Bone sialoprotein 2 antibody

Principal name

Bone sialoprotein 2 antibody

Alternative names for Bone sialoprotein 2 antibody

Bone sialoprotein II, BSP II, IBSP, BNSP, Cell-binding sialoprotein, Integrin-binding sialoprotein

SwissProt ID

P13839 (Rat), P21815 (Human), Q28862 (Bovin), Q61711 (Mouse)

Gene ID

3381 (IBSP)

Available reactivities

Hu (Human), Por (Porcine), Rb (Rabbit), Rt (Rat), Ms (Mouse), Can (Canine)

Available hosts

Rabbit, Goat, Chicken, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C)

Background of Bone sialoprotein 2 antibody

IBSP (integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

General readings

Barnes,G.L., et al., (2003) Cancer Res. 63 (10), 2631-2637

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bone sialoprotein 2 antibody

WB Suggested Anti-IBSP Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Bone sialoprotein 2 (native) antibody

Immunohistochemistry image staining of BSP in Paraffin Section of Human Tissues. Sections were incubated with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S (1/2000) and detected using HRP conjugated secondary antibody and ABC Vectastain Elite Kit. DAB was used as the Chromogen. The sections were counterstained with Harris' Hematoxylin.
Sections of B: Embryonic Tibia
Sections of E: Primary breast Carcinoma
Sections of G: Bone metastasis from the same patient were stained with this antibody.
Inserted within B and E are Control Sections incubated with Mouse IgG. Scale bar = 20 µm.
Gogan G et al. (2004) Connect Tissue Res 45(1):60-71 Mouse IgG1 ID1.2 Purified Hu C, E, ICC/IF, P, R, WB
0.1 mg / €510.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (native) antibody

Immunohistochemistry image staining of BSP in Paraffin Section of Human Tissues. Sections were incubated with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S (1/2000) and detected using HRP conjugated secondary antibody and ABC Vectastain Elite Kit. DAB was used as the Chromogen. The sections were counterstained with Harris' Hematoxylin.
Sections of B: Embryonic Tibia
Sections of E: Primary breast Carcinoma
Sections of G: Bone metastasis from the same patient were stained with this antibody.
Inserted within B and E are Control Sections incubated with Mouse IgG. Scale bar = 20 µm.
Gogan G et al. (2004) Connect Tissue Res 45(1):60-71 Mouse IgG1 ID1.2 Purified Hu, Por, Rb, Rt C, E, ICC/IF, P, R, WB
10 µg / €150.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (CD-BSP 108-122) antibody

Bone sialoprotein 2 Mouse IgG1 243 8B1 Purified Hu E
0.1 mg / €510.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (CD-BSP 108-122) antibody

Bone sialoprotein 2 Mouse IgG1 243 8B1 Purified Hu E
10 µg / €150.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (CD-BSP 108-122) antibody

ELISA Standard curve showing measurement of Human BSP in a Sandwich immunoassay using AM20240PU as a Capture antibody and AM02057PU as a Detection antibody. OD was determined at 450 nm. 
The shown standard curve is for demonstration purposes only and can be influenced by Buffer, incubation conditions or dilution of the conjugate. Rat IgG1 IDBSP5 Purified Hu E, WB
0.1 ml / €510.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (CD-BSP 108-122) antibody

ELISA Standard curve showing measurement of Human BSP in a Sandwich immunoassay using AM20240PU as a Capture antibody and AM02057PU as a Detection antibody. OD was determined at 450 nm. 
The shown standard curve is for demonstration purposes only and can be influenced by Buffer, incubation conditions or dilution of the conjugate. Rat IgG1 IDBSP5 Purified Hu E, WB
20 µl / €200.00
  Acris Antibodies GmbH

Bone sialoprotein 2 antibody

Bone sialoprotein 2 Goat Serum Hu E, R
0.1 ml / €380.00
  Acris Antibodies GmbH

Bone sialoprotein 2 antibody

Bone sialoprotein 2 Goat Serum Hu E, R
20 µl / €150.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (108-122) antibody

Bone sialoprotein 2 Rabbit Serum Hu E, ICC/IF, WB
0.1 ml / €500.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (108-122) antibody

Bone sialoprotein 2 Rabbit Serum Hu E, ICC/IF, WB
20 µl / €170.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (201-211) antibody

Western Blot analysis of BSP expression in different cell lines. 
Cell lysates and recombinant BSP were separated by SDS-PAGE and immunoblotted with AP02051SU-S/N (1/7000). Rabbit Serum Hu E, ICC/IF, WB
0.1 ml / €500.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (201-211) antibody

Western Blot analysis of BSP expression in different cell lines. 
Cell lysates and recombinant BSP were separated by SDS-PAGE and immunoblotted with AP02051SU-S/N (1/7000). Rabbit Serum Hu E, ICC/IF, WB
20 µl / €170.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (262-279) antibody

Bone sialoprotein 2 Rabbit Serum Hu E, ICC/IF
0.1 ml / €500.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (262-279) antibody

Bone sialoprotein 2 Rabbit Serum Hu E, ICC/IF
20 µl / €170.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (284-295) antibody

Bone sialoprotein 2 Rabbit Serum Hu E
0.1 ml / €500.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (284-295) antibody

Bone sialoprotein 2 Rabbit Serum Hu E
20 µl / €170.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (39-47) antibody

Bone sialoprotein 2 Rabbit Serum Hu E, ICC/IF
0.1 ml / €500.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (39-47) antibody

Bone sialoprotein 2 Rabbit Serum Hu E, ICC/IF
20 µl / €170.00
  Acris Antibodies GmbH

Bone sialoprotein 2 (native) antibody

Immunohistochemistry of BSP staining in Paraffin Sections of pancreatic ductal adenocarcinoma. Sections were incubated with BSP antibody Cat.-No AP02055PU (4 µg/ml) and detected using HRP conjugated secondary antibody and DAB as chromogen. The sections were counterstained with Mayer's hematoxylin. 
Image A: AP02055PU stains pancreatic cancer cells. 
Image D: Lymph node metastases of pancreatic cancer cells. 
Scale bar = 50 µm. 
Kayed H et al. (2007) Cancer Lett 245(1-2): 171-183. Chicken Purified Hu E, F, ICC/IF, P, R, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

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