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BRS3 antibody

Principal name

BRS3 antibody

Alternative names for BRS3 antibody

BRS-3, Bombesin receptor subtype-3

SwissProt ID

O54798 (Mouse), P32247 (Human), Q8K418 (Rat)

Gene ID

680 (BRS3), 12209 (Brs3), 260319

Ncbi ID

9606, NP_001718.1, NP_001718, AAI52939.1, NM_001727, NP_033896.2, NM_009766, NM_152845

Available reactivities

Hu (Human), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Mky (Monkey), Ms (Mouse), Prim (Primate), Rb (Rabbit), Rt (Rat), Sh (Sheep), Bear, Chk (Chicken), Hst (Hamster), Op (Opossum), Xen (Xenopus), Tr (Turkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of BRS3 antibody

Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue. BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP. BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2. The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified. All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth. The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate. Filly, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.

Brain, thalamus: Formalin-Fixed Paraffin-Embedded (FFPE)

AP06858PU-N

Brain, thalamus: Formalin-Fixed Paraffin-Embedded (FFPE)

AP06859PU-N

Uterus, pregnant: Formalin-Fixed Paraffin-Embedded (FFPE)

AP06968PU-N

Western blot (WB) analysis of BRS-3 antibody (Cat.-No.: AP01209PU-N) in extracts from HUVEC cells.

AP01209PU-N

Western blot (WB) analysis of BRS-3 antibody (Cat.-No.: AP01209PU-N) in extracts from A549 and hela cells.

AP01209PU-N

Western blot (WB) analysis of BRS-3 antibody (Cat.-No.: AP01294PU-N) at 1/500 dilution
Lane1:HEK293T cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:H9C2 cell lysate

AP01294PU-N

Immunofluorescence analysis of A549 cells, using BRS3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HuvEc cells, using BRS3 Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of BRS3 Antibody (Center) (Cat. #AP50393PU-N) in mouse stomach tissue lysates (35µg/lane). BRS3 (arrow) was detected using the purified Pab

AP50393PU-N

Western blot analysis of BRS3 expression in MDAMB231 (A); H4 (B); PC3 (C) whole cell lysates.

Western blot analysis of extracts from HUVEC cells, using BRS3 antibody.The lane on the right is treated with the synthesized peptide.

TA313526

Western blot analysis of rat brain membranes: 1. Anti-Bombesin Receptor 3 (extracellular) antibody, (1:200). 2. Anti-Bombesin Receptor 3 (extracellular) antibody, preincubated with the control peptide antigen.

TA328616

Western blot analysis of human malignant melanoma cell line Malme-3M (lanes 1 and 3) and human normal skin fibroblast cell line Malme-3 (lanes 2 and 4): 1, 2. Anti-Bombesin Receptor 3 (extracellular) antibody, (1:500). 3, 4. Anti-Bombesin Receptor 3 (extracellular) antibody, preincubated with the control peptide antigen.

TA328616

Western blot analysis of mouse brain lysate: 1. Anti-Bombesin Receptor 3 (extracellular) antibody, (1:200). 2. Anti-Bombesin Receptor 3 (extracellular) antibody, preincubated with the control peptide antigen.

TA328616

Expression of Bombesin Receptor 3 in rat testes. Immunohistochemical staining of paraffin embedded rat testes section using Anti-Bombesin Receptor 3 (extracellular) antibody, (1:50). The area of the efferent ductules near the epididymis is shown. Intense stain (brown) is specific for the pseudostratified epithelium of the efferent ductules. DAB is used for the color reaction. H&E is used as the counterstain.

TA328616

Indirect flow cytometry analysis in human promyelocytic leukemia HL-60 cells: black line, Unstained cells + goat-anti-rabbit-FITC. green line, Cells + Anti-Bombesin Receptor 3 (extracellular) antibody, (5 µg) + goat-anti-rabbit-FITC.

