Bcl-2-like 2 antibody

Principal name

Bcl-2-like 2 antibody

Alternative names for Bcl-2-like 2 antibody

Bcl2-L-2, BCL2L2, BCLW, Bcl-w, KIAA0271

SwissProt ID

P70345 (Mouse), Q1RMX3 (Bovin), Q45T69 (Canfa), Q92843 (Human)

Gene ID

599 (BCL2L2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Mky (Monkey), Rt (Rat), Chk (Chicken), Can (Canine)

Available hosts

Mouse, Goat, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Dot blot (Dot), Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Bcl-2-like 2 antibody

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.

General readings

Zhu X., et al. (2004) J Neurochem. 89(5):1233-40.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bcl-2-like 2 (BH3 Domain) antibody

Western blot analysis of anti-Bcl-w BH3 domain Pab (Cat. #TA302168) in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Western blot analysis of extracts from COLO cells, using BCLW antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Predicted cell location: Cytoplasm. Positive control: Human gastric and liver cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using BCL2L2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Western blot analysis of HL-60 cell lysate using BCL2L2 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Western blot (WB) analysis of Bcl-w antibody (Cat.-No.: AP20550PU-N) in extracts from COLO cell Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Bcl-2-like 2 antibody

Bcl-2-like 2 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

Western Blot analysis of BCL2L2 expression in transfected 293T cell line (H00000599-T02) by BCL2L2 MaxPab polyclonal antibody.

Lane 1: BCL2L2 transfected lysate(21.23 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

Bcl-2-like 2 Mouse IgG2a 2E4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

IP/Western blot showing the detection of BCL2L2 in human and mouse cell lines. Lysates from 107 cells were immunoprecipitated by clone 13F9 and the fractionated proteins were then blotted with BCL2L2 monoclonal antibody, clone 13F9 ( Cat # MAB3487 ). Hsp-70 expression was used as an equal loading control and 293T cells transiently transfected with a human bcl-w expression construct were used as a positive control. Rat IgG2a 13F9 Hu, Mky, Ms, Rt IP
0.1 mg / €640.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

Immunofluorescence analysis of HepG2 cells, using BCLW Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Bcl-2-like 2 antibody

Immunofluorescence analysis of HepG2 cells, using BCLW Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Bcl-2-like 2 antibody

Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Bcl-2-like 2 antibody

Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Bcl-2-like 2 antibody

Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Bcl-2-like 2 antibody

Bcl-w staining of  paraffin-embedded human spleen tissue sections. Tissues were stained using  HRP-DAB (brown) and counterstained with hematoxylin (blue). Goat IgG Aff - Purified Hu, Ms C
0.1 mg / €450.00
  Neuromics Antibodies

Bcl-2-like 2 antibody

Western Blot: Bcl2L2 Antibody [NBP1-19705] - (WB) analysis of Bcl-w (G154) antibody in extracts from COLO cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Bcl-2-like 2 antibody

Bcl-2-like 2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Bcl-2-like 2 antibody

Bcl-2-like 2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Bcl-2-like 2 antibody

Bcl-2-like 2 Rabbit Purified Hu, Ms E, F, IP, P, WB
0.1 mg / €530.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Bcl-2-like 2

Bcl-2-like 2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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