Beta-crystallin S antibody

Principal name

Beta-crystallin S antibody

Alternative names for Beta-crystallin S antibody

Gamma-crystallin S, Gamma-S-crystallin, CRYGS, GRYG8

SwissProt ID

A2IBY7 (Canfa), A4L9I8 (Rabit), O35486 (Mouse), P06504 (Bovin), P0C5E9 (Rat), P22914 (Human)

Gene ID

1427 (CRYGS), 12970 (Crygs), 689897

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C)

Background of Beta-crystallin S antibody

Crystallins are group of structural water soluble proteins that constitute 90% of the vertebrate lens. There are 4 distinct classes of crystallins, alfa-, beta-, gamma- and delta-crystallins. The mammalian lens does not express delta-crystallins and it confined to birds lens. The human eye lenses at birth contain at least 14 distinct lenses specific proteins called crystallins, 12 of which are full length proteins are 2 are truncated ranging in molecular weight from 20-30 kDa. The relative proportional expression of alfa and beta crystallins in human eye lenses during the 2-300 days is in integral proportion indicating a molar relation ship between these proteins. During early development the ratios of alfaA and beta B2 crystallin change proportionally to the logrithm of age during the first year with alfa A decreasing and beta B2 decreasing in equimolar ratios. Though the functional significance of the reciprocal decrease in alfaA and an increase in betaB2 is not fully known, the beta crystalline may be substituting the alfa crystalline in later part of life (1). Two major determinants of the transparency of the lens are protein-protein interactions and stability of the crystallins, the structural proteins in the lens. betaB2 is the most abundant beta-crystallin in the human lens and is important in formation of the complex interactions of lens crystallins. Certain post transaltional modifications and formation of homo and heterodimers also pay an in important role in the over all transparency of the eye lenses. The thermodynamic and kinetic stabilities of the eye lens proteins, beta-gamma-crystallins are important in the etiology of senile cataract by controlling the chance of proteins unfolding, which can lead to aggregation and loss of transparency. betaB2-Crystallin orthologs are of low stability and comprise two typical beta-gamma-crystallin domains, although, uniquely, the N-terminal domain has a cysteine in one of the conserved folded beta-hairpins. Most of crystalline genes are located on the human chromosome 2 except gamma S crystalline, located on chromosome 3.
Numerous post-translational changes take place in crystalline popylpeptide including deamidation, cleavage, oxidation, during maturation and ageing of the lens. The nucleotide sequence of the cDNA of eye lens beta s-crystallin has been determined and the amino acid sequence has been confirmed by amino acid compositions and partial sequences of the tryptic peptides of this monomeric protein. beta s-Crystallin has a length of 178 residues, corresponding to a mol. wt. of 21 kDa. The crystalline S has a blocked N-terminal serine. Comparison of beta s with the known sequences of other beta- and gamma-crystallins shows beta s to be more closely related to the monomeric gamma-crystallins than to the oligomeric beta-crystallins. The computer assisted molecular modeling of beta S crystallin suggested similarities with the gamma-crystallins which supports its monomeric behavior (3). The water soluble beta crystalline S also are substrate for lens transglutaminase which catalyze the formation of epsilon and gamma glutamyl-lysine isopeptides cross links between polypeptides (3).

General readings

Sun H., et al. (2005) J Med Genet. 42(9):706-10.
Jester JV., et al. (2008) Semin Cell Dev Biol. 19(2):82-93.

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-crystallin S (C-term) antibody

CRYGS Antibody (C-term) (Cat. #AP51093PU-N) western blot analysis in mouse spleen tissue lysates (35µg/lane).This demonstrates the CRYGS antibody detected the CRYGS protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CRYGS - human gene knockout kit via CRISPR

CRYGS - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CRYGS - human gene knockout kit via CRISPR

CRYGS - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CRYGS - human gene knockout kit via CRISPR

CRYGS - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CRYGS - human gene knockout kit via CRISPR

CRYGS - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Beta-crystallin S

  Recombinant protein of human crystallin, gamma S (CRYGS)  
Beta-crystallin S Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Beta-crystallin S (1-178, His-tag)

Beta-crystallin S Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

Beta-crystallin S (1-178, His-tag)

Beta-crystallin S Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

CRYGS overexpression lysate

CRYGS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CRYGS (mGFP-tagged) - Human crystallin, gamma S (CRYGS), 200 uL, >10^7 TU/mL

Lenti ORF particles, CRYGS (mGFP-tagged) - Human crystallin, gamma S (CRYGS), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, CRYGS (Myc-DDK tagged) - Human crystallin, gamma S (CRYGS) , 200 uL, >10^7 TU/mL

Lenti ORF particles, CRYGS (Myc-DDK tagged) - Human crystallin, gamma S (CRYGS) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Crygs (GFP-tagged) - Mouse crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL

Lenti ORF particles, Crygs (GFP-tagged) - Mouse crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Crygs (Myc-DDK-tagged) - Mouse crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL

Lenti ORF particles, Crygs (Myc-DDK-tagged) - Mouse crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Crygs (GFP-tagged ORF) - Rat crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL

Lenti ORF particles, Crygs (GFP-tagged ORF) - Rat crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Crygs (Myc-DDK-tagged ORF) - Rat crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL

Lenti ORF particles, Crygs (Myc-DDK-tagged ORF) - Rat crystallin, gamma S (Crygs), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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