BCKDK antibody

Principal name

BCKDK antibody

Alternative names for BCKDK antibody

BCKD-kinase, BCKDHKIN, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase

SwissProt ID

O14874 (Human), O55028 (Mouse), Q00972 (Rat), Q2KJG8 (Bovin)

Gene ID

10295 (BCKDK)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Mky (Monkey), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of BCKDK antibody

The second major step in the catabolism of the branched-chain amino acids, isoleucine, leucine, and valine, is irreversibly catalyzed by the branched-chain alpha-keto acid dehydrogenase complex (BCKD), an inner-mitochondrial enzyme complex composed of 3 catalytic components: a branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). The complex also contains 2 enzymes that regulated the state of activity of the BCKD complex: a kinase (BCKDK), and a phosphorylase. The ubiquitiously expressed kinase contains 1 histidine kinase domain. Maple syrup urine disease (MSUD) is a pathology secondary to an enzyme defect in the catabolic pathway of leucine, isoleucine, and valine. Accumulation of these amino acids and their corresponding keto acids results in encephalopathy and progressive neurodegeneration in infants not treated for MSUD.

General readings

Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCKDK antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BCKDK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500685) Mouse IgG1 OTI11C9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

BCKDK (Center) antibody

Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (Center) (Cat.#TA302079). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCKDK antibody

Western blot analysis of extracts from 293 cells and Jurkat cells, using BCKD antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCKDK antibody

WB Suggested Anti-BCKDK Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

BCKDK antibody

Western blot analysis on Jurkat and K562 cell lysate using BCKD Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BCKDK (C-term) antibody

(LEFT)The anti-BCKDK Pab is used in Western blot to detect BCKDK in mouse intestine tissue lysate (Lane 1) and Hela cell lysate (Lane 2). (RIGHT)Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BCKDK antibody

BCKDK Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

BCKDK antibody

BCKDK Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

BCKDK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BCKDK polyclonal antibody ( Cat # PAB2655 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BCKDK antibody

The BCKDK polyclonal antibody ( Cat # PAB4652 ) is used in Western blot to detect BCKDK in mouse intestine tissue lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

BCKDK antibody

Immunofluorescence analysis of HeLa cells, using BCKD Antibody . The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

BCKDK antibody

Immunofluorescence analysis of HeLa cells, using BCKD Antibody . The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

BCKDK antibody

Human Brain; WB Suggested Anti-BCKDK Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; BCKDK antibody - N-terminal region (ARP52131_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

BCKDK antibody

Immunohistochemical analysis of BCKDK staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BCKDK antibody

Immunohistochemical analysis of BCKDK staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BCKDK antibody

Immunohistochemical analysis of BCKDK staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BCKDK antibody

Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BCKDK mouse monoclonal antibody. (Dilution 1:50) Mouse 11C9 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

BCKDK antibody

Western Blot: BCKDK Antibody [NBP1-57574] - This Anti-BCKDK antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BCKDK

BCKDK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BCKDK

BCKDK Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

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