BRCA1 / RNF53 antibody

Principal name

BRCA1 / RNF53 antibody

Alternative names for BRCA1 / RNF53 antibody

Breast cancer type 1 susceptibility protein, RING finger protein 53

SwissProt ID

O54952 (Rat), P38398 (Human), P48754 (Mouse), Q864U1 (Bovin), Q95153 (Canfa)

Gene ID

672 (BRCA1), 21933 (Tnfrsf10b), 12189 (Brca1)

Available reactivities

Can (Canine), Dros (Drosophila), Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Chimp (Chimpanzee), Mky (Monkey), Gorilla

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), PLA/Proximity ligation assay (PLA)

Background of BRCA1 / RNF53 antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger protein containing a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms. The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes to the mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes. Unphosphorylated BRCA1 localizes on chromosomes from metaphase through telophase; phosphorylated BRCA1 resides in inner chromosomal structure, centrosome, cleavage furrow during prophase through telophase, and relocalizes to the perinuclear region when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as a tumor suppressor and can function as a secreted growth inhibitory protein, participate in transcription coupled repair of oxidative DNA damage, X-chromosome inactivation, and can function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated by Ets-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination and phosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has been reported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA1 protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.

General readings

K Kashima, T Oite, Y Aoki, K Takakuwa, H Aida, H Nagata, M Sekine, HJ Wu, Y Hirai, Y Wada, K Yamamoto, K Hasegawa, T Sonoda, T Maruo, I Nagata, M Ohno, M Suzuki, I Kobayashi, K Kuzuya, T Takahashi, Y Torii, and K Tanaka. Screening of BRCA1 mutation using immunohistochemical staining with C-terminal and N-terminal antibodies in familial ovarian cancers. Jpn J Cancer Res, Apr 2000; 91(4): 399-409.
Fátima H. Vaz, Patrícia M. Machado, Rita D. Brandão, Cátia T. Laranjeira, Joana S. Eugénio, Aires H. Fernandes, and Saudade P. André. Familial Breast/Ovarian Cancer and BRCA1/2 Genetic Screening: The Role of Immunohistochemistry as an Additional Method in the Selection of Patients. J. Histochem. Cytochem., Jul 2007; 10.1369/jhc.7A7209.2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BRCA1 / RNF53 antibody

BRCA1 in MCF-7 whole cell lysate. 12 minute ECL exposure. Mouse IgG1 24E6 Ascites Hu WB
0.1 ml / €370.00
  Neuromics Antibodies

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