Beta-adducin (ADD2) antibody

Principal name

Beta-adducin (ADD2) antibody

Alternative names for Beta-adducin (ADD2) antibody

ADDB, Adducin 2, Erythrocyte adducin subunit beta

SwissProt ID

P35612 (Human), Q05764 (Rat), Q9QYB8 (Mouse)

Gene ID

119 (ADD2), 11519 (Add2), 24171

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P)

Background of Beta-adducin (ADD2) antibody

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3\'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.

General readings

Kato,N., (2008) Hum. Mol. Genet. 17 (4), 617-627

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-adducin (ADD2) antibody

  Rabbit Polyclonal Anti-ADD2 Antibody  
WB Suggested Anti-ADD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt ICC/IF, WB
50 µg / €360.00
  OriGene Technologies, Inc.

Beta-adducin (ADD2) antibody

Western blot analysis of Beta-Adducin Antibody (Cat.-No AP20545PU-N) at 1/500 dilution.Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: Rat spleen tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  OriGene Technologies GmbH

Beta-adducin (ADD2) antibody

Immunofluorescence analysis of HeLa cells, using ADD2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

Beta-adducin (ADD2) antibody

Immunofluorescence analysis of HeLa cells, using ADD2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

4 Item(s)

per page
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