Beta-galactosidase antibody

Principal name

Beta-galactosidase antibody

Alternative names for Beta-galactosidase antibody

Lactase, GLB1, ELNR1, Elastin receptor 1, LacZ

SwissProt ID

O19015 (Felca), P16278 (Human), P23780 (Mouse), Q58D55 (Bovin), Q8FKG6 (Ecol6), Q9TRY9 (Canfa)

Gene ID

2720 (GLB1), 1036228 (lacZ)

Available reactivities

Hu (Human), E. coli (Escherichia coli), Ms (Mouse), Bac (Bacteria), Vertebrates, Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), EPair for Elisa (EPair), Paraffin Sections (P), Flow Cytometry (F)

Background of Beta-galactosidase antibody

The human b-galactosidase gene, known as the LacZ gene, maps to chromosome 3p21.33 and encodes a 677 amino acid protein with an optimum functional pH range of 6 to 8. Catalytically active b-galactosidaseis (b-Gal) is a tetramer of four identical subunits, each with an active site, which can independently catalyze the cleavage of terminal galactose. Monovalent cations have a stimulatory effect on the enzymatic reaction, which likely involves a galactosyl-enzyme complex intermediate. b-Gals are widespread in animals, microorganisms and plants. The LacZ gene is widely used as a reporter gene with a variety of colored or fluorescent compounds capable of being produced from appropriate substrates, such as Xgal, which produces a blue color. For this reason, LacZ is incorporated into numerous plasmid vectors as a marker.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GLB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505568) Mouse IgG2a OTI2F6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Beta-galactosidase antibody

Immunofluorescent staining of HeLa cells using anti-GLB1 mouse monoclonal antibody (TA505625). Mouse IgG1 OTI10B2 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Beta-galactosidase antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GLB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505544) Mouse IgG2a OTI1C9 Purified Can, Hu, Mky P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Beta-galactosidase antibody

Anti-GLB1 mouse monoclonal antibody (TA505597) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GLB1(RC200721). Mouse IgG1 OTI5H2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Beta-galactosidase antibody

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308238 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-galactosidase antibody

Western blot analysis of extracts from 3T3 cells, using GLB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Beta-galactosidase antibody

Western blot analysis of extracts of various cell lines, using GLB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-galactosidase antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GLB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505442) Mouse IgG2a OTI1E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Beta-galactosidase Control Peptide

Beta-galactosidase
50 µg / €160.00
  Acris Antibodies GmbH

Beta-galactosidase antibody

Western blot analysis of (A) 5 and (B) 25 ng of beta-galactosidase with AP30140PU-N beta-galactosidase antibody at 1 μg/ml. Chicken IgG Aff - Purified Bac E, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Beta-galactosidase antibody

GLB1 MaxPab polyclonal antibody. Western Blot analysis of GLB1 expression in human pancreas. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Beta-galactosidase antibody

Beta-galactosidase Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Beta-galactosidase antibody

Beta-galactosidase Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Beta-galactosidase antibody

Western Blot analysis of GLB1 expression in transfected 293T cell line (H00002720-T01) by GLB1 MaxPab polyclonal antibody.

Lane 1: GLB1 transfected lysate(76.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Beta-galactosidase antibody

GLB1 monoclonal antibody (M01), clone 6E7 Western Blot analysis of GLB1 expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 6E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Beta-galactosidase antibody

Western blot using Beta-Galactosidase polyclonal antibody ( Cat # PAB9921 ) shows detection of a band at ~117 kDa ( lane 1 ) corresponding to Beta-Galactosidase in a partially purified preparation ( arrowhead ).
Approximately 1 µg of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,000.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature ( 800 nm channel, green ).
Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit IgG E. coli, Hu, Ms C, E, ICC/IF, IP, WB
1 mg / €390.00
  Abnova Taiwan Corp.

Beta-galactosidase antibody

Western blot analysis of Beta-galactosidase expression in HEK293T (A); HepG2 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Beta-galactosidase antibody

Western blot analysis of Beta-galactosidase expression in HEK293T (A); HepG2 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Beta-galactosidase antibody

Western blot analysis of Beta-galactosidase expression in HEK293T (A); HepG2 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Beta-galactosidase antibody

Western Blot:  GLB1 Antibody [NBP1-32018]- (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE  diluted at 1:1000 Rabbit IgG Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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