BMI-1 / RNF51 antibody

Principal name

BMI-1 / RNF51 antibody

Alternative names for BMI-1 / RNF51 antibody

BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

SwissProt ID

P25916 (Mouse), P35226 (Human), Q32KX7 (Bovin), Q5SDR3 (Chick)

Gene ID

648 (BMI1), 100532731 (COMMD3-BMI1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Mky (Monkey), Rb (Rabbit), Ze (Zebrafish), Frog, Chimp (Chimpanzee), Prim (Primate), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of BMI-1 / RNF51 antibody

BMI1 polycomb ring finger oncogene, also known as BMI1, is a protein which in humans is encoded by the BMI1 gene. BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 is rapidly recruited to sites of DNA damage and it sustains for over than 8h. Loss of BMI1 leads to radiation sensitive and impaired repair of DNA double-strand breaks by homologous recombination. Bmi1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells.
Bmi1 is also thought to inhibit ageing in neurons through the suppression of p53. In primary and tumor cells, nuclear BMI1 shows a fine-grain distribution over chromatin, usually dense in interphase nuclei and significantly weaker along mitotic chromosomes.
In addition, BMI1 preferentially associates with several distinct heterochromatic domains in tumor cell lines. Bmi1 seems to play an important role in several types of cancer, such as bladder, skin, prostate, breast, ovarian, colorectal as well as hematological malignancies. Its amplification and overexpression is especially pronounced in mantle cell lymphomas.
In both primary and tumor cell lines a marked cell cycle regulation of Pc-G-chromatin interaction is observed: nuclear BMI1-staining dissipates in late S phase and is reestablished early in G1-phase. Chromatin-association of BMI1 inversely correlates with its phosphorylation status in a cell cycle-dependent fashion: at G1/S, hypophosphorylated. BMI1 is specifically retained in the chromatin-associated nuclear protein fraction, whereas during G2/M, phosphorylated BMI1 is not chromatin bound.

General readings

Voncken JW et al. MAPKAP kinase 3pK phosphorylates and regulates chromatin association of the polycomb group protein Bmi1. J Biol Chem 280:5178-87 (2005). PubMed: 15563468.
Obuse C et al. Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase. Genes Cells 9:105-20 (2004). PubMed: 15009096.
Kim JH et al. The Bmi-1 oncoprotein is overexpressed in human colorectal cancer and correlates with the reduced p16INK4a/p14ARF proteins. Cancer Lett 203:217-24 (2004). PubMed: 14732230.
Search PubMed (MEDLINE) for references to Bmi1

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BMI-1 / RNF51 antibody

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining. Mouse IgG1 3E3 Ascites Hu E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

BMI-1 / RNF51 antibody

Detection of Bmi1 protein in skeletal muscle Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 (Center) antibody

Western blot analysis of BMI-1 in K562 cell lysate with BMI-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

Western Blot: Bmi1 Antibody (LLBmi1-1) - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using anti-Bmi1. Mouse IgG2b LLBmi1-1 Purified Hu, Ms C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 (252-264) antibody

WB using Anti-Bmi1 antibody shows detection of a band ~37 kDa corresponding to human Bmi1 (arrowhead).Approximately 20 µg of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. The primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk.  The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature. Goat Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

Western blot analysis of BMI1 Antibody (Cat. #TA325069) in MCF7 cell line lysates (35ug/lane). BMI1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

Western blot analysis of extracts of K562 cells , using BMI1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

Western blot analysis of extracts of U-937 cell line, using BMI1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

WB Suggested Anti-PCGF4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: A172 cell lysate; BMI1 is supported by BioGPS gene expression data to be expressed in A172 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against mouse BMI1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,100. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

BMI-1 / RNF51 antibody

BMI-1 / RNF51 Mouse IgG1 229F6 Purified Hu, Ms, Rb, Rt C, IP, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

BMI-1 / RNF51 antibody

Immunocytochemical staining of K562 cells with Rabbit anti Human BMI-1 (AP05603PU-N) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

BMI-1 / RNF51 antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Can, Chk, Fe, Frog, Hu, Mky, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BMI-1 / RNF51 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

BMI-1 / RNF51 Control Peptide

BMI-1 / RNF51
50 µg / €160.00
  Acris Antibodies GmbH

BMI-1 / RNF51 antibody

Western Blot analysis of BMI1 expression in transfected 293T cell line (H00000648-T02) by BMI1 MaxPab polyclonal antibody.

Lane 1: BMI1 transfected lysate(36.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BMI-1 / RNF51 antibody

Western Blot analysis of PCGF4 expression in transfected 293T cell line by PCGF4 monoclonal antibody (M02), clone 4E10-1C5.

Lane 1: PCGF4 transfected lysate(36.9 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 4E10-1C5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BMI-1 / RNF51 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with BMI1 polyclonal antibody ( Cat # PAB1687 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

BMI-1 / RNF51 antibody

Western blot using BMI1 polyclonal antibody ( Cat # PAB9982 ) shows detection of a band ~37 kDa corresponding to human BMI1 ( arrowhead ).
Approximately 20 µg of a U-2 OS whole cell lysate ( bone osteosarcoma ) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,000 in PBS containing 1% nonfat dry milk.
The membrane was washed and reacted with a 1 : 20,000 dilution of IRDyeTM800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Bov, Chimp, Chk, Hu, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

BMI-1 / RNF51 antibody

Immunocytochemistry of BMI1 in K-562 cells with BMI1 polyclonal antibody ( Cat # PAB12958 ) at 10 µg/ml. Rabbit Hu, Ms, Rt ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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