BRD2 antibody
Principal name
BRD2 antibody
Alternative names for BRD2 antibody
KIAA9001, RING3, O27.1.1, Bromodomain-containing protein 2
Ncbi ID
NP_005095, 9606, 10090, NP_001106653, NP_001106653.1, NP_005095.1, NP_001191902.1, NM_010238, NM_212495, NM_005104, NM_001113182
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of BRD2 antibody
- Kit Component:
- - KN302252G1, Brd2 gRNA vector 1 in pCas-Guide vector
- KN302252G2, Brd2 gRNA vector 2 in pCas-Guide vector
- KN302252D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Boehm,D., et al. (2013) Cell Cycle 12 (3), 452-462Tang,X., et al. (2013) Mol. Pharmacol. 83 (1), 283-293
Human Jurkat; WB Suggested Anti-BRD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; BRD2 antibody - middle region (ARP34212_P050) in Human Jurkat cells using Western Blot
BRD2 monoclonal antibody (M01), clone 3D10. Western Blot analysis of BRD2 expression in Hela NE(Cat # L013V3 ).
Detection limit for recombinant GST tagged BRD2 is approximately 0.03ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-BRD2 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; BRD2 antibody - C-terminal region (ARP34211_T100) in Human Jurkat cells using Western Blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BRD2 polyclonal antibody ( Cat # PAB3245 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of BRD2 ( arrow ) using rabbit BRD2 polyclonal antibody ( Cat # PAB3245 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the BRD2 gene ( Lane 2 ) ( Origene Technologies ) .
( LEFT ) Western blot analysis of BRD2 polyclonal antibody ( Cat # PAB3245 ) in A-375 cell lysate. BRD2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
(LEFT)Western blot analysis of anti-BRD2 Pab in A375 cell lysate. BRD2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).
Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Brd2 polyclonal antibody ( Cat # PAB14061 ), diluted 1 : 1,000 in BSA/PBS-Tween.
ELISA was performed using a serial dilution of Brd2 polyclonal antibody ( Cat # PAB14061 ). By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 5,000.
Immunoprecipitation of BRD2 transfected lysate using anti-BRD2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BRD2 MaxPab mouse polyclonal antibody (B01) (H00006046-B01).
WB Suggested Anti-BRD2 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate; BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
WB Suggested Anti-BRD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate; BRD2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Brd2 , diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (predicted size: 88 kDa) is indicated on the right.
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Brd2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA335720 | BRD2 antibody |
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| Rabbit Polyclonal Anti-BRD2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA335721 | BRD2 antibody |
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| Rabbit Polyclonal Anti-BRD2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Por, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347084 | BRD2 antibody |
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| Rabbit Polyclonal BRD2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Ms | E |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP14978PU-N | BRD2 (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN220612 | BRD2 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN220612BN | BRD2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN220612LP | BRD2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN220612RB | BRD2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| AR51696PU-N | BRD2 (1-455, His-tag) |
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 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| OriGene Technologies GmbH | |||||
| AR51696PU-S | BRD2 (1-455, His-tag) |
||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
| OriGene Technologies GmbH | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC220612L2V | Lenti ORF particles, BRD2 (mGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,772.00
|
|
| OriGene Technologies, Inc. | ||
| RC226173L2V | Lenti ORF particles, BRD2 (mGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,772.00
|
|
| OriGene Technologies, Inc. | ||
| RC220612L1V | Lenti ORF particles, BRD2 (Myc-DDK tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,597.00
|
|
| OriGene Technologies, Inc. | ||
| RC226173L1V | Lenti ORF particles, BRD2 (Myc-DDK tagged) - Human bromodomain containing 2 (BRD2), transcript variant 2 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,597.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR223618L2V | Lenti ORF particles, Brd2 (GFP-tagged) - Mouse bromodomain containing 2 (Brd2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,556.00
|
|
| OriGene Technologies, Inc. | ||
| MR223618L1V | Lenti ORF particles, Brd2 (Myc-DDK-tagged) - Mouse bromodomain containing 2 (Brd2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,401.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211139L2V | Lenti ORF particles, Brd2 (GFP-tagged ORF) - Rat bromodomain containing 2 (Brd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,556.00
|
|
| OriGene Technologies, Inc. | ||
| RR211139L1V | Lenti ORF particles, Brd2 (Myc-DDK-tagged ORF) - Rat bromodomain containing 2 (Brd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,400.00
|
|
| OriGene Technologies, Inc. | ||