TA328616

Anti-BRS3 antibody IHC of human placenta, villi. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340505

Anti-BRS3 antibody IHC of human pregnant uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340505

Anti-BRS3 antibody IHC of human placenta, villi. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340506

Anti-BRS3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340865

Anti-BRS3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340878

Anti-BRS3 antibody IHC of human uterus, pregnant. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340945

Brain: Formalin-Fixed Paraffin-Embedded (FFPE)

TA341167

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A172 cells, Primary antibody: (BRS3 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds

TA351014

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (BRS3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351014

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (BRS3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

TA351014

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA328616	BRS3 antibody
	Rabbit Polyclonal Anti-Bombesin Receptor 3 (extracellular)
	Rabbit		Purified	Hu, Ms, Rt	WB	0.2 ml / €435.00
						OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
TA313526	BRS3 antibody
	Rabbit polyclonal anti-BRS3 antibody
	Rabbit	IgG	Purified	Hu	E, WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
TA340505	BRS3 (N-term) antibody
	Rabbit Polyclonal Anti-BRS3 Antibody (N-Terminus)
	Rabbit		Aff - Purified	Hu, Ms, Rt	P	50 µg / €400.00
						OriGene Technologies, Inc.
+1 additional image
TA340506	BRS3 (C-term) antibody
	Rabbit Polyclonal Anti-BRS3 Antibody (C-Terminus)
	Rabbit		Aff - Purified	Hu	P	50 µg / €400.00
						OriGene Technologies, Inc.
TA340865	BRS3 (2nd Extracell. Dom.) antibody
	Rabbit Polyclonal Anti-BRS3 Antibody (Extracellular Domain)
	Rabbit		Aff - Purified	Hu, Mky, Prim	P	50 µg / €400.00
						OriGene Technologies, Inc.
TA340878	BRS3 (C-term, cytopl. dom.) antibody
	Rabbit Polyclonal Anti-BRS3 Antibody (C-Terminus)
	Rabbit		Aff - Purified	Bt, Elephant, Hu, Mky, Prim	P	50 µg / €400.00
						OriGene Technologies, Inc.
TA340945	BRS3 antibody
	Rabbit Polyclonal Anti-BRS3 Antibody (Extracellular Domain)
	Rabbit		Purified	Hu		50 µg / €400.00
						OriGene Technologies, Inc.
TA341167	BRS3 (3rd cytopl. dom.) antibody
	Rabbit Polyclonal Anti-BRS3 Antibody (Cytoplasmic Domain)
	Rabbit		Aff - Purified	Bear, Bov, Bt, Can, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Prim, Rb, Rt, Sh	P	50 µg / €400.00
						OriGene Technologies, Inc.
TA351014	BRS3 antibody
	Rabbit Polyclonal Anti-BRS3 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
+2 additional images
AP01209PU-N	BRS3 antibody
	Rabbit		Aff - Purified	Hu	ICC/IF, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
+1 additional image
AP01294PU-N	BRS3 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
AP06858PU-N	BRS3 (N-term extracell. dom.) antibody
	Rabbit		Aff - Purified	Bear, Bov, Bt, Can, Eq, GP, Hu, Mky, Ms, Prim, Rb, Rt, Sh	E, P	50 µg / €470.00
						Acris Antibodies GmbH
AP06859PU-N	BRS3 (C-term cytopl. dom.) antibody
	Rabbit		Aff - Purified	Hu, Mky, Prim	E, P	50 µg / €470.00
						Acris Antibodies GmbH
AP06968PU-N	BRS3 (1st Cytopl. Dom.) antibody
	Rabbit		Aff - Purified	Bear, Bov, Bt, Can, Chk, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Sh, Tr, Xen	E, P	50 µg / €470.00
						Acris Antibodies GmbH
AP50393PU-N	BRS3 (Center) antibody
	Rabbit	Ig	Aff - Purified	Hu, Ms	WB	0.4 ml / €370.00
						Acris Antibodies GmbH
G034-1	BRS3 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	50 µg / €230.00
						Assay Biotech
+1 additional image
G034-2	BRS3 antibody
	Rabbit		Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €330.00
						Assay Biotech
+1 additional image
G217-1	BRS3 antibody
	Rabbit		Aff - Purified	Hu	E, WB	50 µg / €230.00
						Assay Biotech
G217-2	BRS3 antibody
	Rabbit		Aff - Purified	Hu	E, WB	0.1 mg / €330.00
						Assay Biotech