Beta-hexosaminidase alpha / HEXA antibody

Principal name

Beta-hexosaminidase alpha / HEXA antibody

Alternative names for Beta-hexosaminidase alpha / HEXA antibody

N-acetyl-beta-glucosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha

SwissProt ID

P06865 (Human), P29416 (Mouse), Q0V8R6 (Bovin), Q641X3 (Rat)

Gene ID

3073 (HEXA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of Beta-hexosaminidase alpha / HEXA antibody

HEXA(hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A , a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able tohydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

General readings

1. Akli, S., Chomel, J.-C., Lacorte, J.-M., Bachner, L., Poenaru, A., Poenaru, L. Ten novel mutations in
the HEXA gene in non-Jewish Tay-Sachs patients. Hum. Molec. Genet. 2: 61-67, 1993.
2. Beutler, E., Kuhl, W., Comings, D. Hexosaminidase isozyme in type O Gm2 gangliosidosis
(Sandhoff-Jatzkewitz disease). Am. J. Hum. Genet. 27: 628-638, 1975.
3. Chern, C. J., Beutler, E., Kuhl, W., Gilbert, F., Mellman, W. J., Croce, C. M. Characterization of
heteropolymeric hexosaminidase A in human x mouse hybrid cells. Proc. Nat. Acad. Sci. 73:
3637-3640, 1976.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-hexosaminidase alpha / HEXA antibody

Western blot analysis using HEXA antibody Cat.-No AM06756PU-N against human HEXA (AA: 29-181) recombinant protein (Expected MW is 43.1 kDa). Mouse IgG2b 3F10 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA antibody

Western blot analysis of extracts of various cell lines, using HEXA antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Beta-hexosaminidase alpha / HEXA antibody

WB Suggested Anti-HEXA Antibody Titration: 1 ug/ml; Positive Control: Fetal kidney lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Beta-hexosaminidase alpha / HEXA (89-529) antibody

Flow cytometry analysis of HEXA in A 549 cell line, staining at 2 - 5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Mouse IgG2a AT20F1 Purified Hu E, F, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA (89-529) antibody

Flow cytometry analysis of HEXA in A 549 cell line, staining at 2 - 5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Mouse IgG2a AT20F1 Purified Hu E, F, WB
50 µl / €220.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA (N-term) antibody

(LEFT) Western blot analysis of HEXA Antibody (N-term) (AP17459PU-N) in HepG2 cell line lysates (35 µg/lane). HEXA (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of HEXA Antibody (N-term) (AP17459PU-N) in mouse liver tissue lysates (35 µg/lane). HEXA (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA antibody

Western blot analysis of HEXA Antibody (Center) (AP18045PU-N) in HepG2 cell line lysates (35ug/lane). HEXA (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA antibody

Western Blot analysis of HEXA expression in transfected 293T cell line (H00003073-T01) by HEXA MaxPab polyclonal antibody.

Lane1:HEXA transfected lysate(58.19 KDa).
Lane2:Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Beta-hexosaminidase alpha / HEXA antibody

Beta-hexosaminidase alpha / HEXA Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Beta-hexosaminidase alpha / HEXA antibody

Western Blot analysis of HEXA expression in transfected 293T cell line (H00003073-T01) by HEXA MaxPab polyclonal antibody.

Lane 1: HEXA transfected lysate(60.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Beta-hexosaminidase alpha / HEXA antibody

Western Blot analysis of HEXA expression in transfected 293T cell line by HEXA monoclonal antibody (M06), clone 3D1.

Lane 1: HEXA transfected lysate(60.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 3D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Beta-hexosaminidase alpha / HEXA antibody

Beta-hexosaminidase alpha / HEXA Mouse IgG1 3D1 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Beta-hexosaminidase alpha / HEXA (C-term) antibody

Human brain; Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5ug/ml using anti-HEXA antibody (ARP61100_P050); HEXA antibody - C-terminal region (ARP61100_P050) in Human brain cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Beta-hexosaminidase alpha / HEXA antibody

Beta-hexosaminidase alpha / HEXA Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Beta-hexosaminidase alpha / HEXA antibody

Beta-hexosaminidase alpha / HEXA Rabbit Aff - Purified Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Beta-hexosaminidase alpha / HEXA

Beta-hexosaminidase alpha / HEXA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Beta-hexosaminidase alpha / HEXA (89-529, His-tag)

Beta-hexosaminidase alpha / HEXA Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA (89-529, His-tag)

Beta-hexosaminidase alpha / HEXA Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA (23-529, His-tag)

Beta-hexosaminidase alpha / HEXA Human Purified > 90 % by SDS - PAGE.
0.25 mg / €940.00
  Acris Antibodies GmbH

Beta-hexosaminidase alpha / HEXA (23-529, His-tag)

Beta-hexosaminidase alpha / HEXA Human Purified > 90 % by SDS - PAGE.
50 µg / €300.00
  Acris Antibodies GmbH

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