BCAS2 / DAM1 antibody

Principal name

BCAS2 / DAM1 antibody

Alternative names for BCAS2 / DAM1 antibody

SPF27, Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein

SwissProt ID

O75934 (Human), Q9D287 (Mouse)

Gene ID

10286 (BCAS2)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Xen (Xenopus), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of BCAS2 / DAM1 antibody

BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TR' (thyroid hormone receptor '), ER' (estrogen receptor '), ER', PR (progesterone receptor) and PPAR' (peroxisome proliferator-activated receptor '). This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer. Recombinant human BCAS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Qi C., et al. (2005) Biochem Biophys Res Commun. 328(2):393-8.
Kuo PC., et al. (2009) Cancer Res. 69(23):8877-85.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCAS2 / DAM1 antibody

Western blot analysis of BCAS2 over-expressed 293 cell line, cotransfected with BCAS2 Validated Chimera RNAi ( Cat # H00010286-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BCAS2 monoclonal antibody (M01), clone 1D10 (Cat # H00010286-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2a 1D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BCAS2 / DAM1 antibody

Western blot analysis of BCAS2 in MCF7 cell lysate with BCAS2 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCAS2 / DAM1 antibody

Western blot analysis of extracts from HeLa cells, HepG2 cells, Huvec cells, A549 cells, K562 cells and Jurkat cells, using BCAS2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCAS2 / DAM1 antibody

WB Suggested Anti-BCAS2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

BCAS2 / DAM1 antibody

WB Suggested Anti-BCAS2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

BCAS2 / DAM1 antibody

ELISA: BCAS2 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCAS2 / DAM1 (C-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BCAS2 / DAM1 Control Peptide

BCAS2 / DAM1
50 µg / €160.00
  Acris Antibodies GmbH

BCAS2 / DAM1 antibody

BCAS2 polyclonal antibody (A01), Lot # 051214JC01 Western Blot analysis of BCAS2 expression in U-2 OS ( Cat # L022V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

BCAS2 / DAM1 antibody

Immunofluorescence of purified MaxPab antibody to BCAS2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

BCAS2 / DAM1 antibody

BCAS2 / DAM1 Mouse IgG2a 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BCAS2 / DAM1 antibody

Human Liver; WB Suggested Anti-BCAS2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; BCAS2 antibody - middle region (ARP40613_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

BCAS2 / DAM1 antibody

Human Brain; WB Suggested Anti-BCAS2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; BCAS2 antibody - N-terminal region (ARP40614_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

BCAS2 / DAM1 antibody

Western blot analysis of BCAS2 expression in MCF7 (A); A549 (B); SHSY5Y (C); PC12 (D); NIH3T3 (E) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BCAS2 / DAM1 antibody

Western blot analysis of BCAS2 expression in MCF7 (A); A549 (B); SHSY5Y (C); PC12 (D); NIH3T3 (E) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BCAS2 / DAM1 antibody

Western blot analysis of BCAS2 expression in MCF7 (A); A549 (B); SHSY5Y (C); PC12 (D); NIH3T3 (E) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BCAS2 / DAM1 antibody

ELISA: BCAS2 Antibody [26010002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

BCAS2 / DAM1 antibody

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Section of mouse squamous cell carcinoma.  Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

BCAS2 / DAM1 antibody

Immunohistochemistry-Paraffin: BCAS2 Antibody [NBP1-77124] - Human breast carcinoma with BCAS2 antibody at 5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BCAS2 / DAM1

BCAS2 / DAM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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