BCAR3 / SH2D3B antibody

Principal name

BCAR3 / SH2D3B antibody

Alternative names for BCAR3 / SH2D3B antibody

NSP2, Breast cancer anti-estrogen resistance protein 3, SH2 domain-containing protein 3B, Novel SH2-containing protein 2

SwissProt ID

O75815 (Human), Q58DL5 (Bovin), Q9QZK2 (Mouse)

Gene ID

8412 (BCAR3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BCAR3 / SH2D3B antibody

In breast cancer, the antiestrogen tamoxifen has been prescribed for both primary treatment and the treatment of advanced metastatic disease. Although the drug induces remission in most patients with estrogen receptor-positive disease, all patients eventually develop resistance. van Agthoven et al. (1998) applied an insertional mutagenesis strategy to a breast cancer cell line. Infected cells were subjected to tamoxifen selection, and the resistant clones were screened for a common integration site linked with antiestrogen resistance. By screening a human testis cDNA library with the integration site-specific probe, they obtained a cDNA encoding BCAR3 (breast cancer antiestrogen resistance 3). The deduced 825 amino acid BCAR3 protein contains a putative Src homology 2 (SH2) domain and sequences homologous to yeast CDC48. Northern blot analysis detected abundant expression of a 3.4kb BCAR3 transcript in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and several cancer cell lines, but not in nonmalignant breast tissue; a 6kb BCAR3 transcript was also detected in skeletal muscle and heart.

General readings

van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCAR3 / SH2D3B antibody

TA305647 (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Ms, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B antibody

Western blot analysis of extracts from HeLa cells, Jurkat cells and HUVEC cells, using BCAR3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B (N-term) antibody

Western blot analysis of BCAR3 in mouse kidney tissue lysate with BCAR3 antibody at A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B antibody

Western blot analysis on HeLa cell lysate using BCAR3 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B antibody

ELISA: BCAR3 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B (N-term) Control Peptide

BCAR3 / SH2D3B
50 µg / €160.00
  Acris Antibodies GmbH

BCAR3 / SH2D3B antibody

Western Blot analysis of BCAR3 expression in transfected 293T cell line by BCAR3 polyclonal antibody (A01).

Lane1:BCAR3 transfected lysate(93 KDa).
Lane2:Non-transfected lysate. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

BCAR3 / SH2D3B antibody

Detection limit for recombinant GST tagged BCAR3 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 3G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BCAR3 / SH2D3B antibody

BCAR3 / SH2D3B Goat Ms E
0.1 mg / €400.00
  Abnova Taiwan Corp.

BCAR3 / SH2D3B antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using BCAR3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €205.00
  Assay Biotech

BCAR3 / SH2D3B antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using BCAR3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €310.00
  Assay Biotech

BCAR3 / SH2D3B antibody

ELISA: BCAR3 Antibody [26000002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

BCAR3 / SH2D3B antibody

Western Blot and  Immunoprecipitation: BCAR3 Antibody [NB100-87003] - Detection of Human BCAR3 on HeLa whole cell lysate using NB100-87003. BCAR3 was also immunoprecipitated by another rabbit anti-BCAR3 antibody. Rabbit Aff - Purified Hu, Rt IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

BCAR3 / SH2D3B antibody

Western Blot: BCAR3 Antibody [NB300-825]- (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer) Goat IgG Aff - Purified Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

BCAR3 / SH2D3B antibody

BCAR3 / SH2D3B Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

BCAR3 / SH2D3B antibody

BCAR3 / SH2D3B Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

BCAR3 / SH2D3B antibody

Immunohistochemistry-Paraffin: BCAR3 Antibody [NBP1-82523] - Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BCAR3 / SH2D3B

BCAR3 / SH2D3B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BCAR3 / SH2D3B

BCAR3 / SH2D3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

BCAR3 / SH2D3B

BCAR3 / SH2D3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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