BIN1 / AMPHL antibody

Principal name

BIN1 / AMPHL antibody

Alternative names for BIN1 / AMPHL antibody

Bridging integrator 1, Amphiphysin-like protein, Amphiphysin II, Myc box-dependent-interacting protein 1, Box-dependent myc-interacting protein 1

SwissProt ID

O00499 (Human), O08539 (Mouse), O08839 (Rat)

Gene ID

274 (BIN1), 30948 (Bin1), 117028

Available reactivities

Hu (Human), Chk (Chicken), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of BIN1 / AMPHL antibody

Kit Component:
- KN302168G1, Bin1 gRNA vector 1 in pCas-Guide vector
- KN302168G2, Bin1 gRNA vector 2 in pCas-Guide vector
- KN302168D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Ghaneie A, Zemba-Palko V, Itoh H, Itoh K, Sakamuro D, Nakamura S, Soler AP, Prendergast GC.
Bin1 attenuation in breast cancer is correlated to nodal metastasis and reduced survival.
Cancer Biol Ther. 2007 Feb;6(2):192-4. Epub 2007 Feb 7.
PMID: 17218774

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BIN1 / AMPHL antibody

  Goat Polyclonal Antibody against BIN1  
TA303366 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Rabbit polyclonal anti-Bin1 antibody  
Western blot analysis of BIN-1 expression in Jurkat cells treated with (Lane 2) or without (Lane 1) camptothecin. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Mouse monoclonal anti-BIN1 antibody  
Western Blot of Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 µg per lane. Primary antibody: BIN-1 antibody (Exon 12A specific) at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Mouse 12A Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Mouse monoclonal anti-BIN1 antibody  
Western Blot of Mouse Anti-BIN1 antibody. Lane 1: C2C12 during growth. Lane 2: C2C12 during differentiation. Load: 35 µg per lane. Primary antibody: BIN1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~55 kDa for BIN-1. Other band(s): non-specifics. Mouse 99F Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Mouse monoclonal anti-BIN1 antibody  
Western Blot of Mouse Anti-BIN-1 antibody. Lane 1: Keratinocyte derived from Bin-1 wild type. Lane 2: Keratinocyte derived from Bin-1 null mice. Load: 35 µg per lane. Primary antibody: BIN-1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20 (PBS/T ) overnight at 4°C. Bin1 isoforms (+exon13, -exon13). *indicates non-specific signal. Mouse 2F11 Purified Hu, Ms E, IP, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Mouse monoclonal anti-BIN1 antibody  
Western Blot of Anti-BIN1 Antibody. Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 µg per lane. Primary antibody: BIN1 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band(s): non-specific. Mouse 99D Purified Hu, Ms E, F, IP, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Rabbit anti-BIN1 Polyclonal Antibody  
Western blot analysis of extracts of SW620 cell line, using BIN1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

  Mouse Monoclonal anti-Bin1 Antibody  
Human skeletal muscle cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Bin1 (clone 99D) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2b 99D Purified Hu E, F, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Lane 1: Rat Brain Tissue Lysate. 
Lane 2: Rat Skeletal Muscle Tissue Lysate. 
Lane 3: Rat Heart Tissue Lysate.
Lane 4: Rat Kidney Tissue Lysate. Mouse IgG2b BN-1 Purified Chk, Hu, Ms, Por, Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

BIN1 - human gene knockout kit via CRISPR

BIN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BIN1 - human gene knockout kit via CRISPR

BIN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BIN1 - human gene knockout kit via CRISPR

BIN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bin1 - mouse gene knockout kit via CRISPR

Bin1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Bin1 - mouse gene knockout kit via CRISPR

Bin1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bin1 - mouse gene knockout kit via CRISPR

Bin1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bin1 - mouse gene knockout kit via CRISPR

Bin1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BIN1 / AMPHL (transcript variant 9)

  Recombinant protein of human bridging integrator 1 (BIN1), transcript variant 9  
BIN1 / AMPHL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BIN1 / AMPHL (transcript variant 2)

  Recombinant protein of human bridging integrator 1 (BIN1), transcript variant 2  
BIN1 / AMPHL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BIN1 / AMPHL (transcript variant 8)

  Recombinant protein of human bridging integrator 1 (BIN1), transcript variant 8  
BIN1 / AMPHL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BIN1 / AMPHL (1-439, His-tag)

BIN1 / AMPHL Human Purified > 90 % by SDS – PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

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