BAT8 antibody

Principal name

BAT8 antibody

Alternative names for BAT8 antibody

EHMT2, C6orf30, Protein G9A, KMT1C, NG36, H3-K9-HMTase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Lysine N-methyltransferase 1C

SwissProt ID

Q96KQ7 (Human), Q9Z148 (Mouse)

Gene ID

110147 (Ehmt2), 10919 (EHMT2)

Available reactivities

Ms (Mouse), Can (Canine), Hu (Human), Por (Porcine), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Hamster, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ChIP assay (ChIP)

Background of BAT8 antibody

G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain origilly identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins . Recombint G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro. However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptiolly repressive marks that facilitate gene silencing. G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex. The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability. Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex. In addition, G9a and GLP are components of other large transcriptiol co-repressor complexes, such as those involving E2F6 and CDP/cut. G9a interacts with DNMT1, and both proteins are required for methylation of D and histone H3 (Lys9) at replication foci, providing a functiol link between histone H3 Lys9 and CpG methylation during D replication. G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes. In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAT8 antibody

  EHMT2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using EHMT2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAT8 antibody

  Rabbit Polyclonal Anti-EHMT2 Antibody  
WB Suggested Anti-EHMT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

BAT8 antibody

  Rabbit Polyclonal G9a Antibody  
WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right. Rabbit IgG Purified Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAT8 (621-1000) antibody

Western blot analysis of G9a expression in 22-10(G9a-/-) cells (1), TT2(G9a+/+) cells (2) and HeLa cells (3) using AM26487AF-N Hamster IgG 1-14 Azide Free Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BAT8 antibody

Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) cells with Ehmt2 polyclonal antibody ( Cat # PAB14063 ), diluted 1 : 1,000 in BSA/PBS-Tween. Rabbit Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

BAT8 antibody

Human Muscle; WB Suggested Anti-EHMT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; EHMT2 antibody - N-terminal region (ARP32493_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Por WB
50 µg / €350.00
  AVIVA Systems Biology

BAT8 antibody

BAT8 Mouse IgG2a A8620A Purified Hu, Ms ELISA, IHC, IP, WB
0.1 ml / €640.00
  Funakoshi Co., Ltd.

CRISPR

Catalog No. Species Donor Vector  

EHMT2 - human gene knockout kit via CRISPR

EHMT2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EHMT2 - human gene knockout kit via CRISPR

EHMT2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EHMT2 - human gene knockout kit via CRISPR

EHMT2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EHMT2 - human gene knockout kit via CRISPR

EHMT2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ehmt2 - mouse gene knockout kit via CRISPR

Ehmt2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ehmt2 - mouse gene knockout kit via CRISPR

Ehmt2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ehmt2 - mouse gene knockout kit via CRISPR

Ehmt2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ehmt2 - mouse gene knockout kit via CRISPR

Ehmt2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BAT8 (transcript variant NG36/G9a-SPI)

  Recombinant protein of human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a-SPI  
BAT8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

EHMT2 overexpression lysate

EHMT2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EHMT2 (mGFP-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a, 200 uL, >10^7 TU/mL

Lenti ORF particles, EHMT2 (mGFP-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a, 200 uL, >10^7 TU/mL  
200 µl / €2,370.00
  OriGene Technologies, Inc.

Lenti ORF particles, EHMT2 (mGFP-tagged) - Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a-SPI, 200 uL, >10^7 TU/mL

Lenti ORF particles, EHMT2 (mGFP-tagged) - Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a-SPI, 200 uL, >10^7 TU/mL  
200 µl / €2,330.00
  OriGene Technologies, Inc.

Lenti ORF particles, EHMT2 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a, 200 uL, >10^7 TU/mL

Lenti ORF particles, EHMT2 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a, 200 uL, >10^7 TU/mL  
200 µl / €2,140.00
  OriGene Technologies, Inc.

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