BCAT1 antibody

Principal name

BCAT1 antibody

Alternative names for BCAT1 antibody

BCT1, ECA39, BCAT(c), Branched-chain-amino-acid aminotransferase cytosolic

SwissProt ID

P24288 (Mouse), P54687 (Human), P54690 (Rat), Q9GKM4 (Sheep)

Gene ID

586 (BCAT1), 12035 (Bcat1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of BCAT1 antibody

BCAT1,Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors.The BCAT1 gene is mapped to chromosome 12 .Lack of the enzyme BCT can cause auxotroph ,a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

General readings

Goto M., et al. (2005) J. Biol. Chem. 280:37246-37256Schuldiner O., et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93:7143-7148

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCAT1 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504360) Mouse IgG2a OTI3F5 Purified Can, Hu, Ms P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

BCAT1 antibody

WB Suggested Anti-BCAT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Small Intestine Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

BCAT1 antibody

WB Suggested Anti-Bcat1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

BCAT1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BCAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : Jurkat cell lysate 
Lane 2. : HeLa cell lysate Mouse IgG1 AT3C8 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

BCAT1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BCAT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : Jurkat cell lysate 
Lane 2. : HeLa cell lysate Mouse IgG1 AT3C8 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

BCAT1 (Center) antibody

BCAT1 Antibody (Center) (Cat. #AP50350PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCAT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BCAT1 antibody

Western Blot analysis of BCAT1 expression in transfected 293T cell line (H00000586-T01) by BCAT1 MaxPab polyclonal antibody.

Lane 1: BCAT1 transfected lysate(35.31 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

BCAT1 antibody

BCAT1 Mouse IgG2a 1F8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

BCAT1 antibody

Human Small Intestine; WB Suggested Anti-BCAT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Small Intestine; BCAT1 antibody - N-terminal region (ARP46132_P050) in Human Small Intestine cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

BCAT1 antibody

BCAT1 Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

BCAT1 antibody

Western blot analysis of ECA39 expression in Jurkat (A); Ramos (B); NIH3T3 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BCAT1 antibody

Western blot analysis of ECA39 expression in Jurkat (A); Ramos (B); NIH3T3 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BCAT1 antibody

Western blot analysis of ECA39 expression in Jurkat (A); Ramos (B); NIH3T3 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BCAT1 antibody

BCAT1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BCAT1

BCAT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BCAT1 (full length, N-term HIS tag, transcript variant 1)

BCAT1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

BCAT1 (1-386, His-tag)

BCAT1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

BCAT1 (1-386, His-tag)

BCAT1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

BCAT1 Lysate(Denatured)

BCAT1 Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

BCAT1 overexpression lysate

BCAT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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