Beta-galactosidase tag antibody

Principal name

Beta-galactosidase tag antibody

Alternative names for Beta-galactosidase tag antibody

Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase

SwissProt ID

P00722 (Ecoli)

Gene ID

945006 (lacZ)

Available reactivities

E. coli (Escherichia coli), Aspergillus, Bac (Bacteria), All Species

Available hosts

Mouse, Rabbit, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of Beta-galactosidase tag antibody

Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of ß-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti Beta-galactosidase antibody is ideal for investigators involved in enzyme research.

General readings

This product has been used in:Peterson KR et al. Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39:1-9 (2004). PubMed PMID: 15124222
General / background references:The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970). PubMed PMID: 5310665

  1. Quick-Filters:
  2. Cited (1)

1 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase tag antibody

Western blotting using  fluorescein conjugated anti-Beta-galactosidase  tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase Cat.-No. R1064F diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results. Rabbit FITC E. coli ICC/IF, WB
0.1 mg / €460.00
  OriGene Technologies GmbH

1 Item(s)

per page
  • LinkedIn