BDNF antibody

Principal name

BDNF antibody

Alternative names for BDNF antibody

Brain-derived neurotrophic factor, Abrineurin

SwissProt ID

P14082 (Pig), P21237 (Mouse), P23363 (Rat), P23560 (Human), P25429 (Chick), Q0EAB7 (Horse), Q95106 (Bovin)

Gene ID

627 (BDNF), 24225 (Bdnf), 12064

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine)

Available hosts

Chicken, Rabbit, Mouse, Goat, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Flow Cytometry (F)

Background of BDNF antibody

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kise family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various sigling cascades such the MAPK, PI3K and PLC? pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system. Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both Kv1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the v1.9 + channel in hippocampal neurons. These newly recognized BDNF actions underlie its â??rapidâ? neurol functions that include changes in neurol excitability, plasticity and syptic transmission. Due to the high degree of homology between the different neurotrophins it has been difficult to produce truly specific antibodies against any of them. We have now produced a highly specific antibody against BDNF which recognizes exclusively BDNF.

General readings

Egan M.F., et al (2003). Cell 112:257-269.Ribases M., et al (2003). Mol. Psychiatry 8:745-751.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BDNF antibody

BDNF Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

BDNF antibody

BDNF Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

BDNF antibody

Staining U-2 OS and U-251 MG cells using BDNF Antibody Cat.-No PP1000P Rabbit Aff - Purified Hu, Ms, Rt C, E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

BDNF antibody

Staining U-2 OS and U-251 MG cells using BDNF Antibody Cat.-No PP1000P Rabbit Aff - Purified Hu, Ms, Rt C, E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

BDNF antibody

Rat DRG stained with DAPI. Rabbit IgG Purified Ms, Rt C, ICC/IF, WB
0.5 mg / €400.00
  Novus Biologicals Inc.

BDNF antibody

Anti-human BDNF antibody in WB shows detection of recombinant human BDNF raised in E.coli. Recombinant truncated (0.1 µg, 27 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was incubated with anti-Human BDNF. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF (19-247) antibody

Predicted band size: 28 kDa . Positive control: Mouse skin tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BDNF (153-168) antibody

Predicted cell location: Cytoplasm . Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/30-150  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and thyroid cancer tissue using BDNF antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BDNF antibody

Western blot analysis of extracts of SH-SY5Y cell and PC-12 cell lines, using BDNF antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

This antibody stained U-2 OS and U-251 MG  cells. The primary antibody was  incubated at 2.0 ug/ml overnight at 4C followed by a fluorescent labeled  secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of  the Cell Profiling group, SciLifeLab Stockholm. Rabbit IgG Purified Hu C, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: 1, 2. Anti-proBDNF antibody (1:200). 3, 4. Anti-proBDNF antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt C, WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

BDNF antibody

Western blot analysis with Anti-BDNF antibody : 1, 5. hBDNF. 2, 6. proBDNF (WT-mouse). 3, 7. hß-NGF . 4, 8. hNT-3. Lanes 1-4 Anti-BDNF antibody (1:200). Lanes 5-8 Anti-BDNF antibody, preincubated with the control peptide antigen. Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins. Rabbit Purified Hu, Ms, Rt C, WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

BDNF antibody

TA348982 (5 ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining. Goat Aff - Purified Can, Hu, Ms, Rt C, E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

Western blot analysis of brain tissue lysate using BDNF antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

BDNF antibody

Western Blot analysis of BDNF expression in transfected 293T cell line (H00000627-T01) by BDNF MaxPab polyclonal antibody.

Lane 1: BDNF transfected lysate(27.28 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu, Ms E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

BDNF antibody

Immunofluorescence of purified MaxPab antibody to BDNF on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

BDNF antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BDNF and NTF4 HeLa cells were stained with anti-BDNF rabbit purified polyclonal 1:1200 and anti-NTF4 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BDNF antibody

Western Blot analysis of BDNF expression in transfected 293T cell line by BDNF monoclonal antibody (M02), clone 1B10.

Lane 1: BDNF transfected lysate(28.9 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BDNF antibody

BDNF Rabbit Hu, Rt C, E, WB
0.5 mg / €390.00
  Abnova Taiwan Corp.

BDNF antibody

BDNF Rabbit Hu, Rt C, E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

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