Bax antibody

Principal name

Bax antibody

Alternative names for Bax antibody

Apoptosis regulator BAX, BCL2L4, Bcl2-L-4, Bcl-2-like protein 4, BCL2-associated X protein

SwissProt ID

O02703 (Bovin), Q07812 (Human), Q07813 (Mouse), Q63690 (Rat)

Gene ID

581 (BAX), 12028 (Bax)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Rb (Rabbit), Por (Porcine)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of Bax antibody

The human protein Bax sits at a critical regulatory junction of apoptosis, or programmed cell death. Activated Bax changes conformation, inserts into the MOM (Mitochondrial Outer Membrane), oligomerizes, and induces MOM permeabilization, causing the release of cytochrome c, which effectively commits the cell to die. (Ma J et al., 2012). Mechanisms of membrane perforation include formation of hetero-oligomeric complexes of Bax with other pro-apoptotic proteins such as Bak, or formation of lipidic pores physically aided by mitochondrial membrane-inserted proteins (Garg P et al., 2012). Connexins play important roles in many physiological and pathological processes. In the context of apoptosis, Cx43 translocated to the mitochondria, where it interacted with Bax to initiate the mitochondrial apoptotic pathway. The 241-382 aa region of Cx43 was required for interaction with Bax. Furthermore, this region was responsible for permeabilizing mitochondrial membrane potential. Recent studies elucidate a novel mechanism of the Cx43-mediated regulation of apoptosis in pancreatic cancer (Sun Y et al., 2012). 
Bax acts as a biomarker that exhibited a difference in sub-cellular localization between normal OCSE (Oral Cavity Squamous Epithelium) and OSCC (Oral Cavity Squamous Cell Carcinoma) and was also the only apoptotic protein significantly associated with prognosis. The translocation of Bax from the nucleus to the cytoplasm in OSCC is consistent with increased Bax function at the mitochondria, leading to improved sensitivity to radiotherapy-induced apoptosis in tumours with elevated Bax expression. Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signaling (Bose P et al., 2012).

General readings

1. Dinh C  et al. Short Interfering RNA against Bax Attenuates TNFa-Induced Ototoxicity in Rat Organ of Corti Explants. Otolaryngol Head Neck Surg 148:834-40 (2013). ICC/IF ; Rat . 
2. Monsalve DM  et al. Human VRK2 modulates apoptosis by interaction with Bcl-xL and regulation of BAX gene expression. Cell Death Dis 4:e513 (2013). Human . 

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Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Bax (transcript variant alpha)

  Purified recombinant protein of Homo sapiens BCL2-associated X protein (BAX), transcript variant alpha  
Bax Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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