BRF1 / TFIIIB90 antibody

Principal name

BRF1 / TFIIIB90 antibody

Alternative names for BRF1 / TFIIIB90 antibody

BRF, GTF3B, TAF3B2, TAF3C, Transcription factor IIIB 90 kDa subunit, B-related factor 1, TATA box-binding protein-associated factor, RNA polymerase III subunit 2

SwissProt ID

Q8CFK2 (Mouse), Q92994 (Human)

Gene ID

2972 (BRF1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BRF1 / TFIIIB90 antibody

RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in alignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BRF1 / TFIIIB90 antibody

  Rabbit polyclonal anti-TF3B antibody  
Western blot analysis of extracts from HeLa cells and Jurkat cells, using TF3B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BRF1 / TFIIIB90 antibody

  Rabbit Polyclonal Anti-BRF1 Antibody  
WB Suggested Anti-BRF1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BRF1 / TFIIIB90 antibody

  Rabbit Polyclonal Anti-BRF1 Antibody  
WB Suggested Anti-BRF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified GP, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

BRF1 / TFIIIB90 antibody

Immunohistochemistry analysis of TFIIIB90-1 Antibody (Cat.-No AP01180PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BRF1 / TFIIIB90 (Center) antibody

BRF1 Antibody (Center) (Cat. #AP50389PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the BRF1 antibody detected the BRF1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BRF1 / TFIIIB90 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

BRF1 / TFIIIB90 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BRF1 / TFIIIB90 (transcript variant 1)

  Recombinant protein of human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 1  
BRF1 / TFIIIB90 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

BRF1 overexpression lysate

BRF1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, BRF1 (mGFP-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, BRF1 (mGFP-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, BRF1 (Myc-DDK-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, BRF1 (Myc-DDK-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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