Beta-ARK-1 / ADRBK1 antibody

Principal name

Beta-ARK-1 / ADRBK1 antibody

Alternative names for Beta-ARK-1 / ADRBK1 antibody

BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1, G-protein coupled receptor kinase 2

SwissProt ID

P21146 (Bovin), P25098 (Human), P26817 (Rat), Q99MK8 (Mouse)

Gene ID

156 (ADRBK1), 315648 (Ppp2r1b)

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Mky (Monkey), Por (Porcine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E)

Background of Beta-ARK-1 / ADRBK1 antibody

The beta-adrenergic receptor kinase 1 (BARK1) was originally isolated as a ser/thr kinase that phosphorylates cardiac beta-adrenergic receptors. It has subsequently been found to phosphorylate many other kinds of G protein coupled receptors (GPCR), as well. It is now recognized as one of a family of G protein coupled receptor kinases that are important in regulating the activity of GPCRs. Phosphorylation of GCPRs by BARK1, and other related kinases, generally occurs only after the receptor has been activated by ligand binding. The phosphorylation results in the uncoupling of the GPCR from G proteins by binding to beta-arrestin. The binding of beta-arrestin may also result in receptor internalization, mediated by clatherin and dynamin and/or the activation of the MAP kinase cascade. BARK1 is found in many cell types and may also have a role in the regulation of the microtubular cytoskeleton. The levels of BARK1 enzymatic activity, protein and mRNA are all elevated in a variety of human heart disease states, and the effect of this may contribute to the reduced effectiveness of the beta-adrenergic system in these disease states.

General readings

Iaccarino G, Barbato E, Cipolletta E, De Amicis V, Margulies KB, Leosco D, Trimarco B, Koch WJ. Elevated myocardial and lymphocyte GRK2 expression and activity in human heart failure. Eur Heart J. 2005 Sep;26(17):1752-8. Epub 2005 Jul 29. PMID: 16055494

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-ARK-1 / ADRBK1 antibody

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining. Mouse IgG1 3F8 Ascites Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Beta-ARK-1 / ADRBK1 antibody

TA303088 (0.3µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

TA309566 (green) versus neurofilament NF-M (red), in rat cortex, showing mostly large pyramidal neurons.  Photo courtesy of Dr. Gerry Shaw, University of Florida. Mouse IgG1 5D5 Purified Bov, Hu, Rt C, WB
0.25 ml / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ADRBK1 antibody at dilution 1/87, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

Western blot analysis of extracts from NIH-3T3 cells, using GRK2 (Ab-86) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

Western blot analysis of extracts from HuvEc cells, using ARBK1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

Western blot analysis of extracts of Jurkat cell lines, using ADRBK1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

WB Suggested Anti-ADRBK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HCT15 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

WB Suggested Anti-ADRBK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: PANC1 cell lysateADRBK1 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

Western blot detection of GRK2 in Ramos and THP-1 cell lysates using GRK2 mouse mAb (1:500 diluted),with super ECL.Predicted band size:80KDa.Observed band size:80KDa.Exposure time:20S. Mouse IgG1 3F8-G6-C11 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-ARK-1 / ADRBK1 antibody

Immunohistochemistry (IHC) analyzes of GRK2 antibody (Cat.-No.: AP06430PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Beta-ARK-1 / ADRBK1 antibody

Immunohistochemistry (IHC) analyzes of GRK 2 antibody (Cat.-No.: AP21131PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Beta-ARK-1 / ADRBK1 antibody

ADRBK1 monoclonal antibody, clone 5D5 ( Cat # MAB2373 ) ( green ) and neurofilament NF-M ( red ) staining, in rat cortex, showing mostly large pyramidal neurons. Photo courtesy of Dr. Gerry Shaw, University of Florida. Mouse IgG1 5D5 Bov, Hu, Rt C, ICC/IF, WB
0.25 ml / €560.00
  Abnova Taiwan Corp.

Beta-ARK-1 / ADRBK1 antibody

ADRBK1 polyclonal antibody ( Cat # PAB6974 ) ( 0.3 µg/ml ) staining of Daudi cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Beta-ARK-1 / ADRBK1 pSer29 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRK2 (Phospho-Ser29) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Beta-ARK-1 / ADRBK1 pSer29 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRK2 (Phospho-Ser29) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

Beta-ARK-1 / ADRBK1 pSer685 antibody

Western blot analysis of extracts from HT29 cells treated with insulin 0.01U/ml 15', using GRK2 (Phospho-Ser685) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Beta-ARK-1 / ADRBK1 pSer685 antibody

Western blot analysis of extracts from HT29 cells treated with insulin 0.01U/ml 15', using GRK2 (Phospho-Ser685) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Beta-ARK-1 / ADRBK1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GRK2 (Ab-29) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

Beta-ARK-1 / ADRBK1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GRK2 (Ab-29) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

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