Beta-adducin (ADD2) antibody

Principal name

Beta-adducin (ADD2) antibody

Alternative names for Beta-adducin (ADD2) antibody

ADDB, Adducin 2, Erythrocyte adducin subunit beta

SwissProt ID

P35612 (Human), Q05764 (Rat), Q9QYB8 (Mouse)

Gene ID

119 (ADD2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Beta-adducin (ADD2) antibody

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3\'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.

General readings

Kato,N., (2008) Hum. Mol. Genet. 17 (4), 617-627

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-adducin (ADD2) antibody

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using ADD2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-adducin (ADD2) antibody

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using ADD2 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-adducin (ADD2) antibody

WB Suggested Anti-ADD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Beta-adducin (ADD2) antibody

Western blot analysis of Beta-Adducin Antibody (Cat.-No AP20545PU-N) at 1/500 dilution.Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: Rat spleen tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Beta-adducin (ADD2) antibody

Western Blot analysis of ADD2 expression in transfected 293T cell line (H00000119-T02) by ADD2 MaxPab polyclonal antibody.

Lane 1: ADD2 transfected lysate(79.86 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Beta-adducin (ADD2) antibody

Beta-adducin (ADD2) Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Beta-adducin (ADD2) antibody

Western Blot analysis of ADD2 expression in transfected 293T cell line (H00000119-T02) by ADD2 MaxPab polyclonal antibody.

Lane 1: ADD2 transfected lysate(80.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Beta-adducin (ADD2) antibody

Immunofluorescence analysis of HeLa cells, using ADD2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Beta-adducin (ADD2) antibody

Immunofluorescence analysis of HeLa cells, using ADD2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Beta-adducin (ADD2) antibody

Mouse; mouse HEI-OCI auditory; ADD2 antibody - middle region (ARP52378_P050) in Mouse cells using Immunofluorescence Rabbit Aff - Purified Bov, Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Beta-adducin (ADD2) antibody

Western Blot: beta Adducin Antibody [NBP1-19700] - (WB) analysis of Adducin ß (E497) antibody in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Beta-adducin (ADD2) antibody

Western Blot: beta Adducin Antibody [NBP1-53079] - This Anti-ADD2 antibody was used in Western Blot of Transfected 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Beta-adducin (ADD2) antibody

Beta-adducin (ADD2) Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Beta-adducin (ADD2) antibody

Beta-adducin (ADD2) Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Beta-adducin (ADD2) (transcript variant beta-4)

Beta-adducin (ADD2) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Beta-adducin (ADD2) (transcript variant beta-1)

Beta-adducin (ADD2) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Beta-adducin (ADD2) (transcript variant beta-2)

Beta-adducin (ADD2) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Beta-adducin (ADD2)

Beta-adducin (ADD2) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Beta-adducin (ADD2)

Beta-adducin (ADD2) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

ADD2 (Human) IP-WB Antibody Pair

ADD2 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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