BLK antibody

Principal name

BLK antibody

Alternative names for BLK antibody

B lymphocyte kinase, p55-BLK, p55BLK, Tyrosine-protein kinase BLK

SwissProt ID

P16277 (Mouse), P51451 (Human)

Gene ID

640 (BLK), 5778640 (IPR006726)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BLK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

General readings

Dymecki. et al. (1990) Science. 247:332-6.Tatosyan, A.G. et al. (2000) Biochemistry. 65:49-58

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BLK (N-term) antibody

Western blot analysis of anti-BLK Pab in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BLK (N-term) antibody

(0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BLK antibody

WB Suggested Anti-BLK Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BLK antibody

Immunohistochemical analysis of paraffin-embedded human breast tissue (A), lymph tissue (B) and skin carcinoma (C), showing membrane localization using BLK antibody Cat.-No AM06135SU-N with DAB staining. Mouse IgG2a 9D10D1 Ascites Hu P
0.1 ml / €440.00
  Acris Antibodies GmbH

BLK antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), lymph tissue (B) and skin carcinoma (C), showing membrane localization using BLK antibody Cat.-No AM06138SU-N with DAB staining. Mouse IgG1 9D10B7H6 Ascites Hu E, P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

BLK antibody

Immunofluorescence analysis of Hela cells using BLK antibody Cat.-No AM06549SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled Mouse IgG1 1E6 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

BLK antibody

Western blot analysis of BLK expression in mouse kidney tissue lysate. Rabbit Aff - Purified Ms WB
0.1 mg / €410.00
  Acris Antibodies GmbH

BLK pTyr501 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody AP55751PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
0.1 ml / €340.00
  Acris Antibodies GmbH

BLK pTyr501 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody AP55751PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
50 µl / €270.00
  Acris Antibodies GmbH

BLK antibody

Western Blot analysis of BLK expression in transfected 293T cell line (H00000640-T01) by BLK MaxPab polyclonal antibody.

Lane 1: BLK transfected lysate(55.55 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BLK antibody

BLK Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

BLK antibody

BLK Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

BLK antibody

Western Blot analysis of BLK expression in transfected 293T cell line (H00000640-T01) by BLK MaxPab polyclonal antibody.

Lane 1: BLK transfected lysate(57.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BLK antibody

Western Blot analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M01), clone 3E5-3A8.

Lane 1: BLK transfected lysate(57.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 3E5-3A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BLK antibody

BLK monoclonal antibody (M02), clone 7A12. Western Blot analysis of BLK expression in PC-12(Cat # L012V1 ). Mouse IgG1 7A12 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BLK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BLK polyclonal antibody ( Cat # PAB3417 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BLK pTyr501 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BLK (Phospho-Tyr501) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

BLK pTyr501 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BLK (Phospho-Tyr501) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

BLK antibody

Human HepG2; WB Suggested Anti-BLK Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; BLK antibody - middle region (ARP46061_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

BLK antibody

Immunohistochemistry: BLK Antibody (9D10D1) [NB110-60498] - Figure 1: Immunohistochemical analysis of paraffin-embedded Human breast tissue, lymph tissue and skin carcinoma tissue, showing membrane localization using BLK antibody with DAB staining. Mouse IgG2a 9D10D1 Ascites Hu C, E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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