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BCKDHB antibody

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Principal name

BCKDHB antibody

Alternative names for BCKDHB antibody

alpha-keto acid dehydrogenase, 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial Branched-chain alpha-keto acid dehydrogenase E1 component beta chain

SwissProt ID

P21839 (Bovin), P21953 (Human), P35738 (Rat), Q6P3A8 (Mouse)

Gene ID

594 (BCKDHB)

Ncbi ID

9606, NP_000047, NP_000047.1, NM_000056, NM_183050, NP_898871

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of BCKDHB antibody

Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems. Alternative splicing at this locus results in transcript variants with different 3' non-coding regions, but encoding the same isoform. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

NBL1-07943 - BCKDHB Lysate
Western Blot: BCKDHB Lysate [NBL1-07943] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BCKDHB
NBL1-07944 - BCKDHB Lysate
Western Blot: BCKDHB Lysate [NBL1-07944]
AP50353PU-N - BCKDHB
BCKDHB Antibody (N-term) (Cat. #AP50353PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
AP50353PU-N - BCKDHB
Confocal immunofluorescent analysis of BCKDHB Antibody (N-term)(Cat#AP50353PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
AP50353PU-N - BCKDHB
BCKDHB Antibody (N-term) (Cat. #AP50353PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).
AP50353PU-N - BCKDHB
Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term) (Cat. #AP50353PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.
AP50353PU-N - BCKDHB
BCKDHB Antibody (N-term) (Cat. #AP50353PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NBP1-86326 - BCKDHB
Immunohistochemistry-Paraffin: BCKDHB Antibody [NBP1-86326] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity(granular pattern) in glandular cells.
NBP1-86326 - BCKDHB
Western Blot: BCKDHB Antibody [NBP1-86326] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
13685-1-AP - BCKDHB
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 13685-1-AP( BCKDHB Antibody) at dilution of 1:50 (under 40x lens)
13685-1-AP - BCKDHB
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13685-1-AP( BCKDHB Antibody) at dilution of 1:50 (under 10x lens)
13685-1-AP - BCKDHB
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 13685-1-AP( BCKDHB Antibody) at dilution of 1:50 (under 10x lens)
13685-1-AP - BCKDHB
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13685-1-AP( BCKDHB Antibody) at dilution of 1:50 (under 40x lens)
13685-1-AP - BCKDHB
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP( BCKDHB Antibody) at dilution of 1:600
13685-1-AP - BCKDHB
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP( BCKDHB Antibody) at dilution of 1:350
TP308059 - BCKDHB
  1. Quick-Filters:
  2. Highly Validated (1)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCKDHB (N-term) antibody
BCKDHB Antibody (N-term) (Cat. #TA324362) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

BCKDHB antibody
Host: Rabbit; Target Name: BCKDHB; Sample Tissue: Thyroid tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

BCKDHB antibody
Immunohistochemistry-Paraffin: BCKDHB Antibody [NBP1-86326] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity(granular pattern) in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BCKDHB (transcript variant 1)
BCKDHB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BCKDHB
BCKDHB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

BCKDHB overexpression lysate
BCKDHB overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

BCKDHB overexpression lysate
BCKDHB overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

7 Item(s)

per page

Available Filters

Product Type
  1. Lysate (2)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (3)
Target
  1. BCKDHB (5)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (3)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (2)
  3. Flow cytometry (F) (1)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (4)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
  4. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (1)
  2. in vitro transl. (1)
Manufacturer
  1. Acris - OriGene (5)
  2. Abnova Taiwan Corp. (1)
  3. Novus Biologicals Inc. (1)
Service
  • OriGene EU
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