Beta-2-microglobulin antibody

Principal name

Beta-2-microglobulin antibody

Alternative names for Beta-2-microglobulin antibody

B2M, Beta-2 microglobulin, Beta 2 microglobulin

SwissProt ID

P01887 (Mouse), P01888 (Bovin), P07151 (Rat), P21611 (Chick), P61769 (Human), Q07717 (Pig)

Gene ID

567 (B2M), 24223 (B2m), 4456713 (acd), 414830

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Chk (Chicken), Bov (Bovine), Tr (Turkey), Ms (Mouse)

Available hosts

Mouse, Rabbit, Sheep, Rat, Chicken, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Radioimmunoassay (R), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoelectron Microscopy (EM), Dot blot (Dot), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Beta-2-microglobulin antibody

Beta2 microglobulin, also known as B2M, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. B2M is a protein found on the surface of many cells and plentiful on the surface of white blood cells. Increased production or destruction of these cells causes B2M levels in the blood to increase. This increase is seen in people with cancers involving white blood cells, but it is particularly meaningful in people newly diagnosed with multiple myeloma. Multiple myeloma is a malignancy (cancer) of a certain kind of white blood cell, called a plasma cell. B2M Testing is done primarily when evaluating a person for certain kinds of cancer affecting white blood cells including chronic lymphocytic leukemia, non-Hodgkin's lymphoma, and multiple myeloma or kidney disease. Recombinant B2M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Drbal K et al. A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood 98:1561-6 (2001). PubMed: 11520808
Hilgert I et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984). PubMed: 6394369
Search PubMed (MEDLINE) for references to beta 2 Microglobulin

  1. Quick-Filters:
  2. Cited (2)
  3. Highly Validated (5)
  4. Overnight Delivery (1)

Items 1 to 20 of 101 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-2-microglobulin antibody

Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE. Mouse IgG2a B2M-01 Biotin Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

Beta-2-microglobulin antibody

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-Human β2-microglobulin antibody (B2M-01). 
Lane 1: RAJI Human Burkitt lymphoma cell line.
Lane 2: EL4 Mouse lymphoblastic lymphoma cell line. 
Lane 3: U937 Human histiocytic lymphoma cell line. Mouse IgG2a B2M-01 HRP Hu E, WB
0.1 mg / €280.00
  Acris Antibodies GmbH

Beta-2-microglobulin antibody

Western blot analysis of extracts of various cell lines, using B2M antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171) Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802776) Mouse IgG1 OTI1A9 Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Beta-2-microglobulin antibody

1. Murine 4T1 cells were mixed with human DU-145 cells and stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Red stained cells mark the Beta-2-microglobulin positive human DU-145 cells.
2. Murine 4T1 cells were stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Murine 4T1 cells are negative for Beta-2-microglobulin staining.
Pictures by courtesy of Fraunhofer ITEM, Regensburg, Germany. Mouse IgG2a B2M-01 Purified Hu E, F, ICC/IF, IP, P, R, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

Beta-2-microglobulin antibody

Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml] Mouse IgG2b 3F9-2C2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Beta-2-microglobulin (65-79) antibody

B2M antibody Cat.-No AP31992PU-N staining of Human Duodenum lysate at 0.03 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Beta-2-microglobulin (N-term) antibody

(0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Mouse IgG1 2213 Purified Hu E
50 µl / €345.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Anti-B2M antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG2a 6D585 Purified Hu E, F, IP, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

IHC of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Rabbit IgG Purified Hu E, P, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

IHC of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Rabbit Purified Hu C, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Western blot analysis of B2M in SK-N-SH cell lysate with B2M antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Predicted band size: 14 kDa . Positive control: Raji cell and human thyroid cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 15%SDS-PAGE Lane1: Raji cell lysate Lane2: Human thyroid cancer tissue lysate Lysate:s 40 ug per lane Primary antibody: 1/125 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Predicted band size: 14 kDa . Positive control: Human kidney tissue and Raji cell lysate. Recommended dilution: 1/200-1000. (Gel: 15%SDS-PAGE Lane1: Human kidney tissue lysate Lane2: Raji cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803617) Mouse IgG1 OTI1A7 Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Beta-2-microglobulin antibody

Methanol-fixed Hela cells stained with 20 μg/ml mouse anti-B2MG mAb clone D6E4 and fluorescence labelled secondary antibody. Mouse IgG1 D6E4 Aff - Purified Hu E, ICC/IF
0.5 mg / €430.00
  Acris Antibodies GmbH

Beta-2-microglobulin antibody

Staining of methanol-fixed Hela cells by Immunocytochemistry using Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2E9) at 1/50 (20 µg/ml). Mouse IgG1 D2E9 Aff - Purified Hu E, E(capture), F, ICC/IF, WB
0.5 mg / €430.00
  Acris Antibodies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Mouse IgG1 F21-21 Biotin Chk, Tr F
0.5 mg / €410.00
  Acris Antibodies GmbH

Items 1 to 20 of 101 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn