Bromodeoxyuridine / BrDU antibody

Principal name

Bromodeoxyuridine / BrDU antibody

Available reactivities

All Species, Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rat, Sheep, Rabbit

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB)

Background of Bromodeoxyuridine / BrDU antibody

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

General readings

1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 
2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 
3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369. 
4. Das, G. et al. (2009) Cyclin D1 fine-tunes the neurogenic output of embryonic retinal progenitor cells. Neural Dev. 
4: 15. 5. Nakhai, H. et al. (2008) Conditional ablation of Notch signaling in pancreatic development. Development. 135: 2757-65. 
6. Ghai, K. et al. (2010) Notch signaling influences neuroprotective and proliferative properties of mature Müller glia. J Neurosci. 30: 3101-12. 
7. Amador-Arjona, A. et al. (2011) Primary cilia regulate proliferation of amplifying progenitors in adult hippocampus: implications for learning and memory. J Neurosci. 31: 9933-44. 
8. Bugler, B. et al. (2010) Unscheduled expression of CDC25B in S-phase leads to replicative stress and DNA damage. Mol Cancer. 9: 29. 
9. Gonzalo-Gobernado, R. et al (2009) Mobilization of neural stem cells and generation of new neurons in 6-OHDA-lesioned rats by intracerebroventricular infusion of liver growth factor. J Histochem Cytochem. 57: 491-502 
10. Xu, Q. et al. (2010) Sonic hedgehog signaling confers ventral telencephalic progenitors with distinct cortical interneuron fates. Neuron. 65: 328-40. 
11. Zhang, J. et al. (2010) A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons. BMC Neurosci. 11: 158. 
12. Bonzo, J.A. et al. (2012) Suppression of Hepatocyte Proliferation by Hepatocyte Nuclear Factor 4a in Adult Mice. J Biol Chem. Jan 12. [Epub ahead of print] 
13. Knopf, F. et al. (2011) Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin. Dev Cell. 20: 713-24. 
14. Grotek, B. et al. (2013) Notch signaling coordinates cellular proliferation with differentiation during zebrafish fin regeneration. Development. 140: 1412-23.
15. Kim, T.H. et al. (2011) Genetic evidence that intestinal notch functions vary regionally and operate through a common mechanism of math1 repression. J Biol Chem. Jan 31. [Epub ahead of print] 
16. Kroehne, V. et al. (2011) Regeneration of the adult zebrafish brain from neurogenic radial glia-type progenitors. Development. 138: 4831-41. 
17. Liu, M.T. et al. (2009) 5-HT4 receptor-mediated neuroprotection and neurogenesis in the enteric nervous system of adult mice. J Neurosci. 29: 9683-99. 
18. Lundgren, O. et al. (2011) Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves. PLoS One. 6: e16295. 
19. Muja, N. et al. (2011) Neural precursors exhibit distinctly different patterns of cell migration upon transplantation during either the acute or chronic phase of EAE: A serial MR imaging study. Magn Reson Med. Feb 8. [Epub ahead of print] 
20. Puverel, S. et al. (2011) RanBPM is essential for mouse spermatogenesis and oogenesis. Development. 138: 2511-21. 
21. Sabo, J.K. et al. (2011) Remyelination is altered by bone morphogenic protein signaling in demyelinated lesions. J Neurosci. 31: 4504-10. 
22. Scheys, J.O. et al. (2011) Evidence of adrenal failure in aging dax1-deficient mice. Endocrinology. 152: 3430-9. 
23. Wang, W. et al. (2013) Extracellular signal-regulated kinase 5 (ERK5) mediates prolactin-stimulated adult neurogenesis in the subventricular zone and olfactory bulb. J Biol Chem. 288: 2623-31 
24. Zemans, R.L. et al. (2013) Role of ß-catenin-Regulated CCN Matricellular Proteins in Epithelial Repair After Inflammatory Lung Injury. Am J Physiol Lung Cell Mol Physiol. Jan 11. [Epub ahead of print] 
25. Zimmerman, K.M. et al. (2013) Diminished origin licensing capacity specifically sensitises tumour cells to replication stress. Mol Cancer Res. Jan 30. [Epub ahead of print] 
26. Maden, M. et al. (2013) Proliferation zones in the axolotl brain and regeneration of the telencephalon. Neural Dev. 8: 1. 
27. Perez-Ruiz A et al. {beta}-catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci : (2008).
28. Iulianella A, Sharma M, Durnin M, Vanden Heuvel GB, Trainor PA. Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis. Development. 2008 Feb;135(4):729-41. doi: 10.1242/dev.013276. PubMed PMID: 18223201.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Bromodeoxyuridine / BrDU antibody

Bromodeoxyuridine / BrDU Mouse IgG1 MoBu-1 FITC C, F
0.1 mg / €420.00
  OriGene Technologies GmbH

Bromodeoxyuridine / BrDU antibody

Bromodeoxyuridine / BrDU Mouse IgG1 BU20a FITC F
50 µg / €250.00
  OriGene Technologies GmbH

Bromodeoxyuridine / BrDU antibody

Bromodeoxyuridine / BrDU Mouse IgG1 BRDU206 FITC F, ICC/IF, P
0.2 mg / €390.00
  OriGene Technologies GmbH

Bromodeoxyuridine / BrDU antibody

Bromodeoxyuridine / BrDU Mouse IgG1 BRDU206 FITC F, ICC/IF, P
0.1 mg / €270.00
  OriGene Technologies GmbH

Bromodeoxyuridine / BrDU antibody

Bromodeoxyuridine / BrDU Mouse IgG2a Bu5.1 FITC ICC/IF
1.5 ml / €310.00
  OriGene Technologies GmbH

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