BCKDHB antibody
Principal name
BCKDHB antibody
Alternative names for BCKDHB antibody
alpha-keto acid dehydrogenase, 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
Ncbi ID
NM_000056, NM_183050, NP_898871, 9606, NP_000047, NP_000047.1, NP_954665.1, NM_199195
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of BCKDHB antibody
- Kit Component:
- - KN302091G1, Bckdhb gRNA vector 1 in pCas-Guide vector
- KN302091G2, Bckdhb gRNA vector 2 in pCas-Guide vector
- KN302091D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
BCKDHB Antibody (N-term) (Cat. #TA324362) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).
Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term) (Cat. #TA324362). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.
BCKDHB Antibody (N-term) (Cat. #TA324362) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of BCKDHB Antibody (N-term)(Cat#TA324362) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
BCKDHB Antibody (N-term) (Cat. #TA324362) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Host: Rabbit; Target Name: BCKDHB; Sample Tissue: Thyroid tumor lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324362 | BCKDHB (N-term) antibody |
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| Rabbit polyclonal BCKDHB Antibody (N-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA344275 | BCKDHB antibody |
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| Rabbit Polyclonal Anti-BCKDHB Antibody - N-terminal region | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN208059 | BCKDHB - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208059BN | BCKDHB - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208059LP | BCKDHB - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208059RB | BCKDHB - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302091 | Bckdhb - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302091BN | Bckdhb - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302091LP | Bckdhb - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN302091RB | Bckdhb - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308059 | BCKDHB (transcript variant 1) |
||||
| Recombinant protein of human branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), nuclear gene encoding mitochondrial protein, transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400014 | BCKDHB overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY405219 | BCKDHB overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204658L2V | Lenti ORF particles, Bckdhb (GFP-tagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR204658L1V | Lenti ORF particles, Bckdhb (Myc-DDK-tagged) - Mouse branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
