Beta-galactosidase tag antibody

Principal name

Beta-galactosidase tag antibody

Alternative names for Beta-galactosidase tag antibody

Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase

SwissProt ID

P00722 (Ecoli)

Gene ID

945006 (lacZ)

Available reactivities

E. coli (Escherichia coli), Aspergillus, Bac (Bacteria), All Species

Available hosts

Mouse, Rabbit, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of Beta-galactosidase tag antibody

Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of ß-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti Beta-galactosidase antibody is ideal for investigators involved in enzyme research.

General readings

This product has been used in:Peterson KR et al. Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39:1-9 (2004). PubMed PMID: 15124222
General / background references:The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970). PubMed PMID: 5310665

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase tag antibody

Western blot of rabbit anti-Beta-galactosidase (E. coli) antibody biotin conjugated. Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated Cat.-No. R1064B) at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer ( for fluorescent Western blotting) for 30 min at RT. Predicted/observed size: 116 kDa, 116 kDa for Beta-galactosidase. Rabbit Biotin E. coli C, E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Western blotting using  fluorescein conjugated anti-Beta-galactosidase  tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase Cat.-No. R1064F diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results. Rabbit FITC E. coli ICC/IF, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Western blotting using Beta-galactosidase antibody Cat.-No R1064PS. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for N<em>on-Reducing Conditions</em> of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under <em>Reducing Conditions</em> are shown on the right. Blots were blocked overnight at 4°C with blocking buffer for fluorescent Western blotting (p/n MB-070). The membrane was probed with Beta-galactosidase antibody Cat.-No R1064P diluted to 1/10,000. Reaction occurred overnight at 4°C. Dylight649™ conjugated goat-anti-rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center). Fluorescence image was captured using the VersaDoc® imaging system developed by BIO-RAD. Other detection systems will yield similar results. Rabbit Purified E. coli C, E, ICC/IF, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag (Center) antibody

Western blot of purified E. coli  beta-galactosidase sample; reduced sample, immunostained by mAb BG-02 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 BG-02 Purified E. coli ICC/IF, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Immunohistochemical detection of beta-galactosidase on paraffin embedded section of pCDNA3-LacZ/293T cells with AM26630AF-N. Mouse IgG1 5A3 Azide Free E. coli ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG Azide Free E. coli Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG Biotin E. coli Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG Aff - Purified E. coli Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Chicken Ig Purified C, ICC/IF, WB
0.4 ml / €290.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Figure 1. Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were  resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to Nitrocellulose and blocking using blocking buffer for fluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase (Cat.-No. R1064F) diluted to 1/10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System. Other detection systems will yield similar results. Rabbit Serum E. coli C, E, P, WB
2 ml / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit HRP E. coli C, E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 BG-02 E. coli ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit C, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 2E9 Purified Aspergillus, E. coli E
1 mg / €310.00
  HyTest Ltd.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 DC1 4C7 Bac E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 DC3 10D6 Purified All Species E
0.1 mg / €340.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 2E9 Bac E
0.2 mg / €250.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Gal present in a partially purified preparation (arrowhead). Approximately 1µg of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye 800 fluorescence image was captured using the Infrared Imaging System. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG FITC Bac E, ICC/IF, WB
1 mg / €290.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Rabbit IgG HRP Bac E, P, WB
1 mg / €310.00
  Novus Biologicals Inc.

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 BG-02 Aff - Purified Bac ICC/IF, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

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