BPI antibody

Principal name

BPI antibody

Alternative names for BPI antibody

CAP57, Bactericidal permeability-increasing protein

SwissProt ID

P17213 (Human)

Gene ID

671 (BPI)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Functional assay (FN), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BPI antibody

The monoclonal antibody 4E3 reacts specifically with full length human natural and recombinant Bactericidal Permeability Increasing protein (BPI). The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airways disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation. Furthermore, a 21 kDa bioactive recombinant fragment of BPI, rBPI21, was shown to confer a survival advantage against invasive pneumococcal disease by binding to the gram-positive bacterial pathogen, pneumolysin. The monoclonal antibody 4E3 recognizes only free BPI and does not interact with BPI that has formed a complex with LPS.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BPI antibody

Western blot analysis of extracts of various cell lines, using BPI antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

BPI antibody

TA348932 (0.3 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BPI antibody

BPI Mouse IgG1 GE 4.90 Purified Hu E, FN
0.1 mg / €490.00
  Acris Antibodies GmbH

BPI antibody

BPI Mouse IgG1 4H5 Purified Hu E
0.1 mg / €490.00
  Acris Antibodies GmbH

BPI antibody

BPI Mouse IgG1 3F9 Purified Hu E
0.1 mg / €490.00
  Acris Antibodies GmbH

BPI antibody

BPI Rabbit IgG Purified E, IP
0.1 mg / €450.00
  Acris Antibodies GmbH

BPI (Center) antibody

Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BPI antibody

BPI Rabbit Purified Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

BPI antibody

BPI Mouse IgG1 4E3 Purified Hu E, F
50 µg / €480.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BPI

BPI Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

BPI

BPI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

BPI overexpression lysate

BPI overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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