BCL2-associated athanogene 5 (BAG5), transcript variant 2  
BAG5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BAG5 (transcript variant 3)

  Recombinant protein of human BCL2-associated athanogene 5 (BAG5), transcript variant 3  
BAG5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, BAG5 (mGFP-tagged)-Human BCL2-associated athanogene 5 (BAG5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, BAG5 (mGFP-tagged)-Human BCL2-associated athanogene 5 (BAG5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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