BRCA1 / RNF53 antibody

Principal name

BRCA1 / RNF53 antibody

Alternative names for BRCA1 / RNF53 antibody

Breast cancer type 1 susceptibility protein, RING finger protein 53

SwissProt ID

O54952 (Rat), P38398 (Human), P48754 (Mouse), Q864U1 (Bovin), Q95153 (Canfa)

Gene ID

672 (BRCA1), 21933 (Tnfrsf10b), 12189 (Brca1)

Available reactivities

Can (Canine), Dros (Drosophila), Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Chimp (Chimpanzee), Mky (Monkey), Gorilla

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), PLA/Proximity ligation assay (PLA)

Background of BRCA1 / RNF53 antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase; phosphorylated BRCA1 resides in inner chromosomal structure, centrosome, cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein, participate in transcription coupled repair of oxidative DNA damage, X-chromosome inactivation, and can function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA1 protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.

General readings

K Kashima, T Oite, Y Aoki, K Takakuwa, H Aida, H Nagata, M Sekine, HJ Wu, Y Hirai, Y Wada, K Yamamoto, K Hasegawa, T Sonoda, T Maruo, I Nagata, M Ohno, M Suzuki, I Kobayashi, K Kuzuya, T Takahashi, Y Torii, and K Tanaka. Screening of BRCA1 mutation using immunohistochemical staining with C-terminal and N-terminal antibodies in familial ovarian cancers. Jpn J Cancer Res, Apr 2000; 91(4): 399-409.
Fátima H. Vaz, Patrícia M. Machado, Rita D. Brandão, Cátia T. Laranjeira, Joana S. Eugénio, Aires H. Fernandes, and Saudade P. André. Familial Breast/Ovarian Cancer and BRCA1/2 Genetic Screening: The Role of Immunohistochemistry as an Additional Method in the Selection of Patients. J. Histochem. Cytochem., Jul 2007; 10.1369/jhc.7A7209.2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BRCA1 / RNF53 antibody

Detection of BRCA1 in MCF-7 whole cell lysate using anti-BRCA1.  12 minute ECL exposure. Mouse IgG2b MU Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Detection of BRCA1 in U2OS whole cell lysate using anti-BRCA1.  1 minute ECL detection. Mouse IgG3 KEN Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Figure from citation: Co-immunoprecipitation Western
blot analysis of proteins from different cell lines using BRCA1 pulldown and probing with
anti-BRCA1, PALB2, BARD1, and BRCA2 antibodies. View Citation Rabbit IgG Purified Hu P
0.1 mg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BRCA1 (Ab-1423) Antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Western blot analysis of BRCA1 expression in whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Western blot analysis of BRCA1 expression in serum treated COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Western blot analysis of extracts of various cell lines, using BRCA1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

Western Blot: BRCA1 Antibody - Western Blot was performed using affinity purified SEQer BRCA1 antibody, aa(1439-1538) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer BRCA1 antibody, aa(1439-1538) antibody.  Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec).  The protein was detected as represented by the band shown. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

ELISA: BRCA1 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

ELISA: BRCA1 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

ELISA: BRCA1 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

ELISA: BRCA1 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

ELISA: BRCA1 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

BRCA1 / RNF53 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (RC219679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1. Mouse IgG1 OTI5D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BRCA1 / RNF53 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (RC218344, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1. Mouse IgG1 OTI2A5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BRCA1 / RNF53 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (RC218344, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1. Mouse IgG1 OTI1C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BRCA1 / RNF53 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (RC219679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1. Mouse IgG1 OTI6H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BRCA1 / RNF53 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (RC219679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1. Mouse IgG1 OTI7B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

BRCA1 / RNF53 antibody

Western blot analysis of BRCA1 expression in HeLa (left lane) and NIH3T3 (right lane) cell lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

BRCA1 / RNF53 pSer1189 antibody

BRCA1 / RNF53 Rabbit Aff - Purified Hu WB
0.1 mg / €460.00
  Acris Antibodies GmbH

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