BCR antibody

Principal name

BCR antibody

Alternative names for BCR antibody

Breakpoint cluster region protein, BCR1, D22S11, NY-REN-26, Renal carcinoma antigen NY-REN-26

SwissProt ID

P11274 (Human), Q6PAJ1 (Mouse)

Gene ID

613 (BCR)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Frozen Sections (C)

Background of BCR antibody

The Bcr gene, mapping on chromosome 22, was initially identified on the basis of its fusion with the c-Abl proto-oncogene on chromosome 9 resulting in the generation of the Philadelphia chromosome in 90-95% of patients with chronic myelogenous leukemia (CML). The Bcr gene encodes for the Breakpoint cluster region protein (Bcr). A consequence of this translocation is the generation of a Bcr/c-Abl mRNA encoding an activated c-Abl protein kinase. The Bcr gene has been shown to encode a 160 kDa GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, Rac 1 p21. While it has been speculated that the Bcr protein may also stimulate Rac 2 p21 GTPase activity, it has no effect on Ras p21 or Rho p21 GTPases. It is of interest that the GAP domain of Bcr maps outside of the region that remains on chromosome 22 (Philadelphia chromosome) in CML.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BCR antibody

Immunocytochemistry: Bcr Antibody [NBP1-19097] - FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene)._x000d_
 Affinity purified rabbit anti-BCR used at a dilution of 1:250. Detection: Redfluorescent_x000d_
goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100. Rabbit IgG Aff - Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

BCR antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using BCR antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCR antibody

Western blot analysis of extracts from K562 cells, using Bcr (Ab-177) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCR antibody

Predicted band size: 130; 160; 210 kDa . Positive control: HT-29, RAW264.7, Jurkat cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1-3: HT-29, RAW264.7, Jurkat cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCR antibody

Western blot analysis of Bcr expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCR antibody

Western blot analysis of Bcr expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BCR pTyr177 antibody

Western blot (WB) analysis of p-Bcr (pTyr177) antibody Cat-No.: AP01539PU-N  in extracts from 293 cells treated with Forskolin Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BCR pTyr177 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue, using Bcr antibody (phospho-Tyr177) AP02468PU Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

BCR pTyr177 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue, using Bcr antibody (phospho-Tyr177) AP02468PU Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

BCR antibody

Immunofluorescence staining of methanol-fixed HeLa cells using BCR antibody AP02709PU (Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

BCR antibody

Immunofluorescence staining of methanol-fixed HeLa cells using BCR antibody AP02709PU (Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

BCR antibody

Western blot (WB) analysis of Bcr antibody (Cat.-No.: AP06030PU-N) at 1/500 dilution Lane1:HepG2 cell lysate Lane2:Mouse brain tissue lysate Lane3:Rat brain tissue lysate Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BCR antibody

Immunohistochemistry (IHC) analyzes of Bcr antibody (Cat.-No.: AP06449PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

BCR pTyr360 antibody

Western blot analysis of extracts from COS7 tissue using Bcr (Phospho-Tyr360) antibody AP55922PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

BCR pTyr360 antibody

Western blot analysis of extracts from COS7 tissue using Bcr (Phospho-Tyr360) antibody AP55922PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

BCR antibody

Western Blot analysis of BCR expression in transfected 293T cell line (H00000613-T01) by BCR MaxPab polyclonal antibody.

Lane 1: BCR transfected lysate(118.36 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BCR antibody

BCR Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

BCR antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BCR and PIK3CG HeLa cells were stained with anti-BCR rabbit purified polyclonal 1:1200 and anti-PIK3CG mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BCR antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and BCR. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-BCR mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BCR antibody

Western blot analysis of extracts from A-431 cells, untreated or EGF-treated ( 200 ng/ml, 30 min ), using BCR polyclonal antibody ( Cat # PAB5270 ) . Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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