Bestrophin-2 antibody

Principal name

Bestrophin-2 antibody

Alternative names for Bestrophin-2 antibody

BEST2, VMD2L1, Vitelliform macular dystrophy 2-like protein 1

SwissProt ID

Q8BGM5 (Mouse), Q8NFU1 (Human)

Gene ID

54831 (BEST2), 212989 (Best2)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Hst (Hamster), Por (Porcine), Rb (Rabbit), Gorilla

Available hosts

Rabbit

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Bestrophin-2 antibody

In the recent past at least 3 different family for “chloride channel” have been characterized: ClC family, ligand gated channels for the GABA and glycine receptor family and the cystic fibrosis membrane conductance regulator. These chloride channels play important role in maintaining resting potentials, ion refluxes, acidification of internal organnles such as lysosomes in both excitatory and non-excitatory nerve and muscle cells. The bestrophins are a newly described family of “anion channels” unrelated in primary sequence to any previously characterized channel proteins. Bestrophins were originally defined as a family of over 20 related sequences of the C. elegans. The first mammalian bestrophin was identified as the vitelliform macular dystrophy (VMD), 1 also known as Best disease (1). Three more members of the bestrophin family members were cloned and identified recently, Bestrophin 2, 3 and 4. The bestrophin family members are membrane protein with 2-TMD and have a conserved 350-400 amino domain including the invariant peptide motif RFP. Each of the Bestrophin proteins has a unique C-terminus that lack similarity to other proteins or motifs. Bestrophin 1 gene is localized to chromosome 19p13.2-p13.12, Bestrophin 2 to 1p32.3-p33 and Bestrophin 3 to 12q14.2-q15. RT-PCR analyses revealed tissue-restricted expression of the three genes with both Bestrophin 1 and Bestrophin 2 are abundantly transcribed in colon. Bestrophin 1 is present in the retinal pigment epithelium while Bestrophin 3 shows predominant expression in skeletal muscle (2, 3). Functionally the Bestrophins oligomerise to form tetramers and pentamers in order to act as calcium sensitive chloride channels. It has been shown that Bestrophin interacts with beta catalytic subunit of protein phosphatase 2A (PP2Ac). Such Protein-protein interaction between bestrophin and PP2Ac and the structural subunit of PP2A, PR65, was confirmed by reciprocal immunoprecipitation. The interaction between PP2Ac and the Bestrophin takes place near the Carboxy-terminal end of the protein. Okadic acid induce the phosphorylation of Bestrophin in vitro. Bestrophin also serves in the signal transduction pathway that modulates the light peak of the EOG, that is regulated by phosphorylation of the Bestrophin that in turn is regulated by protein phosphatase 2A (PP2A).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bestrophin-2 antibody

Anti-BEST2 / Bestrophin-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Bov, Can, Gorilla, Hst, Hu, Mky, Ms, Por, Rb, Rt
50 µg / €345.00
  OriGene Technologies, Inc.

Bestrophin-2 antibody

Anti-BEST2 / Bestrophin-2 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Bov, Gorilla, Hst, Hu, Mky, Ms
50 µg / €345.00
  OriGene Technologies, Inc.

Bestrophin-2 antibody

Western blot analysis of rat colon membranes: 1. Anti-Bestrophin-2 (extracellular) antibody, (1:200). 2. Anti-Bestrophin-2 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Bestrophin-2 control peptide

Bestrophin-2
0.25 mg / €250.00
  Acris Antibodies GmbH

Bestrophin-2 (C-term) antibody

Bestrophin-2 Rabbit Aff - Purified Hu, Mky, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Bestrophin-2 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Bestrophin-2 antibody

Western blot analysis of Bestrophin-2 expression in mouse brain (A); rat brain (B) whole cell lysates. Rabbit Aff - Purified Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Bestrophin-2 antibody

Western blot analysis of Bestrophin-2 expression in mouse brain (A); rat brain (B) whole cell lysates. Rabbit Aff - Purified Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Bestrophin-2 antibody

Western blot analysis of Bestrophin-2 expression in mouse brain (A); rat brain (B) whole cell lysates. Rabbit Aff - Purified Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Bestrophin-2 antibody

Lane 1 HEK293 cells transfected with hBest2 and Lane 2 HEK 293 cells transfected with an hBest2-GFP fusion. Rabbit Aff - Purified Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Bestrophin-2 antibody

Immunohistochemistry-Paraffin: Bestrophin 2 Antibody [NBP1-91699] Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

BEST2 overexpression lysate

BEST2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

12 Item(s)

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