BID antibody

Principal name

BID antibody

Alternative names for BID antibody

p22 BID, BH3-interacting domain death agonist

SwissProt ID

P55957 (Human), P70444 (Mouse), Q4JHS0 (Pig), Q8JGM8 (Chick), Q9JLT6 (Rat)

Gene ID

637 (BID), 12122 (Bid), 64625

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)

Background of BID antibody

Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF-alpha apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c. (Ref.1-10)

General readings

Lund T, Stokke T, Olsen OE, Fodstad O. Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein Bim(EL) and induces apoptosis. Br J Cancer. 2005 Apr 12

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BID antibody

  BID mouse monoclonal antibody,clone UMAB139  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b UMAB139 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

BID antibody

  BID mouse monoclonal antibody,clone UMAB139  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b UMAB139 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

BID antibody

  BID Rabbit Polyclonal Antibody  
Western blot analysis of extracts of HL60 cell lines, using BID antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID antibody

  BID mouse monoclonal antibody, clone OTIG10 (formerly G10)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506756) Mouse IgG2a OTIG10 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

  BID mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506762) Mouse IgG1 OTI4A1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

  BID mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506766) Mouse IgG2a OTI3E9 Purified Hu, Mky, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

  BID mouse monoclonal antibody, clone OTI3A10 (formerly 3A10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506767) Mouse IgG2a OTI3A10 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

  BID mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506768) Mouse IgG2b OTI3C8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and tonsil tissues (right) using BID mouse mAb with DAB staining. Mouse IgG1 3C5 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

BID (1-195) antibody

Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Mouse IgG1 4D3 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

BID (1-195) antibody

Western blot analysis: The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Mouse IgG1 4D3 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

BID (C-term) antibody

  Goat Polyclonal Antibody against BID  
TA302648 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID (C-term) antibody

  Rabbit Polyclonal Bid Antibody  
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID (Center) antibody

  Rabbit Polyclonal Bid Antibody  
Western blot analysis of Bid in A549 cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID antibody

  Anti-BID Rabbit Polyclonal Antibody  
Predicted band size: 22kDa. Positive control: Human fetal brain and liver tissue, HT-29 and HepG2 cell lysate. Recommended dilution: 1/200-2000. (Gel: 12%SDS-PAGE Lane1: Human fetal brain tissue lysate Lane2: Human fetal liver tissue lysate Lane3: HT-29 cell lysate Lane4: HepG2 cell lysate Lysates: 30 ug per lane Primary antibody: 1/260 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BID antibody

  Anti-BID Rabbit Polyclonal Antibody  
Predicted band size: 22kDa. Positive control: Human fetal brain tissue, HT-29 and HepG2 cell lysate. Recommended dilution: 1/200-2000. (Gel: 12%SDS-PAGE Lane1: Human fetal brain tissue lysate Lane2: HT-29 cell lysate Lane3: HepG2 cell lysate Lysates: 30 ug per lane Primary antibody: 1/220 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BID antibody

  Rat Monoclonal anti-BID Antibody  
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-BID antibody (clone 1H11). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection. Rat IgG1 1H11 Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BID antibody

  BID mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID. Mouse IgG2a OTI2B6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

  BID mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)  
Anti-BID mouse monoclonal antibody (TA506759) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID(RC207261). Mouse IgG2b OTI2H9 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BID antibody

  BID mouse monoclonal antibody, clone OTI3E6 (formerly 3E6)  
Immunofluorescent staining of HeLa cells using anti-BID mouse monoclonal antibody (TA506763). Mouse IgG2a OTI3E6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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