BDNF antibody

Principal name

BDNF antibody

Alternative names for BDNF antibody

Brain-derived neurotrophic factor, Abrineurin

SwissProt ID

P14082 (Pig), P21237 (Mouse), P23363 (Rat), P23560 (Human), P25429 (Chick), Q0EAB7 (Horse), Q95106 (Bovin)

Gene ID

627 (BDNF), 24225 (Bdnf), 12064

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Chicken, Rabbit, Mouse, Sheep, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), ELISA (detection) (E(detection)), Dot blot (Dot), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BDNF antibody

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kise family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various sigling cascades such the MAPK, PI3K and PLC? pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system. Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both Kv1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the v1.9 + channel in hippocampal neurons. These newly recognized BDNF actions underlie its â??rapidâ? neurol functions that include changes in neurol excitability, plasticity and syptic transmission. Due to the high degree of homology between the different neurotrophins it has been difficult to produce truly specific antibodies against any of them. We have now produced a highly specific antibody against BDNF which recognizes exclusively BDNF.

General readings

Egan M.F., et al (2003). Cell 112:257-269.Ribases M., et al (2003). Mol. Psychiatry 8:745-751.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BDNF antibody

BDNF Rabbit IgG Biotin Hu, Ms, Rt E, WB
25 µg / €230.00
  Acris Antibodies GmbH

BDNF antibody

BDNF Rabbit IgG Biotin Hu, Ms, Rt E, WB
50 µg / €340.00
  Acris Antibodies GmbH

BDNF antibody

Staining U-2 OS and U-251 MG cells using BDNF Antibody Cat.-No PP1000P Rabbit IgG Aff - Purified Hu, Ms, Rt C, E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

BDNF antibody

Staining U-2 OS and U-251 MG cells using BDNF Antibody Cat.-No PP1000P Rabbit IgG Aff - Purified Hu, Ms, Rt C, E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

BDNF antibody

  Rabbit polyclonal anti-BDNF antibody  
Anti-human BDNF antibody in WB shows detection of recombinant human BDNF raised in E.coli. Recombinant truncated (0.1 µg, 27 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was incubated with anti-Human BDNF. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Rabbit IgG Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

BDNF (19-247) antibody

  Anti-BDNF Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: Mouse skin tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BDNF (153-168) antibody

  Anti-BDNF Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/30-150  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and thyroid cancer tissue using BDNF antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BDNF antibody

  Rabbit anti-BDNF Polyclonal Antibody  
Western blot analysis of extracts of SH-SY5Y cell and PC-12 cell lines, using BDNF antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

  Anti-Human BDNF Rabbit Polyclonal Antibody  
This antibody stained U-2 OS and U-251 MG  cells. The primary antibody was  incubated at 2.0 ug/ml overnight at 4C followed by a fluorescent labeled  secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of  the Cell Profiling group, SciLifeLab Stockholm. Rabbit IgG Purified Hu C, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

  Rabbit Polyclonal Anti-proBDNF  
Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: 1, 2. Anti-proBDNF antibody (1:200). 3, 4. Anti-proBDNF antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt C, WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

BDNF antibody

  Rabbit Polyclonal Anti-BDNF  
Western blot analysis with Anti-BDNF antibody : 1, 5. hBDNF. 2, 6. proBDNF (WT-mouse). 3, 7. hß-NGF . 4, 8. hNT-3. Lanes 1-4 Anti-BDNF antibody (1:200). Lanes 5-8 Anti-BDNF antibody, preincubated with the control peptide antigen. Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins. Rabbit Purified Hu, Ms, Rt C, WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

BDNF antibody

  Rabbit Polyclonal Anti-BDNF Antibody  
Host: Rabbit; Target Name: BDNF; Sample Tissue: HepG2 Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

  Goat Polyclonal Anti-BDNF Antibody  
TA348982 (5 ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining. Goat Aff - Purified Can, Hu, Ms, Rt C, E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

BDNF antibody

Western blot analysis of brain tissue lysate using BDNF antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

BDNF antibody

BDNF Chicken Aff - Purified Hu, Rt C, E, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

BDNF antibody

Intracellular staining of activated human T cells with APC-conjugated anti-human BDNF-APC, filled histogram) or isotype control (open histogram). Mouse IgG1 35909 APC Hu F
100 Tests / €440.00
  Neuromics Antibodies

BDNF antibody

BDNF (dilution-3.8g/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P
0.1 mg / €420.00
  Neuromics Antibodies

BDNF antibody

BDNF Mouse IgG 35928 Aff - Purified Hu E, P, WB
0.5 mg / €800.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

BDNF - human gene knockout kit via CRISPR

BDNF - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

BDNF - human gene knockout kit via CRISPR

BDNF - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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