Biglycan antibody

Principal name

Biglycan antibody

Alternative names for Biglycan antibody

Bone/cartilage proteoglycan I, PG-S1, PGS1, BGN, SLRR1A, Small Leucine-rich Protein 1A

SwissProt ID

O02678 (Canfa), P21809 (Bovin), P21810 (Human), P28653 (Mouse), P47853 (Rat)

Gene ID

633 (BGN), 12111 (Bgn), 25181

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), African clawed frog

Available hosts

Sheep, Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Biglycan antibody

Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

General readings

Babelova A. et al. (2009) J Biol Chem. 284:24035-24048.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Biglycan (C-term) antibody

TA302434 (0.1µg/ml) staining of Rat Skin lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Biglycan antibody

Western blot analysis of extracts of various cell lines, using BGN antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Biglycan antibody

BGN antibody - middle region (ARP56333_P050) validated by WB using Equine Cartilage Explants at 1:1000. Rabbit IgG Purified Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

Biglycan antibody

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: (BGN Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Biglycan antibody

ELISA: Biglycan Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Biglycan (Center) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with BGN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

Biglycan (N-term) antibody

Biglycan Sheep Purified Hu E
1 ml / €460.00
  Acris Antibodies GmbH

Biglycan antibody

Western Blot analysis of BGN expression in transfected 293T cell line (H00000633-T01) by BGN MaxPab polyclonal antibody.

Lane 1: BGN transfected lysate(41.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Biglycan antibody

Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.

Lane 1: BGN transfected lysate(41.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4E1-1G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Biglycan antibody

Western Blot analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M02), clone 2E6-D1.

Lane 1: BGN transfected lysate(41.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2E6-D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Biglycan antibody

BGN polyclonal antibody ( Cat # PAB7149 ) ( 0.1 µg/ml ) staining of rat skin lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Biglycan antibody

Equine Cartilage Explants; BGN antibody - middle region (ARP56333_P050) validated by WB using Equine Cartilage Explants at 1:1000.; BGN antibody - middle region (ARP56333_P050) in Equine Cartilage Explants cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

BGN - human gene knockout kit via CRISPR

BGN - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Bgn - mouse gene knockout kit via CRISPR

Bgn - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Biglycan

Biglycan Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

Biglycan (38-368, His-tag)

Biglycan Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Biglycan (38-368, His-tag)

Biglycan Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Biglycan

Biglycan Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Biglycan

Biglycan Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

BGN (Human) IP-WB Antibody Pair

BGN (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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