BCAR3 / SH2D3B antibody

Principal name

BCAR3 / SH2D3B antibody

Alternative names for BCAR3 / SH2D3B antibody

NSP2, Breast cancer anti-estrogen resistance protein 3, SH2 domain-containing protein 3B, Novel SH2-containing protein 2

SwissProt ID

O75815 (Human), Q58DL5 (Bovin), Q9QZK2 (Mouse)

Gene ID

8412 (BCAR3), 29815 (Bcar3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BCAR3 / SH2D3B antibody

In breast cancer, the antiestrogen tamoxifen has been prescribed for both primary treatment and the treatment of advanced metastatic disease. Although the drug induces remission in most patients with estrogen receptor-positive disease, all patients eventually develop resistance. van Agthoven et al. (1998) applied an insertiol mutagenesis strategy to a breast cancer cell line. Infected cells were subjected to tamoxifen selection, and the resistant clones were screened for a common integration site linked with antiestrogen resistance. By screening a human testis cD library with the integration site-specific probe, they obtained a cD encoding BCAR3 (breast cancer antiestrogen resistance 3). The deduced 825 amino acid BCAR3 protein contains a putative Src homology 2 (SH2) domain and sequences homologous to yeast CDC48. Northern blot alysis detected abundant expression of a 3.4kb BCAR3 transcript in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and several cancer cell lines, but not in nonmalignt breast tissue; a 6kb BCAR3 transcript was also detected in skeletal muscle and heart.

General readings

van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808.

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCAR3 / SH2D3B antibody

  Goat Anti-BCAR3 Antibody  
TA305647 (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Ms, Por WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B (N-term) antibody

  Rabbit Polyclonal BCAR3 Antibody  
Western blot analysis of BCAR3 in mouse kidney tissue lysate with BCAR3 antibody at A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

BCAR3 / SH2D3B antibody

  Rabbit Polyclonal Anti-BCAR3 Antibody  
Western blot analysis on HeLa cell lysate using BCAR3 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

3 Item(s)

per page
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