Bamacan antibody

Principal name

Bamacan antibody

Alternative names for Bamacan antibody

Chondroitin sulfate proteoglycan 6, SMC3, BAM, BMH, CSPG6, SMC3L1, hCAP, Structural maintenance of chromosomes protein 3, Chromosome-associated polypeptide, Basement membrane-associated chondroitin proteoglycan

SwissProt ID

O97594 (Bovin), P97690 (Rat), Q803N2, Q9CW03 (Mouse), Q9UQE7 (Human)

Gene ID

9126 (SMC3), 5324475 (A6URM7)

Available reactivities

Hu (Human), Xen (Xenopus), Bov (Bovine), Ms (Mouse), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of Bamacan antibody

SMC3 (UniProtKB/Swiss-Prot entry Q9UQE7) is a central component of the cohesion complex, which is required for chromosome cohesion during the cell cycle. The cohesion complex may form a large proteiceous ring within which sister chromatids can be trapped. At aphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to D replication and is involved in D repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosome movement. Acetylation of SMC3 is required for the establishment of cohesion during cell division.

General readings

Matsuoka et al., Science 316 (5828):1160-1166, 2007.

17 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bamacan antibody

WB Suggested Anti-SMC3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate.There is BioGPS gene expression data showing that SMC3 is expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Bamacan antibody

WB Suggested Anti-SMC3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: COLO205 cell lysate.There is BioGPS gene expression data showing that SMC3 is expressed in COLO205 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Bamacan antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against yeast Smc3K112/113ac. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:16,900. Rabbit IgG Purified Ye E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bamacan antibody

Immunofluorescence of purified MaxPab antibody to CSPG6 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Bamacan antibody

Immunofluorescence of purified MaxPab antibody to CSPG6 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Bamacan antibody

Immunoprecipitation of SMC3 transfected lysate using anti-SMC3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CSPG6 MaxPab mouse polyclonal antibody (B01) (H00009126-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Bamacan antibody

Western Blot analysis of SMC3 expression in transfected 293T cell line by CSPG6 monoclonal antibody (M02), clone 2F11.

Lane 1: SMC3 transfected lysate(141.5 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 2F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Bamacan antibody

Human Testis; Testis; SMC3 antibody - C-terminal region (ARP47590_P050) in Human Testis cells using Immunohistochemistry Rabbit Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Bamacan antibody

Bamacan Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Bamacan antibody

Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - See Notes, IHC Caption B Rabbit Aff - Purified Hu, Xen ICC/IF, IP, P, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Bamacan pSer383 antibody

Immunoprecipitation: SMC3 Phosphospecific Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence. Rabbit Aff - Purified Hu IP
0.1 mg / €490.00
  Novus Biologicals Inc.

Bamacan pSer1083 antibody

Immunohistochemistry-Paraffin: SMC3 [p Ser1083] Antibody [NB100-653] - FFPE serial sections of human colon adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-SMC3. Affinity purified_x000d_
rabbit anti-Phospho-SMC3 (S1083) used at a dilution of 1:250. Detection: DAB staining using anti-rabbit IHC Antibody at a dilution of 1:100. Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €490.00
  Novus Biologicals Inc.

Bamacan antibody

Bamacan Rabbit Agarose Hu, Xen IP
0.1 mg / €400.00
  Novus Biologicals Inc.

Bamacan antibody

Western Blot: SMC3 Antibody [NBP1-19109]- Whole cell lysate from HeLa (5, 15 and 50 mcg), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells.  Affinity purified rabbit anti-SMC3 antibody  used for WB at 0.04 mcg/ml. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Bamacan

Bamacan Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Bamacan

Bamacan Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SMC3 293T Cell Transient Overexpression Lysate(Denatured)

SMC3 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

17 Item(s)

per page
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