BBS4 antibody

Principal name

BBS4 antibody

Alternative names for BBS4 antibody

Bardet-Biedl syndrome 4 protein

SwissProt ID

Q1JQ97 (Bovin), Q8C1Z7 (Mouse), Q96RK4 (Human)

Gene ID

585 (BBS4), 102774 (Bbs4)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Bov (Bovine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of BBS4 antibody

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BBS4 (33-46) antibody

AP23221PU-N BBS4 antibody staining of Human Testis lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BBS4 (125-139) antibody

  Goat Anti-BBS4 (aa 125-139) Antibody  
(1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BBS4 antibody

  Mouse monoclonal anti-BBS4 antibody (Center)  
Western blot analysis of lysates from Hela, U251 cell line, mouse heart and kidney, rat heart and kidney tissue (from left to right), using PHB Antibody (Cat. #TA328137). TA328137 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Mouse IgG1 1292CT845.130.218 Purified Hu, Ms, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

BBS4 antibody

  Rabbit Polyclonal Anti-BBS4 Antibody - middle region  
WB Suggested Anti-BBS4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

BBS4 antibody

  Rabbit Polyclonal Anti-BBS4 Antibody - N-terminal region  
WB Suggested Anti-BBS4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

BBS4 antibody

  BBS4 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBS4 (RC206210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBS4. Mouse IgG1 OTI2D5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BBS4 (Center) antibody

Western blot analysis of anti-BBS4 Pab in Hela cell line lysates (35ug/lane).BBS4 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BBS4 antibody

Western Blot analysis of BBS4 expression in transfected 293T cell line (H00000585-T01) by BBS4 MaxPab polyclonal antibody.

Lane 1: BBS4 transfected lysate(57.09 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BBS4 antibody

Western Blot analysis of BBS4 expression in transfected 293T cell line (H00000585-T01) by BBS4 MaxPab polyclonal antibody.

Lane 1: BBS4 transfected lysate(57.09 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

BBS4 antibody

BBS4 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

BBS4 antibody

BBS4 Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BBS4 antibody

Western blot analysis of BBS4 polyclonal antibody ( Cat # PAB2963 ) in Hela cell line lysates ( 35 µg/lane ) .BBS4 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

BBS4 (N-term) antibody

Human HepG2; WB Suggested Anti-BBS4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; BBS4 antibody - N-terminal region (ARP57695_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

BBS4 (Center) antibody

Human HepG2; WB Suggested Anti-BBS4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; BBS4 antibody - middle region (ARP57696_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

BBS4 - human gene knockout kit via CRISPR

BBS4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

BBS4 - human gene knockout kit via CRISPR

BBS4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BBS4 - human gene knockout kit via CRISPR

BBS4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BBS4 - human gene knockout kit via CRISPR

BBS4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bbs4 - mouse gene knockout kit via CRISPR

Bbs4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Bbs4 - mouse gene knockout kit via CRISPR

Bbs4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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