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BRD2 antibody

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Principal name

BRD2 antibody

Alternative names for BRD2 antibody

KIAA9001, RING3, O27.1.1, Bromodomain-containing protein 2

SwissProt ID

P25440 (Human), Q32S26 (Bovin), Q5TJG6 (Canfa), Q6MGA9 (Rat), Q7JJ13 (Mouse)

Gene ID

6046 (BRD2), 14312 (Brd2), 294276

Ncbi ID

NP_005095, 9606, 10090, NP_001106653, NP_001106653.1, NP_005095.1, NP_001191902.1, NM_010238, NM_212495, NM_005104, NM_001113182

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BRD2 antibody

Kit Component:
- KN302252G1, Brd2 gRNA vector 1 in pCas-Guide vector
- KN302252G2, Brd2 gRNA vector 2 in pCas-Guide vector
- KN302252D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Boehm,D., et al. (2013) Cell Cycle 12 (3), 452-462Tang,X., et al. (2013) Mol. Pharmacol. 83 (1), 283-293

ARP34212_P050 - BRD2
Human Jurkat; WB Suggested Anti-BRD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; BRD2 antibody - middle region (ARP34212_P050) in Human Jurkat cells using Western Blot
H00006046-M01 - BRD2
BRD2 monoclonal antibody (M01), clone 3D10. Western Blot analysis of BRD2 expression in Hela NE(Cat # L013V3 ).
H00006046-M01 - BRD2
Detection limit for recombinant GST tagged BRD2 is approximately 0.03ng/ml as a capture antibody.
ARP34211_T100 - BRD2
Human Jurkat; WB Suggested Anti-BRD2 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; BRD2 antibody - C-terminal region (ARP34211_T100) in Human Jurkat cells using Western Blot
PAB3245 - BRD2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BRD2 polyclonal antibody ( Cat # PAB3245 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB3245 - BRD2
Western blot analysis of BRD2 ( arrow ) using rabbit BRD2 polyclonal antibody ( Cat # PAB3245 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the BRD2 gene ( Lane 2 ) ( Origene Technologies ) .
PAB3245 - BRD2
( LEFT ) Western blot analysis of BRD2 polyclonal antibody ( Cat # PAB3245 ) in A-375 cell lysate. BRD2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP14978PU-N - BRD2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP14978PU-N - BRD2
(LEFT)Western blot analysis of anti-BRD2 Pab in A375 cell lysate. BRD2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).
PAB14061 - BRD2
Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Brd2 polyclonal antibody ( Cat # PAB14061 ), diluted 1 : 1,000 in BSA/PBS-Tween.
PAB14061 - BRD2
ELISA was performed using a serial dilution of Brd2 polyclonal antibody ( Cat # PAB14061 ). By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 5,000.
H00006046-D01 - BRD2
Immunoprecipitation of BRD2 transfected lysate using anti-BRD2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BRD2 MaxPab mouse polyclonal antibody (B01) (H00006046-B01).
AR51696PU-N - BRD2
TA335720 - BRD2
WB Suggested Anti-BRD2 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate; BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
TA335721 - BRD2
WB Suggested Anti-BRD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate; BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
TA347084 - BRD2
WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Brd2 , diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (predicted size: 88 kDa) is indicated on the right.
TA347084 - BRD2
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Brd2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BRD2 antibody
  Rabbit Polyclonal Anti-BRD2 Antibody  
WB Suggested Anti-BRD2 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate; BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

BRD2 antibody
  Rabbit Polyclonal Anti-BRD2 Antibody  
WB Suggested Anti-BRD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate; BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €360.00
  OriGene Technologies, Inc.

BRD2 antibody
  Rabbit Polyclonal BRD2 Antibody  
WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Brd2 , diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (predicted size: 88 kDa) is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

BRD2 (Center) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

BRD2 - human gene knockout kit via CRISPR
BRD2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BRD2 - human gene knockout kit via CRISPR
BRD2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

BRD2 - human gene knockout kit via CRISPR
BRD2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

BRD2 - human gene knockout kit via CRISPR
BRD2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BRD2 (1-455, His-tag)
BRD2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

BRD2 (1-455, His-tag)
BRD2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, BRD2 (mGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, BRD2 (mGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,772.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, BRD2 (mGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, BRD2 (mGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,772.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, BRD2 (Myc-DDK tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1 , 200 uL, >10^7 TU/mL
Lenti ORF particles, BRD2 (Myc-DDK tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,597.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, BRD2 (Myc-DDK tagged) - Human bromodomain containing 2 (BRD2), transcript variant 2 , 200 uL, >10^7 TU/mL
Lenti ORF particles, BRD2 (Myc-DDK tagged) - Human bromodomain containing 2 (BRD2), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €1,597.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Brd2 (GFP-tagged) - Mouse bromodomain containing 2 (Brd2), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Brd2 (GFP-tagged) - Mouse bromodomain containing 2 (Brd2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,556.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Brd2 (Myc-DDK-tagged) - Mouse bromodomain containing 2 (Brd2), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Brd2 (Myc-DDK-tagged) - Mouse bromodomain containing 2 (Brd2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,401.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Brd2 (GFP-tagged ORF) - Rat bromodomain containing 2 (Brd2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Brd2 (GFP-tagged ORF) - Rat bromodomain containing 2 (Brd2), 200 uL, >10^7 TU/mL  
200 µl / €1,556.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Brd2 (Myc-DDK-tagged ORF) - Rat bromodomain containing 2 (Brd2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Brd2 (Myc-DDK-tagged ORF) - Rat bromodomain containing 2 (Brd2), 200 uL, >10^7 TU/mL  
200 µl / €1,400.00
  OriGene Technologies, Inc.
 

18 Item(s)

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18 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Human Lenti ORF Particle (4)
  3. Mouse Lenti ORF Particle (2)
  4. Primary Antibody (4)
  5. Protein | Growth Factor (2)
  6. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (4)
Target
  1. BRD2 (6)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (3)
  3. Bovine (Bov) (2)
  4. Canine (Can) (2)
  5. Equine (Eq) (2)
  6. Rat (Rt) (2)
  7. Guinea Pig (GP) (1)
  8. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
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Host
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Images
  1. 1 image (6)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (2)
Source
  1. E. coli (2)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
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  1. OriGene Technologies (18)
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