BTN3A2 antibody
Principal name
BTN3A2 antibody
Alternative names for BTN3A2 antibody
Butyrophilin subfamily 3 member A2, BT3.2, BTF3, BTF4
SwissProt ID
P78410 (Human)
Gene ID
11118 (BTN3A2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of BTN3A2 antibody
This gene encodes a member of the immunoglobulin superfamily, containing two Ig domains with similarity to Ig variable and Ig constant domains. The gene resides in the juxta-telomeric region of the major histocompatability class 1 locus on chromosome 6 in the seven member BTN cluster, which includes butyrophilin, and three members each of the BTN2 and BTN3 subfamilies. Alternatively spliced transcript variants have been described but their full-length nature has yet to be determined.
Immunohistochemistry: BTN3A2 Antibody [28010002] - Most of the normal tissues displayed moderate cytoplasmic positivity with an additional membranous staining in gastro-intestinal tract. Smooth muscle cells and lymphoid cells outside reaction centra showed strong cytoplasmic staining while strong nuclear staining was seen in a fraction of cells in seminiferous duct. Liver, renal tubules, prostate glands and neuronal tissues were weakly stained or negative. Malignant tissues exhibited similar staining properties as normal cells, mainly moderate cytoplasmic and/or membranous immunoreactivity. Many gliomas, prostate, skin, testicular, urothelial and renal cancers were weakly stained or negative. Image and statement courtesy of the Human Protein Atlas (HPA).
Western Blot: BTN3A2 Antibody [28010002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 36 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
ELISA: BTN3A2 Antibody [28010002]
BTN3A2 MaxPab polyclonal antibody. Western Blot analysis of BTN3A2 expression in human spleen.
Western Blot analysis of BTN3A2 expression in transfected 293T cell line (H00011118-T01) by BTN3A2 MaxPab polyclonal antibody.Lane 1: BTN3A2 transfected lysate(36.74 KDa).Lane 2: Non-transfected lysate.
Western Blot: BTN3A2 Lysate [NBL1-08054] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BTN3A2
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BTN3A2 mouse monoclonal antibody. (GTX84773, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BTN3A2 mouse monoclonal antibody. (GTX84773, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BTN3A2 mouse monoclonal antibody. (GTX84773, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue using anti-BTN3A2 mouse monoclonal antibody. (GTX84773, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue using anti-BTN3A2 mouse monoclonal antibody. (GTX84773, Dilution 1:50)
Immunofluorescent staining of HT29 cells using anti-BTN3A2 mouse monoclonal antibody (GTX84773).
Anti-BTN3A2 mouse monoclonal antibody (GTX84773) immunofluorescent staining of COS7 cells transiently transfected with BTN3A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2 .
Immunohistochemical staining of paraffin-embedded breast tissue using anti-BTN3A2 mouse monoclonal antibody. (GTX84773, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500730, Dilution 1:50)
Immunofluorescent staining of HT29 cells using anti-BTN3A2 mouse monoclonal antibody (TA500730).
Anti-BTN3A2 mouse monoclonal antibody (TA500730) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN3A2 (RC201183).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2 .
Figure from citation: Vg2Vd2 T cells proliferate in response to the 20.1 but not the 1A6 mAb, although both are specific for BTN3. The 12G12 Vg2Vd2 T cell clone was cultured with control IgG1 mAb, BTN3A2(1A6) mAb, or 20.1 mAb in the presence of mitomycin C–treated Va2 cells. The cells were pulsed with [3H]thymidine after 24 h and harvested 18 h later. View Citation
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTN3A2 rabbit polyclonal antibody. (TA590233)
Immunohistochemistry: BTN3A2 Antibody - Most of the normal tissues displayed moderate cytoplasmic positivity with an additional membranous staining in gastro-intestinal tract. Smooth muscle cells and lymphoid cells outside reaction centra showed strong cytoplasmic staining while strong nuclear staining was seen in a fraction of cells in seminiferous duct. Liver, renal tubules, prostate glands and neuronal tissues were weakly stained or negative. Malignant tissues exhibited similar staining properties as normal cells, mainly moderate cytoplasmic and/or membranous immunoreactivity. Many gliomas, prostate, skin, testicular, urothelial and renal cancers were weakly stained or negative. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BTN3A2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: BTN3A2 Antibody
- Quick-Filters:
- Cited (2)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500730 | BTN3A2 antibody |
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Mouse | IgG1 | OTI1A6 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Published recently! Highly validated antibody!
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| TA590233 | BTN3A2 antibody |
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Rabbit | IgG | Purified | Can, Hu, Ms | E, ICC/IF, P, WB |
50 µg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody! |
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| H00011118-B01P | BTN3A2 antibody |
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Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| 28010002 | BTN3A2 antibody |
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![Immunohistochemistry: BTN3A2 Antibody [28010002] - Most of the normal tissues displayed moderate cytoplasmic positivity with an additional membranous staining in gastro-intestinal tract. Smooth muscle cells and lymphoid cells outside reaction centra showed strong cytoplasmic staining while strong nuclear staining was seen in a fraction of cells in seminiferous duct. Liver, renal tubules, prostate glands and neuronal tissues were weakly stained or negative. Malignant tissues exhibited similar staining properties as normal cells, mainly moderate cytoplasmic and/or membranous immunoreactivity. Many gliomas, prostate, skin, testicular, urothelial and renal cancers were weakly stained or negative. Image and statement courtesy of the Human Protein Atlas (HPA).](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/28010002=IHC_Courtesy_of_HPA.jpg) |
Rabbit | Hu | E, P, WB |
50 µg /
€400.00
|
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| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301183 | BTN3A2 |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
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|
| OriGene Technologies, Inc. | |||||
Published recently! |
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| TP721116 | BTN3A2 (transcript variant 1) |
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€139.00
|
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| OriGene Technologies, Inc. | |||||
| H00011118-P01-10 | BTN3A2 |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
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|
| Abnova Taiwan Corp. | |||||
| H00011118-P01-25 | BTN3A2 |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
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| Abnova Taiwan Corp. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| H00011118-T01 | BTN3A2 293T Cell Transient Overexpression Lysate(Denatured) |
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0.1 ml /
€270.00
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| Abnova Taiwan Corp. |
