Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C), Dot blot (Dot), Radioimmunoassay (R)
Background of Beta-Synuclein / SCNB antibody
The beta-synuclein protein is highly homologous to the alpha-synuclein protein for which two mutations were reported in some familial cases of Parkinson disease. It has been shown that both alpha- and beta-synucleins may be able to inhibit phospholipase D2 selectively (1). Recent studies have shown that the neurodegenerative process in disorders with Lewy body formation, such as Parkinson's disease and dementia with Lewy bodies, is associated with alpha-synuclein accumulation and that beta-synuclein might protect the central nervous system from the neurotoxic effects of alpha-synuclein (2). results raise the possibility that beta-synuclein might be a natural negative regulator of alpha-synuclein aggregation and that a similar class of endogenous factors might regulate the aggregation state of other molecules involved in neurodegeneration. Such an anti-amyloidogenic property of beta-synuclein might also provide a novel strategy for the treatment of neurodegenerative disorders (3).
Immunohistochemical of paraffin-embedded human breast cancer using 10498-1-AP(SNCB antibody) at dilution of 1:10 (under 10x lens)
1X10^6 HepG2 cells were stained with 0.2ug SNCB antibody (10498-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemistry-Paraffin: beta Synuclein Antibody [NBP1-90342] - staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in the molecular layer and cells in the granular layer.
Western Blot: beta Synuclein Antibody [NBP1-90342] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Formalin-fixed and paraffin embedded rat brain labeled with Anti Beta-synuclein/SNCB Polyclonal Antibody, Unconjugated (bs-6939R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Immunofluorescent analysis of Beta-synuclein staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).