BMF antibody

Principal name

BMF antibody

Alternative names for BMF antibody

Bcl-2-modifying factor

SwissProt ID

Q8K589 (Rat), Q91ZE9 (Mouse), Q96LC9 (Human)

Gene ID

90427 (BMF), 171543 (Bmf)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Rat, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of BMF antibody

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell\'s actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

General readings

Morales AA., et al. (2004). Leukemia. 18(1):41-7.
Chen L., et al. (2005). Mol Cell. 17(3):393-403.

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 31 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BMF antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807459)(1:150) Mouse IgG2b OTI6F3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807461)(1:150) Mouse IgG1 OTI9B7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF (C-term) antibody

Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 ug/mL. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMF (N-term) antibody

Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Bmf antibody at 2 ug /ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMF antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807460) Mouse IgG1 OTI3E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF (RC209733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMF. Mouse IgG1 OTI5H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF (RC209733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMF. Mouse IgG2a OTI7B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF (RC209733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMF.(1:2000) Mouse IgG2a OTI4F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807685)(1:150) Mouse IgG1 OTI3H5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BMF (BH3 Domain) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BMF antibody

AP26342PU-N: Western blot analysis of BMF expression in Jurkat cell lysate (Lane 1 & 2), 3T3 cell lysate (Lane 3) and in Rat kidney tissue lysate (Lane 4). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

BMF Control Peptide

BMF
50 µg / €160.00
  Acris Antibodies GmbH

BMF Control Peptide

BMF
50 µg / €160.00
  Acris Antibodies GmbH

BMF (N-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Bmf (SP1414P) Rabbit Purified Hu, Ms ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

BMF antibody

BMF Rat IgG1 12E10 Purified Hu, Ms F, IP, WB
0.1 mg / €730.00
  Abnova Taiwan Corp.

BMF antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the BMF polyclonal antibody ( Cat # PAB2443 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

BMF antibody

Immunofluorescence of BMF in human kidney tissue with BMF polyclonal antibody ( Cat # PAB13104 ) at 10 µg/ml. Rabbit Hu C, ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BMF antibody

Immunocytochemistry of BMF in HeLa cells with BMF polyclonal antibody ( Cat # PAB13105 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BMF (transcript variant 2)

BMF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Items 1 to 20 of 31 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn