Bcl-2-like 2 antibody

Principal name

Bcl-2-like 2 antibody

Alternative names for Bcl-2-like 2 antibody

Bcl2-L-2, BCL2L2, BCLW, Bcl-w, KIAA0271

SwissProt ID

P70345 (Mouse), Q1RMX3 (Bovin), Q45T69 (Canfa), Q92843 (Human)

Gene ID

599 (BCL2L2), 12050 (Bcl2l2)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Goat, Rat, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Bcl-2-like 2 antibody

The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiological conditions. Bcl-2, A1 and Bcl-xL function to inhibit apoptosis while other members of the Bcl-2 family, Bax, Bad, Bak and Bcl-xS oppose their death-suppressing effects. Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRalpha gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The “rox” portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.

General readings

Zhu X., et al. (2004) J Neurochem. 89(5):1233-40.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bcl-2-like 2 (BH3 Domain) antibody

Western blot analysis of anti-Bcl-w BH3 domain Pab (Cat. #TA302168) in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Western blot analysis of extracts from COLO cells, using BCLW antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Predicted cell location: Cytoplasm. Positive control: Human gastric and liver cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using BCL2L2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Western blot analysis of HL-60 cell lysate using BCL2L2 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

WB Suggested Anti-Bcl2l2 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Testis Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

Bcl-2-like 2 antibody

Western blot (WB) analysis of Bcl-w antibody (Cat.-No.: AP20550PU-N) in extracts from COLO cell Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Bcl-2-like 2 antibody

Bcl-2-like 2 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

Western Blot analysis of BCL2L2 expression in transfected 293T cell line (H00000599-T02) by BCL2L2 MaxPab polyclonal antibody.

Lane 1: BCL2L2 transfected lysate(21.23 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

Bcl-2-like 2 Mouse IgG2a 2E4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

IP/Western blot showing the detection of BCL2L2 in human and mouse cell lines. Lysates from 107 cells were immunoprecipitated by clone 13F9 and the fractionated proteins were then blotted with BCL2L2 monoclonal antibody, clone 13F9 ( Cat # MAB3487 ). Hsp-70 expression was used as an equal loading control and 293T cells transiently transfected with a human bcl-w expression construct were used as a positive control. Rat IgG2a 13F9 Hu, Mky, Ms, Rt IP
0.1 mg / €640.00
  Abnova Taiwan Corp.

Bcl-2-like 2 antibody

Immunofluorescence analysis of HepG2 cells, using BCLW Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Bcl-2-like 2 antibody

Immunofluorescence analysis of HepG2 cells, using BCLW Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Bcl-2-like 2 antibody

Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Bcl-2-like 2 antibody

Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Bcl-2-like 2 antibody

Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Bcl-2-like 2 antibody

Bcl-w staining of  paraffin-embedded human spleen tissue sections. Tissues were stained using  HRP-DAB (brown) and counterstained with hematoxylin (blue). Goat IgG Aff - Purified Hu, Ms C
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

BCL2L2 - human gene knockout kit via CRISPR

BCL2L2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

BCL2L2 - human gene knockout kit via CRISPR

BCL2L2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BCL2L2 - human gene knockout kit via CRISPR

BCL2L2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BCL2L2 - human gene knockout kit via CRISPR

BCL2L2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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