Bestrophin-1 antibody

Principal name

Bestrophin-1 antibody

Alternative names for Bestrophin-1 antibody

BEST1, VMD2, TU15B, Vitelliform macular dystrophy protein 2

SwissProt ID

O76090 (Human), O88870 (Mouse), Q8WMR7 (Pig)

Gene ID

7439 (BEST1), 24115 (Best1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Por (Porcine), Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Bestrophin-1 antibody

Mammalian Cl channels can be broadly classified into four different families: voltage-dependent Cl channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl channels (?-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl channels (Bestrophin and Anoctamin channels). Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy (BVMD). BVMD is mainly electrophysiologically characterized by a decrease in the light peak and physiologically by the thinning of the reti layer which eventually leads to the loss of central vision. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the R level. In addition, splice variants of some of these Ca2+-activated Cl channels (CaCCs) have also been detected. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. Recently, Bestrophin-1 was shown to be functiolly expressed in astrocytes in both primary cell culture and in situ. Bestrophin-1 is also detected in reti, brain, spil cord and testes. Two different topologies for Bestrophin-1 have been proposed. The first, the preferred structure, proposes that six hydrophobic domains span the membrane, while the second suggests that there are only four membrane-spanning domains. Bestrophin-1, along with its counterparts, is activated by intracellular Ca2+. A recent study demonstrated that Bestrophin-1 indeed binds Ca2+ and by mutating specific residues, showed which amino acid residues are essential for binding Ca2+, providing additiol evidence that Bestrophin-1 is activated by direct binding of Ca2+ to the channel. Bestrophin-1 has been found to release glutamate from astrocytes and is located at microdomains near sypses.

General readings

Sodi A, Passerini I, et al. (2012). Mol Vis.18:2736-48.
Bitner H, Schatz P, et al. (2012). Am J Ophthalmol. 154(2):403-412.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bestrophin-1 antibody

  Mouse Monoclonal Bestrophin 1 Antibody (E6-6)  
Western Blot: Bestrophin Antibody (E6-6) - Western blot detection of Bestrophin (68 kDa) from human RPE cell lysate. Mouse IgG1 E6-6 Purified Hu, Por, Prim ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bestrophin-1 antibody

  Anti-BEST1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using BEST1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Bestrophin-1 antibody

  Rabbit Polyclonal Anti-Bestrophin-1 (extracellular)  
Western blot analysis of rat lung (lanes 1 and 3) and rat eye (lanes 2 and 4) lysate: 1,2. Anti-Bestrophin-1 (extracellular) antibody, (1:200). 3,4. Anti-Bestrophin-1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Bestrophin-1 antibody

  Rabbit anti-BEST1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using BEST1 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bestrophin-1 antibody

Bestrophin-1 Mouse IgG Ascites Hu, Por, Prim ICC/IF, WB
0.1 ml / €370.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

BEST1 - human gene knockout kit via CRISPR

BEST1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

BEST1 - human gene knockout kit via CRISPR

BEST1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BEST1 - human gene knockout kit via CRISPR

BEST1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BEST1 - human gene knockout kit via CRISPR

BEST1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Best1 - mouse gene knockout kit via CRISPR

Best1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Best1 - mouse gene knockout kit via CRISPR

Best1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Best1 - mouse gene knockout kit via CRISPR

Best1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Best1 - mouse gene knockout kit via CRISPR

Best1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Bestrophin-1 (transcript variant 1)

  Recombinant protein of human bestrophin 1 (BEST1), transcript variant 1  
Bestrophin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Bestrophin-1 (292-585, His-tag)

Bestrophin-1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Bestrophin-1 (292-585, His-tag)

Bestrophin-1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

BEST1 overexpression lysate

BEST1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, BEST1 (mGFP-tagged) - Human bestrophin 1 (BEST1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, BEST1 (mGFP-tagged) - Human bestrophin 1 (BEST1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,230.00
  OriGene Technologies, Inc.

Lenti ORF particles, BEST1 (mGFP-tagged) - Human bestrophin 1 (BEST1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, BEST1 (mGFP-tagged) - Human bestrophin 1 (BEST1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, BEST1 (Myc-DDK tagged) - Human bestrophin 1 (BEST1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, BEST1 (Myc-DDK tagged) - Human bestrophin 1 (BEST1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.

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