Biotin antibody

Principal name

Biotin antibody

Alternative names for Biotin antibody

Vitamin B7, Vitamin H

Available reactivities

Hu (Human)

Available hosts

Goat, Mouse, Sheep, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunodiffusion (ID), Immunoprecipitation (IP), Dot blot (Dot)

Background of Biotin antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

General readings

General / background references:Titus JA et al. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Methods 50:193-204 (1982). PubMed PMID: 6806389

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Biotin antibody

Biotin Mouse IgG1 B25 (BGN/09/6225) Purified E
0.2 mg / €290.00
  Acris Antibodies GmbH

Biotin antibody

Antibody Sensitivity: Biotinylated Molecular Weight Marker (Biorad) was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with Biotin antibody AM20209PU-N  (Clone 2H2) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: 200nl 
Lane 2: 100nl
Lane 3: 50nl
Lane 4: 20nl
Lane 5: 10nl
Lane 6: 5nl
Lane 7: 2nl Mouse IgG1 2H2 Purified Hu E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Mouse IgG1 B-E24 Azide Free Hu F, P
0.2 mg / €270.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Mouse IgG3 BIO-8 Purified C
1 ml / €420.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Goat Serum Dot, E, ID, IP, WB
2 ml / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG Serum Dot, E, IP, WB
2 ml / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Goat IgG DTAF F, ICC/IF
20 mg / €3,300.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG FITC C, F, ICC/IF
20 mg / €3,300.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG Texas Red E, F
20 mg / €3,300.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Goat IgG PE F
1 ml / €510.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Goat IgG TRITC F
1 mg / €510.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG Rhodamine F, ICC/IF
0.1 ml / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG AP C, Dot, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG FITC F, ICC/IF
0.1 ml / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG HRP C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG Aff - Purified E, ID, IP, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG PE F, ICC/IF
0.1 ml / €440.00
  Acris Antibodies GmbH

Biotin antibody

Biotin Rabbit IgG Texas Red F, ICC/IF
0.1 ml / €440.00
  Acris Antibodies GmbH

Biotin (F(ab')2 fragment) antibody

Biotin Goat IgG AP C, Dot, E, WB
1 mg / €510.00
  Acris Antibodies GmbH

Biotin (F(ab')2 fragment) antibody

Biotin Goat IgG FITC F, ICC/IF
1 mg / €510.00
  Acris Antibodies GmbH

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