Beta-crystallin S antibody

Principal name

Beta-crystallin S antibody

Alternative names for Beta-crystallin S antibody

Gamma-crystallin S, Gamma-S-crystallin, CRYGS, GRYG8

SwissProt ID

A2IBY7 (Canfa), A4L9I8 (Rabit), O35486 (Mouse), P06504 (Bovin), P0C5E9 (Rat), P22914 (Human)

Gene ID

1427 (CRYGS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P)

Background of Beta-crystallin S antibody

Crystallins are group of structural water soluble proteins that constitute 90% of the vertebrate lens. There are 4 distinct classes of crystallins, alfa-, beta-, gamma- and delta-crystallins. The mammalian lens does not express delta-crystallins and it confined to birds lens. The human eye lenses at birth contain at least 14 distinct lenses specific proteins called crystallins, 12 of which are full length proteins are 2 are truncated ranging in molecular weight from 20-30 kDa. The relative proportional expression of alfa and beta crystallins in human eye lenses during the 2-300 days is in integral proportion indicating a molar relation ship between these proteins. During early development the ratios of alfaA and beta B2 crystallin change proportionally to the logrithm of age during the first year with alfa A decreasing and beta B2 decreasing in equimolar ratios. Though the functional significance of the reciprocal decrease in alfaA and an increase in betaB2 is not fully known, the beta crystalline may be substituting the alfa crystalline in later part of life (1). Two major determinants of the transparency of the lens are protein-protein interactions and stability of the crystallins, the structural proteins in the lens. betaB2 is the most abundant beta-crystallin in the human lens and is important in formation of the complex interactions of lens crystallins. Certain post transaltional modifications and formation of homo and heterodimers also pay an in important role in the over all transparency of the eye lenses. The thermodynamic and kinetic stabilities of the eye lens proteins, beta-gamma-crystallins are important in the etiology of senile cataract by controlling the chance of proteins unfolding, which can lead to aggregation and loss of transparency. betaB2-Crystallin orthologs are of low stability and comprise two typical beta-gamma-crystallin domains, although, uniquely, the N-terminal domain has a cysteine in one of the conserved folded beta-hairpins. Most of crystalline genes are located on the human chromosome 2 except gamma S crystalline, located on chromosome 3.
Numerous post-translational changes take place in crystalline popylpeptide including deamidation, cleavage, oxidation, during maturation and ageing of the lens. The nucleotide sequence of the cDNA of eye lens beta s-crystallin has been determined and the amino acid sequence has been confirmed by amino acid compositions and partial sequences of the tryptic peptides of this monomeric protein. beta s-Crystallin has a length of 178 residues, corresponding to a mol. wt. of 21 kDa. The crystalline S has a blocked N-terminal serine. Comparison of beta s with the known sequences of other beta- and gamma-crystallins shows beta s to be more closely related to the monomeric gamma-crystallins than to the oligomeric beta-crystallins. The computer assisted molecular modeling of beta S crystallin suggested similarities with the gamma-crystallins which supports its monomeric behavior (3). The water soluble beta crystalline S also are substrate for lens transglutaminase which catalyze the formation of epsilon and gamma glutamyl-lysine isopeptides cross links between polypeptides (3).

General readings

Sun H., et al. (2005) J Med Genet. 42(9):706-10.
Jester JV., et al. (2008) Semin Cell Dev Biol. 19(2):82-93.

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-crystallin S (19-37) antibody

Western blot of AP09926PU-N (1:750). Approx. MW of Beta Crystallin is 21 kDa. Rabbit Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Beta-crystallin S (C-term) antibody

Beta-crystallin S Rabbit Aff - Purified Hu, Ms, Rt C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Beta-crystallin S (C-term) antibody

CRYGS Antibody (C-term) (Cat. #AP51093PU-N) western blot analysis in mouse spleen tissue lysates (35µg/lane).This demonstrates the CRYGS antibody detected the CRYGS protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-crystallin S antibody

Western Blot analysis of CRYGS expression in transfected 293T cell line (H00001427-T01) by CRYGS MaxPab polyclonal antibody.

Lane 1: CRYGS transfected lysate(19.58 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Beta-crystallin S antibody

Western Blot analysis of CRYGS expression in transfected 293T cell line (H00001427-T02) by CRYGS MaxPab polyclonal antibody.

Lane 1: CRYGS transfected lysate(19.58 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Beta-crystallin S antibody

Immunohistochemistry-Paraffin: CRYGS Antibody [NBP1-84375] - Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in parietal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Beta-crystallin S

Beta-crystallin S Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Beta-crystallin S (1-178, His-tag)

Beta-crystallin S Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

Beta-crystallin S (1-178, His-tag)

Beta-crystallin S Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Beta-crystallin S

Beta-crystallin S Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Beta-crystallin S

Beta-crystallin S Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Beta-crystallin S

Beta-crystallin S Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Beta-crystallin S

Beta-crystallin S Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

CRYGS 293T Cell Transient Overexpression Lysate(Denatured)

CRYGS 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

CRYGS overexpression lysate

CRYGS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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