BMF antibody

Principal name

BMF antibody

Alternative names for BMF antibody

Bcl-2-modifying factor

SwissProt ID

Q8K589 (Rat), Q91ZE9 (Mouse), Q96LC9 (Human)

Gene ID

90427 (BMF), 171543 (Bmf)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Rat, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of BMF antibody

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell\'s actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

General readings

Morales AA., et al. (2004). Leukemia. 18(1):41-7.
Chen L., et al. (2005). Mol Cell. 17(3):393-403.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BMF antibody

  BMF mouse monoclonal antibody, clone OTI6F3 (formerly 6F3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807459)(1:150) Mouse IgG2b OTI6F3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

  BMF mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807461)(1:150) Mouse IgG1 OTI9B7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF (C-term) antibody

  Rabbit Polyclonal Bmf Antibody  
Western blot analysis of Bmf expression in HepG2 cell lysate with Bmf antibody at (A) 2.5 and (B) 5 ug/mL. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMF (N-term) antibody

  Rabbit Polyclonal Bmf Antibody  
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Bmf antibody at 2 ug /ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMF antibody

  BMF mouse monoclonal antibody, clone OTI3E5 (formerly 3E5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807460) Mouse IgG1 OTI3E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

  BMF mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF (RC209733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMF. Mouse IgG1 OTI5H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

  BMF mouse monoclonal antibody, clone OTI7B3 (formerly 7B3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF (RC209733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMF. Mouse IgG2a OTI7B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

  BMF mouse monoclonal antibody, clone OTI4F4 (formerly 4F4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMF (RC209733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMF.(1:2000) Mouse IgG2a OTI4F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

  BMF mouse monoclonal antibody, clone OTI3H5 (formerly 3H5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807685)(1:150) Mouse IgG1 OTI3H5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BMF antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BMF (BH3 Domain) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BMF antibody

AP26342PU-N: Western blot analysis of BMF expression in Jurkat cell lysate (Lane 1 & 2), 3T3 cell lysate (Lane 3) and in Rat kidney tissue lysate (Lane 4). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

BMF (N-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Bmf (SP1414P) Rabbit Purified Hu, Ms ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Bmf - mouse gene knockout kit via CRISPR

Bmf - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Bmf - mouse gene knockout kit via CRISPR

Bmf - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bmf - mouse gene knockout kit via CRISPR

Bmf - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Bmf - mouse gene knockout kit via CRISPR

Bmf - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BMF (transcript variant 2)

  Recombinant protein of human Bcl2 modifying factor (BMF), transcript variant 2  
BMF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BMF (full length, N-term HIS tag)

  Recombinant protein of human Bcl2 modifying factor (BMF), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
BMF Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

BMF (1-129, T7-tag)

BMF Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

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