Beta-galactosidase tag antibody

Principal name

Beta-galactosidase tag antibody

Alternative names for Beta-galactosidase tag antibody

Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase

SwissProt ID

P00722 (Ecoli)

Gene ID

945006 (lacZ)

Available reactivities

E. coli (Escherichia coli), Aspergillus, Bac (Bacteria), All Species

Available hosts

Mouse, Rabbit, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of Beta-galactosidase tag antibody

Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of ß-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti Beta-galactosidase antibody is ideal for investigators involved in enzyme research.

General readings

This product has been used in:Peterson KR et al. Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39:1-9 (2004). PubMed PMID: 15124222
General / background references:The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970). PubMed PMID: 5310665

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-galactosidase tag antibody

Western blotting using Beta-galactosidase antibody Cat.-No R1064PS. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for N<em>on-Reducing Conditions</em> of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under <em>Reducing Conditions</em> are shown on the right. Blots were blocked overnight at 4°C with blocking buffer for fluorescent Western blotting (p/n MB-070). The membrane was probed with Beta-galactosidase antibody Cat.-No R1064P diluted to 1/10,000. Reaction occurred overnight at 4°C. Dylight649™ conjugated goat-anti-rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center). Fluorescence image was captured using the VersaDoc® imaging system developed by BIO-RAD. Other detection systems will yield similar results. Rabbit Purified E. coli C, E, ICC/IF, IP, P, WB
0.1 mg / €460.00
  OriGene Technologies GmbH

Beta-galactosidase tag (Center) antibody

Western blot of purified E. coli  beta-galactosidase sample; reduced sample, immunostained by mAb BG-02 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 BG-02 Purified E. coli ICC/IF, WB
0.1 mg / €230.00
  OriGene Technologies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Chicken Ig Purified C, ICC/IF, WB
0.4 ml / €340.00
  OriGene Technologies GmbH

Beta-galactosidase tag antibody

Beta-galactosidase tag Mouse IgG1 2E9 Purified Aspergillus, E. coli E
1 mg / €310.00
  HyTest Ltd.

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