BOLL / BOULE antibody

Principal name

BOLL / BOULE antibody

Alternative names for BOLL / BOULE antibody

Protein boule-like

SwissProt ID

Q8N9W6 (Human), Q924M5 (Mouse)

Gene ID

66037 (BOLL)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of BOLL / BOULE antibody

**Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognize native protein in any application. This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

General readings

Xu EY, Moore FL, Pera RA. A gene family required for human germ cell development evolved from an ancient meiotic gene conserved in metazoans. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7414-9. PMID: 11390979

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BOLL / BOULE antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BOLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804110) Mouse IgG1 OTI2D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BOLL / BOULE antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BOLL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804111) Mouse IgG1 OTI4C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BOLL / BOULE antibody

(4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Bov, Can, Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

BOLL / BOULE antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/25-50 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue  using BOLL antibody at dilution 1/10, on the right is immunohistochemistry of paraffin-embedded Human lung cancer tissue using BOLL antibody at dilution 1/10. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BOLL / BOULE antibody

WB Suggested Anti-BOLL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

BOLL / BOULE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BOLL (RC207436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BOLL. Mouse IgG1 OTI5A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BOLL / BOULE (N-term) antibody

BOLL Antibody (N-term) (Cat. #AP50383PU-N) western blot analysis in A549 cell line lysates (35µg/lane).This demonstrates the BOLL antibody detected the BOLL protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BOLL / BOULE antibody

Immunoperoxidase of purified MaxPab antibody to BOLL on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

BOLL MaxPab rabbit polyclonal antibody. Western Blot analysis of BOLL expression in mouse testis. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

BOLL MaxPab rabbit polyclonal antibody. Western Blot analysis of BOLL expression in mouse testis. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Western Blot analysis of BOLL expression in transfected 293T cell line by BOLL monoclonal antibody (M01), clone 1F3.

Lane 1: BOLL transfected lysate(31.301 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1F3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Western Blot analysis of BOLL expression in transfected 293T cell line by BOLL monoclonal antibody (M02), clone 1E3.

Lane 1: BOLL transfected lysate(31.301 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Immunoperoxidase of monoclonal antibody to BOLL on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] Mouse IgG2a 3E10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Western Blot analysis of BOLL expression in transfected 293T cell line by BOLL monoclonal antibody (M04), clone 1G8.

Lane 1: BOLL transfected lysate(31.301 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

BOLL monoclonal antibody (M05), clone 5B8 Western Blot analysis of BOLL expression in SW-13 ( Cat # L005V1 ). Mouse IgG2a 5B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Immunoperoxidase of monoclonal antibody to BOLL on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.2 ug/ml] Mouse IgG2a 2E8 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1E1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 5H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

BOLL monoclonal antibody (M09), clone 1A7 Western Blot analysis of BOLL expression in Hela NE ( Cat # L013V3 ). Mouse IgG2a 1A7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BOLL / BOULE antibody

BOLL monoclonal antibody (M10), clone 1A2. Western Blot analysis of BOLL expression in H9c2(2-1). (Isoform : 37.540 kDa) Mouse IgG2a 1A2 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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