Bestrophin-3 antibody

Principal name

Bestrophin-3 antibody

Alternative names for Bestrophin-3 antibody

BEST3, VMD2L3, Vitelliform macular dystrophy 2-like protein 3

SwissProt ID

Q6H1V1 (Mouse), Q8N1M1 (Human)

Gene ID

144453 (BEST3), 382427 (Best3)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Bt (Bat), Hst (Hamster)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Luminex (LMNX)

Background of Bestrophin-3 antibody

In the recent past at least 3 different family for “chloride channel” have been characterized: ClC family, ligand gated channels for the GABA and glycine receptor family and the cystic fibrosis membrane conductance regulator. These chloride channels play important role in maintingining resting potentials, ion refluxes, acidification of internal organnles such as lysosomes in both excitatory and non-excitatory nerve and muscle cells. The Bestrophins are a newly described family of “anion channels” unrelated in primary sequence to any previously characterized channel proteins. Bestrophins were originally defined as a family of over 20 related sequences of the C. elegans. The first mammalian bestrophin was identified as the vitelliform macular dystrophy (VMD), 1 also known as Best disease (1). Three more members of the bestrophin family members were cloned and identified recently, Bestrophin 2, 3 and 4. The bestrophin family members are membrane protein with 2-TMD and have a conserved 350-400 amino domain including the invariant peptide motif RFP. Each of the Bestrophin proteins has a unique C-terminus that lack similarity to other proteins or motifs. Bestrophin 1 gene is localized to chromosome 19p13.2-p13.12, Bestrophin 2 to 1p32.3-p33 and Bestrophin 3 to 12q14.2-q15. RT-PCR analyses revealed tissue-restricted expression of the three genes with both Bestrophin 1 and Bestrophin 2 are abundantly transcribed in colon. Bestrophin 1 is present in the retinal pigment epithelium while Bestrophin 3 shows predominant expression in skeletal muscle (2, 3). Functionally the bestrophines oligomerize to form tetramers and pentamers in order to act as calcium sensitive chloride channels. It has been shown that Bestrophin interacts with beta catalytic subunit of protein phosphatase 2A (PP2Ac). Such Protein-protein interaction between bestrophin and PP2Ac and the structural subunit of PP2A, PR65, was confirmed by reciprocal immunoprecipitation. The interaction between PP2Ac and the Bestrophin takes place near the Carboxy-terminal end of the protein. Okadic acid induce the phosphorylation of Bestrophin in vitro. Bestrophin also serves in the signal transduction pathway that modulates the light peak of the EOG, that is regulated by phosphorylation of the Bestrophin that in turn is regulated by protein phosphatase 2A (PP2A).

General readings

1. Marmorstein LY, McLaughlin PJ, Stanton JB, Yan L, Crabb JW, Marmorstein AD. J. Biol. Chemistry 2002; June 10, Electronic publication.
2. Stohr H, Marquardt A, et. al., Eur J Hum Genet. 2002 Apr;10(4):281-4.
3. Takashi Tsunenari , Hui Sun, John Williams, Hugh Cahill , Philip Smallwood, King-Wai Yau ** and Jeremy
Nathans. J. Biol. Chem., Vol. 278, Issue 42, 41114-41125, October 17, 2003.

  1. Quick-Filters:
  2. Highly Validated (4)

Items 1 to 20 of 32 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bestrophin-3 antibody

  BEST3 (Bestrophin 3) mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)  
Anti-BEST3 mouse monoclonal antibody (TA501979) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436). Mouse IgG1 OTI2H3 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Bestrophin-3 antibody

  BEST3 (Bestrophin 3) mouse monoclonal antibody, clone OTI3D7 (formerly 3D7)  
Anti-BEST3 mouse monoclonal antibody (TA502154) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436). Mouse IgG2a OTI3D7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Bestrophin-3 antibody

  Luminex validated BEST3 mouse monoclonal antibody (clone 1B6, clone OTI1B6 (formerly 1B6)  
BEST3 ELISA with 1B6 Capture (TA600081) and 2H3 Detection (TA700082) Antibodies. Substrate used: Recombinant Human BEST3 (TP318436) Mouse IgG1 OTI1B6 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bestrophin-3 antibody

  ELISA and Luminex validated biotinylated BEST3 mouse monoclonal antibody (clone 2H3), clone OTI2H3 (formerly 2H3)  
BEST3 ELISA with 1B6 Capture (TA600081) and 2H3 Detection (TA700082) Antibodies. Substrate used: Recombinant Human BEST3 (TP318436) Mouse IgG1 OTI2H3 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Bestrophin-3 antibody

  Rabbit Polyclonal Anti-BEST3 Antibody (C-Terminus)  
Anti-BEST3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

Bestrophin-3 antibody

  Rabbit Polyclonal Anti-BEST3 Antibody (Internal)  
Anti-BEST3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bov, Bt, Hst, Hu, Mky, Por
50 µg / €379.00
  OriGene Technologies, Inc.

Bestrophin-3 antibody

  BEST3 (Bestrophin 3) mouse monoclonal antibody, clone OTI3B11 (formerly 3B11)  
Anti-BEST3 mouse monoclonal antibody (TA501768) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436). Mouse IgG2a OTI3B11 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Bestrophin-3 antibody

  anti-BEST3 (Bestrophin 3) mouse monoclonal antibody, clone OTI1B6 (formerly 1B6)  
Anti-BEST3 mouse monoclonal antibody (TA501826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436). Mouse IgG2b OTI1B6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Bestrophin-3 antibody

  BEST3 (Bestrophin 3) mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)  
Anti-BEST3 mouse monoclonal antibody (TA501878) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436). Mouse IgG1 OTI1H5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Bestrophin-3 antibody

  ELISA and Luminex validated BEST3 mouse monoclonal antibody (clone 3B1, clone OTI3B11 (formerly 3B11)  
BEST3 ELISA with 3B11 Capture (TA600082) and 2H3 Detection (TA700082) Antibodies. Substrate used: Recombinant Human BEST3 (TP318436) Mouse IgG1 OTI3B11 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bestrophin-3 antibody

  Luminex validated biotinylated BEST3 mouse monoclonal antibody (clone 3B11), clone OTI3B11 (formerly 3B11)  
BEST3 Luminex with 1B6 Capture (TA600081) and 3B11 Detection (TA700081) Antibodies. Substrate used: Recombinant Human BEST3 (TP318436) Mouse IgG1 OTI3B11 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Bestrophin-3 antibody

  Luminex validated biotinylated BEST3 mouse monoclonal antibody (clone 3D7), clone OTI3D7 (formerly 3D7)  
BEST3 Luminex with 1B6 Capture (TA600081) and 3D7 Detection (TA700132) Antibodies. Substrate used: Recombinant Human BEST3 (TP318436) Mouse IgG1 OTI3D7 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Bestrophin-3 Control Peptide

Bestrophin-3
0.25 mg / €250.00
  Acris Antibodies GmbH

Bestrophin-3 antibody

Bestrophin-3 Rabbit Aff - Purified Hu, Mky, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Bestrophin-3 antibody

Bestrophin-3 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Bestrophin-3 (C-term) antibody

Bestrophin-3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Bestrophin-3 antibody

Human Muscle; WB Suggested Anti-BEST3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; BEST3 antibody - N-terminal region (ARP50109_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

BEST3 - human gene knockout kit via CRISPR

BEST3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

BEST3 - human gene knockout kit via CRISPR

BEST3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BEST3 - human gene knockout kit via CRISPR

BEST3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Items 1 to 20 of 32 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn