Betacellulin antibody

Principal name

Betacellulin antibody

Alternative names for Betacellulin antibody

Probetacellulin, BTC

SwissProt ID

P35070 (Human), Q05928 (Mouse), Q9TTC5 (Bovin)

Gene ID

685 (BTC), 12223 (Btc), 852685 (CDC55)

Available reactivities

Hu (Human), Bov (Bovine), Por (Porcine), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Betacellulin antibody

Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG). These growth factors bind to the EGF receptor and share at least 28 % sequence identity and 100% conservation of the 6 cysteine residue positions in the mature peptide sequence. Recombinant human betacellulin cDNA encodes an 80 amino acid residue polypeptide 7 with a predicted molecular mass of approximately 9.5 kDa. Betacellulin (BTC) was originally isolated as a 32 kDa glycoprotein from the conditioned media of mouse pancreatic beta tumor cells. It is capable of stimulating the proliferation of 3T3 cells, retinal pigment epithelial cells, and smooth muscle cells. The cDNA that encodes human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7). Betacellulin is synthesized as a transmembrane precursor protein and then cleaved to release three types of soluble BTC forms (full length plus two amino-terminal truncation sites), all of which are equally bioactive. Human and mouse precursor forms of BTC exhibit 79 % amino acid sequence identity. Betacellulin is a unique EGF family member in that it binds with high affinity to EGF receptor, ErbB-1, and ErbB-4. Overexpression of betacellulin with ErbB-3 and ErbB-4 are linked to human endometrial adenocarcinoma. 2 Betacellulin has a role in the growth and differentiation of islet cells in the human pancreas.5 Betacellulin is produced in a wide variety of tissues, cultured cells, and various body fluids including milk. It is highly expressed in pancreas as well as liver, kidney, and small intestine. Betacellulin can also be found in lower abundance in testis, ovary, lung, and colon. BTC mRNA has not been found in brain, placenta, spleen, thymus, or peripheral blood leukocytes.

General readings

Gratao AA, et al. (2008) Biol Reprod.78(1):43-52.Genetos DC, et al. (2010) Cell Tissue Res. 340(1):81-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Betacellulin antibody

Western blot analysis of extracts of various cell lines, using BTC antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Betacellulin antibody

Western blot analysis of extracts from 293/Jurkat/HeLa cells, using BTC antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Betacellulin (N-term) antibody

BTC Antibody (N-term) (Cat. #TA324423) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the BTC antibody detected the BTC protein (arrow). Rabbit Ig Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Betacellulin antibody

To detect hBetacellulin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBetacellulin. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Betacellulin antibody

To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Betacellulin (TA328269) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Betacellulin antibody

Betacellulin Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Betacellulin antibody

Betacellulin Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Betacellulin antibody

Betacellulin Rabbit Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Betacellulin antibody

Betacellulin Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Betacellulin antibody

Western Blot analysis of BTC expression in transfected 293T cell line (H00000685-T02) by BTC MaxPab polyclonal antibody.

Lane 1: BTC transfected lysate(19.58 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Betacellulin antibody

Western Blot analysis of BTC expression in transfected 293T cell line (H00000685-T01) by BTC MaxPab polyclonal antibody.

Lane 1: BTC transfected lysate(19.58 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Betacellulin antibody

Western Blot analysis of BTC expression in transfected 293T cell line (H00000685-T01) by BTC MaxPab polyclonal antibody.

Lane 1: BTC transfected lysate(19.58 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Betacellulin antibody

Immunoprecipitation of BTC transfected lysate using anti-BTC MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BTC MaxPab mouse polyclonal antibody (B02) (H00000685-B02). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Betacellulin antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BTC and ERBB2. HeLa cells were stained with anti-BTC rabbit purified polyclonal 1:1200 and anti-ERBB2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Betacellulin antibody

Western blot analysis of Probetacellulin expression in A431 (A); PC3 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Por IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Betacellulin antibody

Western blot analysis of Probetacellulin expression in A431 (A); PC3 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Por IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Betacellulin antibody

Western blot analysis of Probetacellulin expression in A431 (A); PC3 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Por IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Betacellulin

Betacellulin Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 20 µg / €199.00

Special Price: 20 µg / €139.00

  OriGene Technologies, Inc.

Betacellulin

Betacellulin Mouse > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 20 µg / €199.00

Special Price: 20 µg / €139.00

  OriGene Technologies, Inc.

Betacellulin

Betacellulin Human Purified > 98 % > 98 % by SDS-PAGE gel and HPLC analyses E. coli
20 µg / €230.00
  Acris Antibodies GmbH

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