BIN1 / AMPHL antibody

Principal name

BIN1 / AMPHL antibody

Alternative names for BIN1 / AMPHL antibody

Bridging integrator 1, Amphiphysin-like protein, Amphiphysin II, Myc box-dependent-interacting protein 1, Box-dependent myc-interacting protein 1

SwissProt ID

O00499 (Human), O08539 (Mouse), O08839 (Rat)

Gene ID

274 (BIN1), 30948 (Bin1), 2748280 (Q739J0)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Por (Porcine), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of BIN1 / AMPHL antibody

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq]

General readings

Ghaneie A, Zemba-Palko V, Itoh H, Itoh K, Sakamuro D, Nakamura S, Soler AP, Prendergast GC.
Bin1 attenuation in breast cancer is correlated to nodal metastasis and reduced survival.
Cancer Biol Ther. 2007 Feb;6(2):192-4. Epub 2007 Feb 7.
PMID: 17218774

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BIN1 / AMPHL antibody

TA303366 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Western blot analysis of BIN-1 expression in Jurkat cells treated with (Lane 2) or without (Lane 1) camptothecin. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Western Blot of Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 µg per lane. Primary antibody: BIN-1 antibody (Exon 12A specific) at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Mouse 12A Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Western Blot of Mouse Anti-BIN1 antibody. Lane 1: C2C12 during growth. Lane 2: C2C12 during differentiation. Load: 35 µg per lane. Primary antibody: BIN1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~55 kDa for BIN-1. Other band(s): non-specifics. Mouse 99F Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Western Blot of Mouse Anti-BIN-1 antibody. Lane 1: Keratinocyte derived from Bin-1 wild type. Lane 2: Keratinocyte derived from Bin-1 null mice. Load: 35 µg per lane. Primary antibody: BIN-1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20 (PBS/T ) overnight at 4°C. Bin1 isoforms (+exon13, -exon13). *indicates non-specific signal. Mouse 2F11 Purified Hu, Ms E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Western Blot of Anti-BIN1 Antibody. Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 µg per lane. Primary antibody: BIN1 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band(s): non-specific. Mouse 99D Purified Hu, Ms E, F, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Western blot analysis of extracts of SW620 cell line, using BIN1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

BIN1 / AMPHL antibody

Lane 1: Rat Brain Tissue Lysate. 
Lane 2: Rat Skeletal Muscle Tissue Lysate. 
Lane 3: Rat Heart Tissue Lysate.
Lane 4: Rat Kidney Tissue Lysate. Mouse IgG2b BN-1 Purified Chk, Hu, Ms, Por, Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

BIN1 / AMPHL control peptide

BIN1 / AMPHL Purified
50 µg / €190.00
  Acris Antibodies GmbH

BIN1 / AMPHL antibody

Western Blot analysis of BIN1 expression in transfected 293T cell line (H00000274-T01) by BIN1 MaxPab polyclonal antibody.

Lane 1: BIN1 transfected lysate(53.02 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

Western Blot analysis of BIN1 expression in transfected 293T cell line (H00000274-T02) by BIN1 MaxPab polyclonal antibody.

Lane 1: BIN1 transfected lysate(53.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

Western Blot analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M01), clone 1H1.

Lane 1: BIN1 transfected lysate(53 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1H1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

Western Blot analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M03), clone 2C7.

Lane 1: BIN1 transfected lysate(53 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 2C7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

BIN1 / AMPHL Mouse IgG2b BN-1 Chk, Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

BIN1 polyclonal antibody ( Cat # PAB7487 ) ( 0.1 µg/ml ) staining of human skeletal muscle lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

Western blot analysis of Bin1 expression in Jurkat cells treated with ( Lane 2 ) or without ( Lane 1 ) camptothecin. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

BIN1 / AMPHL antibody

BIN1 / AMPHL Mouse IgG2b BN-1 Ascites Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €290.00
  Boster Immunoleader

BIN1 / AMPHL antibody

Western Blot: BIN1 Antibody [NBP1-46170]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-Bin1 antibody  used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  Bin1 was also immunoprecipitated by rabbit anti-Bin1 antibody NBP1-46169, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BIN1 / AMPHL (transcript variant 9)

BIN1 / AMPHL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BIN1 / AMPHL (transcript variant 2)

BIN1 / AMPHL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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