BLM antibody

Principal name

BLM antibody

Alternative names for BLM antibody

Bloom syndrome protein, RecQ protein-like 3, DNA helicase, RecQ-like type 2, RECQ2, RECQL3

SwissProt ID

O88700 (Mouse), P54132 (Human), Q9I920 (Chick)

Gene ID

12144 (Blm), 641 (BLM)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BLM antibody

The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44% amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions.
The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process.
Bloom syndrome cells show marked genomic instability; in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.

General readings

Ababou M, Dutertre S, L

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BLM (C-term) antibody


Staining of nuclear HeLa lysate using BLM Antibody Cat.-No AP32057PU-N at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Hu, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

BLM antibody

Blooms Syndrome Protein Blm [phospho Thr99] Antibody - Western blot analysis of Blooms Syndrome Protein Blm phosphorylated at Thr99 in MDA-MB-231 cells A) not treated and B) treated with 1uM camptothecin using TA309588.  Image courtesy of Dr. Keli Agama. Rabbit Purified Hu WB
50 µl / €379.00
  OriGene Technologies, Inc.

BLM (1319-1335) antibody

Staining Blooms Syndrome Protein Blm antibody in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

BLM pThr99 antibody

Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody AP55713PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

BLM pThr99 antibody

Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody AP55713PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P, WB
50 µl / €270.00
  Acris Antibodies GmbH

BLM antibody

Detection of human BLM in whole cell lysates of HeLa and 293T cells as indicated by Western blotting using Rabbit anti Human BLM (SP2060P) Rabbit Aff - Purified Hu IP, WB
50 µg / €330.00
  Acris Antibodies GmbH

BLM antibody

BLM Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

BLM antibody

BLM Mouse IgG1 1E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BLM (1218-1233) antibody

The BCN antibody recognizes BLM. Whole cell BLM 160 kDa l 4), extracts (30 μg) from wt cells and from BLM patientderived cells where fractionated in a 6% SDS-PAGE, transferred to nitrocellulose membrane, and probed with the anti-BLM BCN antibody diluted 1:100. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. Rabbit IgG Aff - Purified Hu WB
50 µl / €300.00
  Antibody BCN

BLM pThr99 antibody

Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

BLM pThr99 antibody

Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

BLM antibody

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using Bloom Syndrome (Ab-99) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

BLM antibody

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using Bloom Syndrome (Ab-99) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

BLM (1425-1477) antibody

Western Blot: BLM Antibody [NB100-214] - Western blot analysis of human BLM, using NB100-214. Sample: whole cell lysate from 293T cells (35 ug). Rabbit Aff - Purified Hu IP, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

BLM (100-150) antibody

Western Blot: BLM Antibody [NB100-324] - Detection of Human BLM (NB100-324) by Western Blot. Sample: Whole cell lysate from 293T cells (35 ug for WB). Goat Aff - Purified Hu IP, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

BLM (Super Novus Pack) antibody

Western Blot: BLM Antibodies SuperNovus Pack [NB100-918] - Western blot analysis of human BLM, using NB100-214. Sample: whole cell lysate from 293T cells (35 ug). Aff - Purified Hu IP, WB
2 vials / €510.00
  Novus Biologicals Inc.

BLM (1375-1416) antibody

Western Blot and Immunoprecipitation: BLM Antibody [NB100-1590] - Whole cell lysate from mouse NIH3T3 cells. BLM was also immunoprecipitated using rabbit anti-BLM antibodies NB100-1588 and NB100-1589. Rabbit Aff - Purified Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

BLM antibody

BLM Rabbit Aff - Purified Hu WB
25 µl / €170.00
  Novus Biologicals Inc.

BLM antibody

Immunohistochemistry-Paraffin: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

BLM antibody pack

BLM antibody pack Ms IP, WB
3 vials / €680.00
  Novus Biologicals Inc.

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