BID antibody

Principal name

BID antibody

Alternative names for BID antibody

p22 BID, BH3-interacting domain death agonist

SwissProt ID

P55957 (Human), P70444 (Mouse), Q4JHS0 (Pig), Q8JGM8 (Chick), Q9JLT6 (Rat)

Gene ID

637 (BID), 12122 (Bid), 64625

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of BID antibody

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.

General readings

Lund T, Stokke T, Olsen OE, Fodstad O.
Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis.
Br J Cancer. 2005 Apr 12; [Epub ahead of print]
PMID: 15827557

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BID antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b UMAB139 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

BID antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b UMAB139 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

BID antibody

Western blot analysis of extracts of HL60 cell lines, using BID antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506756) Mouse IgG2a OTIG10 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506762) Mouse IgG1 OTI4A1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506766) Mouse IgG2a OTI3E9 Purified Hu, Mky, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506767) Mouse IgG2a OTI3A10 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506768) Mouse IgG2b OTI3C8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and tonsil tissues (right) using BID mouse mAb with DAB staining. Mouse IgG1 3C5 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

BID (1-195) antibody

Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Mouse IgG1 4D3 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

BID (1-195) antibody

Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Mouse IgG1 4D3 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

BID antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with BID polyclonal antibody ( Cat # PAB2442 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

BID (C-term) antibody

TA302648 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
20 µg / €200.00
  Acris Antibodies GmbH

BID (C-term) antibody

TA302648 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID (C-term) antibody

Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID (Center) antibody

Western blot analysis of Bid in A549 cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID antibody

Predicted band size: 22kDa. Positive control: Human fetal brain and liver tissue, HT-29 and HepG2 cell lysate. Recommended dilution: 1/200-2000. (Gel: 12%SDS-PAGE Lane1: Human fetal brain tissue lysate Lane2: Human fetal liver tissue lysate Lane3: HT-29 cell lysate Lane4: HepG2 cell lysate Lysates: 30 ug per lane Primary antibody: 1/260 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BID antibody

Predicted band size: 22kDa. Positive control: Human fetal brain tissue, HT-29 and HepG2 cell lysate. Recommended dilution: 1/200-2000. (Gel: 12%SDS-PAGE Lane1: Human fetal brain tissue lysate Lane2: HT-29 cell lysate Lane3: HepG2 cell lysate Lysates: 30 ug per lane Primary antibody: 1/220 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BID antibody

Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-BID antibody (clone 1H11). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection. Rat IgG1 1H11 Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID. Mouse IgG2a OTI2B6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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